14-3-3 σ Antibody (5D7): sc-100638

14-3-3 σ Antibody (5D7) is a mouse monoclonal IgG1 kappa light chain antibody that detects 14-3-3 σ of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-14-3-3 σ antibody (5D7) is available in a non-conjugated form, providing flexibility for various experimental needs. 14-3-3 σ, also known as SFN, stratifin, HME1, or YWHAS, plays a crucial role in cellular signaling and regulation, particularly in cancer biology. 14-3-3 σ is predominantly expressed in stratified squamous keratinizing epithelium and functions as a secreted adaptor protein that interacts with various target proteins, including Keratin 17. 14-3-3 σ binding to Keratin 17 enhances the Akt/mTOR signaling pathway, promoting protein synthesis and cell growth, which are vital processes in tumor development and progression. 14-3-3 σ positively regulates tumor suppressor p53, influencing cell cycle checkpoints and apoptosis. This modulation of key pathways highlights 14-3-3 σ′s importance in maintaining cellular homeostasis and potential as a therapeutic target in cancer treatment. 14-3-3 σ monoclonal antibody (5D7) serves as an essential tool for researchers studying cancer biology and related fields.

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14-3-3 σ Antibody (5D7) References:

1. 14-3-3 sigma positively regulates p53 and suppresses tumor growth.  |  Yang, HY., et al. 2003. Mol Cell Biol. 23: 7096-107. PMID: 14517281
2. A structural basis for 14-3-3sigma functional specificity.  |  Wilker, EW., et al. 2005. J Biol Chem. 280: 18891-8. PMID: 15731107
3. 14-3-3sigma, a p53 regulator, suppresses tumor growth of nasopharyngeal carcinoma.  |  Yang, H., et al. 2006. Mol Cancer Ther. 5: 253-60. PMID: 16505098
4. CpG island promoter methylation and silencing of 14-3-3sigma gene expression in LNCaP and Tramp-C1 prostate cancer cell lines is associated with methyl-CpG-binding protein MBD2.  |  Pulukuri, SM. and Rao, JS. 2006. Oncogene. 25: 4559-72. PMID: 16786000
5. 14-3-3sigma controls mitotic translation to facilitate cytokinesis.  |  Wilker, EW., et al. 2007. Nature. 446: 329-32. PMID: 17361185
6. 14-3-3 sigma isoform interacts with the cytoplasmic domain of the transmembrane BP180 in keratinocytes.  |  Li, Y., et al. 2007. J Cell Physiol. 212: 675-81. PMID: 17443672
7. Methylation and intratumoural heterogeneity of 14-3-3 sigma in oral cancer.  |  Bhawal, UK., et al. 2007. Oncol Rep. 18: 817-24. PMID: 17786341
8. Over-expression of 14-3-3sigma in budding colorectal cancer cells modulates cell migration in the presence of tenascin-C.  |  Ide, M., et al. 2007. Oncol Rep. 18: 1451-6. PMID: 17982629
9. DNA methylation patterns of the CDH1, RARB, and SFN genes in choroid plexus tumors.  |  Losi-Guembarovski, R., et al. 2007. Cancer Genet Cytogenet. 179: 140-5. PMID: 18036402
10. Detection of 14-3-3 sigma (σ) promoter methylation as a noninvasive biomarker using blood samples for breast cancer diagnosis.  |  Ye, M., et al. 2017. Oncotarget. 8: 9230-9242. PMID: 27999208
11. 14-3-3σ and Its Modulators in Cancer.  |  Aljabal, G. and Yap, BK. 2020. Pharmaceuticals (Basel). 13: PMID: 33287252
12. 14-3-3σ-NEDD4L axis promotes ubiquitination and degradation of HIF-1α in colorectal cancer.  |  Liu, S., et al. 2023. Cell Rep. 42: 112870. PMID: 37494179

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-14-3-3 σ Antibody (5D7) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-14-3-3 σ Antibody (5D7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

14-3-3 σ Antibody (5D7) Specifications

Alternate Product Name: 14-3-3 sigma

Product Clone Name: 5D7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-249

Epitope Target: 14-3-3 σ

Epitope Species: human

Product Reactivity/Detection: 14-3-3 σ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for 14-3-3 σ Antibody (5D7): N/A

The preferred 14-3-3 σ antibody is 14-3-3 σ Antibody (E-11): sc-166473.