Topo IIβ Antibody (A-12): sc-365071

Topo IIβ Antibody (A-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Topo IIβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Topo IIβ antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Topo IIβ (A-12) monoclonal antibody recognizes a protein that plays a crucial role in maintaining DNA integrity during critical cellular processes such as replication and transcription by introducing transient double-strand breaks in DNA, allowing for relaxation of supercoiled DNA structures. This function prevents DNA tangling and ensures proper chromosome segregation during cell division, which is essential for genomic stability. Differential expression of Topo IIβ, particularly in specific tissues, suggests unique regulatory roles in cellular processes compared to Topo IIα isoform. Understanding distinct functions of Topo IIβ can provide insights into potential therapeutic targets in cancer treatment, where modulation of topoisomerase activity is a common strategy for disrupting cancer cell proliferation.

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Topo IIβ Antibody (A-12) References:

1. DNA topoisomerase IIbeta and neural development.  |  Yang, X., et al. 2000. Science. 287: 131-4. PMID: 10615047
2. Involvement of DNA topoisomerase IIbeta in neuronal differentiation.  |  Tsutsui, K., et al. 2001. J Biol Chem. 276: 5769-78. PMID: 11106659
3. Topoisomerase IIbeta activates a subset of neuronal genes that are repressed in AT-rich genomic environment.  |  Sano, K., et al. 2008. PLoS One. 3: e4103. PMID: 19116664
4. Novel ametantrone-amsacrine related hybrids as topoisomerase IIβ poisons and cytotoxic agents.  |  Zagotto, G., et al. 2014. Arch Pharm (Weinheim). 347: 728-37. PMID: 25042690
5. DNA topoisomerase IIβ stimulates neurite outgrowth in neural differentiated human mesenchymal stem cells through regulation of Rho-GTPases (RhoA/Rock2 pathway) and Nurr1 expression.  |  Zaim, M. and Isik, S. 2018. Stem Cell Res Ther. 9: 114. PMID: 29695291
6. Mutations in topoisomerase IIβ result in a B cell immunodeficiency.  |  Broderick, L., et al. 2019. Nat Commun. 10: 3644. PMID: 31409799
7. The Role of Topoisomerase IIβ in the Mechanisms of Action of the Doxorubicin Cardioprotective Agent Dexrazoxane.  |  Hasinoff, BB., et al. 2020. Cardiovasc Toxicol. 20: 312-320. PMID: 31773441
8. Down regulation of DNA topoisomerase IIβ exerts neurodegeneration like effect through Rho GTPases in cellular model of Parkinson's disease by Down regulating tyrosine hydroxylase.  |  Yeman, KB. and Isik, S. 2021. Neurol Res. 43: 464-473. PMID: 33402057
9. Genome-wide prediction of topoisomerase IIβ binding by architectural factors and chromatin accessibility.  |  Martínez-García, PM., et al. 2021. PLoS Comput Biol. 17: e1007814. PMID: 33465072
10. Structure-Activity Relationship Study of Dexrazoxane Analogues Reveals ICRF-193 as the Most Potent Bisdioxopiperazine against Anthracycline Toxicity to Cardiomyocytes Due to Its Strong Topoisomerase IIβ Interactions.  |  Jirkovská, A., et al. 2021. J Med Chem. 64: 3997-4019. PMID: 33750129

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Topo IIβ Antibody (A-12) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Topo IIβ Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Topo IIβ Antibody (A-12) Specifications

Alternate Product Name: DNA topoisomerase II beta

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1579-1611

Epitope Target: Topo IIβ

Epitope Species: human

Product Reactivity/Detection: Topo IIβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Topo IIβ Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Topo IIβ antibody is Topo IIβ Antibody (H-8): sc-25330.