14-3-3 sigma Antibody (E-11) recommended for detection of 14-3-3 σ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

14-3-3 σ Antibody (E-11): sc-166473

14-3-3 σ Antibody (E-11) is rated 3.6 out of 5 by 7.
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Datasheets

    • Anti-14-3-3 σ Antibody (E-11) is a mouse monoclonal IgG3 (kappa light chain) 14-3-3 σ antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 25-38 at the N-terminus of 14-3-3 σ of human origin
    • recommended for detection of 14-3-3 σ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • blocking peptide, sc-166473 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See pan 14-3-3 (B-8): sc-133233 for 14-3-3 σ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 14-3-3 σ (E-11): sc-166473.
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What application is the blocking peptide sc-166473 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from No band We used this antibody detect nasopharyngeal carcinoma cells ,but got no band although the dilution set at 1:100.However, santa cruz is very good service, they give us replacement.
Date published: 2018-01-27
Rated 2 out of 5 by from NO detection in mouse cells I tested free sample on thymic epithelial origin cell lines and got no bands around 30kda, there were strong non-specific bands around 75kda instead.
Date published: 2017-11-14
Rated 1 out of 5 by from Not well work in WB too many unspecific WB bands are detected in human liver cancer cell line BEL-7404 lysate. Dilution ratio is1:100.
Date published: 2017-10-11
Rated 5 out of 5 by from Nice dark western band We used this antibody in a western blot and got a dark band at the correct molecular weight
Date published: 2017-01-24
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in 14-3-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Works well for WB with A Works well for WB with A-431 whole cell lysate. -SCBT QC
Date published: 2015-01-29
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