14-3-3 σ Antibody (E-11): sc-166473

14-3-3 σ Antibody (E-11) is a mouse monoclonal IgG3 kappa light chain antibody that detects 14-3-3 sigma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-14-3-3 sigma antibody (E-11) is available as the non-conjugated format. 14-3-3 sigma protein, also known as SFN, stratifin, HME1, or YWHAS, plays a crucial role in regulating various cellular processes, particularly in cancer biology, where 14-3-3 sigma protein is involved in apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 sigma protein is predominantly expressed in stratified squamous keratinizing epithelium and functions as a secreted adaptor protein that interacts with a wide range of target proteins, including Keratin 17 (KRT17). 14-3-3 sigma protein binding to KRT17 enhances the Akt/mTOR signaling pathway, promoting protein synthesis and cell growth. 14-3-3 sigma protein positively mediates insulin-like growth factor I-induced cell cycle progression and interacts with translation initiation factors, thereby regulating mitotic translation. In tumor growth, 14-3-3 sigma protein positively regulates tumor suppressor p53, increasing p53-mediated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 sigma protein, resulting from alternative splicing events, further underscore the complexity and importance in cellular signaling and cancer development.

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14-3-3 σ Antibody (E-11) References:

1. Epigenetic and proteolytic inactivation of 14-3-3sigma in breast and prostate cancers.  |  Horie-Inoue, K. and Inoue, S. 2006. Semin Cancer Biol. 16: 235-9. PMID: 16682214
2. Regulation of the p53-MDM2 pathway by 14-3-3 sigma and other proteins.  |  Lee, MH. and Lozano, G. 2006. Semin Cancer Biol. 16: 225-34. PMID: 16697215
3. Epigenetic silencing of 14-3-3sigma in cancer.  |  Lodygin, D. and Hermeking, H. 2006. Semin Cancer Biol. 16: 214-24. PMID: 16698281
4. The role and regulatory mechanism of 14-3-3 sigma in human breast cancer.  |  Ko, S., et al. 2014. J Breast Cancer. 17: 207-18. PMID: 25320618
5. 14-3-3σ Contributes to Radioresistance By Regulating DNA Repair and Cell Cycle via PARP1 and CHK2.  |  Chen, Y., et al. 2017. Mol Cancer Res. 15: 418-428. PMID: 28087741
6. 14-3-3σ Functions as an Intestinal Tumor Suppressor.  |  Winter, M., et al. 2021. Cancer Res. 81: 3621-3634. PMID: 34224368
7. 14-3-3 Sigma Protein Contributes to Hepatocyte Growth Factor-mediated Cell Proliferation and Invasion via Matrix Metalloproteinase-1 Regulation in Human Gastric Cancer.  |  Jung, JY., et al. 2022. Anticancer Res. 42: 519-530. PMID: 34969762
8. ZLM-7 Blocks Breast Cancer Progression by Inhibiting MDM2 via Upregulation of 14-3-3 Sigma.  |  Wen, M., et al. 2022. Pharmaceuticals (Basel). 15: PMID: 35890172
9. Isoform-Selective Fluorescent Labeling of 14-3-3σ by Acrylamide-Containing Fusicoccins.  |  Tanaka, K., et al. 2023. Chemistry. 29: e202301059. PMID: 37170712
10. Development of a NanoBRET assay for evaluation of 14-3-3σ molecular glues.  |  Vickery, HR., et al. 2024. SLAS Discov. 29: 100165. PMID: 38797286

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-14-3-3 σ Antibody (E-11) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-14-3-3 σ Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

14-3-3 σ Antibody (E-11) Specifications

Alternate Product Name: 14-3-3 sigma

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 25-38

Epitope Target: 14-3-3 σ

Epitope Species: human

Product Reactivity/Detection: 14-3-3 σ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for 14-3-3 σ Antibody (E-11): P: Blocking Peptide

The preferred 14-3-3 σ antibody is 14-3-3 σ Antibody (5D7): sc-100638.