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caspase-9 Antibody (3C122) - Western Blotting - Image 17527
caspase-9 Antibody (3C122): sc-70505. Western blot analysis of procaspase-9 expression in CCRF-CEM (A), HeLa (B) and MOLT-4 (C) whole cell lysates.
caspase-9 Antibody (3C122) - Western Blotting - Image 17527
caspase-9 Antibody (3C122): sc-70505. Western blot analysis of procaspase-9 expression in CCRF-CEM (A), HeLa (B) and MOLT-4 (C) whole cell lysates.
caspase-9 Antibody (3C122) - Western Blotting - Image 379981
caspase-9 Antibody (3C122): sc-70505. Western blot analysis of procaspase-9 expression in Jurkat (A), K-562 (B) and U-87 MG (C) whole cell lysates.
caspase-9 Antibody (3C122) - Western Blotting - Image 17124
caspase-9 Antibody (3C122): sc-70505. Western blot analysis of procaspase-9 expression in HeLa (A), Jurkat (B), HuT 78 (C), MOLT-4 (D), UV-treated HeLa (E) and staurosporine-treated HeLa (F) whole cell lysates.
caspase-9 Antibody (3C122): sc-70505. Western blot analysis of procaspase-9 expression in CCRF-CEM (A), HeLa (B) and MOLT-4 (C) whole cell lysates.

caspase-9 Antibody (3C122): sc-70505

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Datasheets
  • caspase-9 Antibody (3C122) is a mouse monoclonal IgG1 κ, cited in 8 publications, provided at 200 µg/ml
  • raised against the prodomain of caspase-9 of human origin
  • recommended for detection of caspase-9 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See caspase-9 (96.1.23): sc-56076 for caspase-9 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for caspase-9 Antibody (3C122) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-9 Antibody (3C122) (see ordering information below).
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Additional Caspase Antibodies including caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5, caspase-6, caspase-7 and caspase-8

caspase-9 Antibody (3C122) is a mouse monoclonal IgG1 antibody that detects caspase-9 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Caspase-9 plays a pivotal role in the intrinsic pathway of apoptosis, acting as an initiator caspase that is activated in response to cellular stress and mitochondrial dysfunction. Upon activation, caspase-9 cleaves and activates downstream effector caspases, such as caspase-3 and caspase-7, leading to the execution phase of apoptosis. This process is crucial for maintaining cellular homeostasis and eliminating damaged or unwanted cells, thereby preventing the development of various diseases, including cancer. The activation of caspase-9 is tightly regulated and can be influenced by various post-translational modifications, such as phosphorylation and ubiquitination, which can affect its activity and stability. Understanding the function and regulation of caspase-9 is essential for elucidating the mechanisms of apoptosis and developing therapeutic strategies for diseases characterized by dysregulated cell death. Anti-caspase-9 antibody (3C122) serves as an invaluable tool for researchers studying apoptosis and its implications in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

caspase-9 Antibody (3C122) References:

  1. Caspase-9 activation and caspase cleavage of tau in the Alzheimer's disease brain.  |  Rohn, TT., et al. 2002. Neurobiol Dis. 11: 341-54. PMID: 12505426
  2. Effect of 103Pd radioactive stent on caspase-9, cholangiocarcinoma cell growth and its radiosensitivity.  |  Guijin, H., et al. 2011. Surg Oncol. 20: 247-51. PMID: 20231087
  3. Caspase-9 mediates the apoptotic death of megakaryocytes and platelets, but is dispensable for their generation and function.  |  White, MJ., et al. 2012. Blood. 119: 4283-90. PMID: 22294729
  4. Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9.  |  Hu, Q., et al. 2013. J Biol Chem. 288: 15142-7. PMID: 23572523
  5. An epistatic effect of apaf-1 and caspase-9 on chlamydial infection.  |  Rahman, MA., et al. 2015. Apoptosis. 20: 1271-80. PMID: 26290316
  6. Simvastatin pretreatment reduces caspase-9 and RIPK1 protein activity in rat cardiac allograft ischemia-reperfusion.  |  Tuuminen, R., et al. 2016. Transpl Immunol. 37: 40-45. PMID: 27155462
  7. Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth.  |  Qian, H., et al. 2018. Mol Med Rep. 18: 1067-1073. PMID: 29845240
  8. STAT3 activates the anti-apoptotic form of caspase 9 in oncovirus-infected B lymphocytes.  |  Koganti, S., et al. 2020. Virology. 540: 160-164. PMID: 31928997
  9. Proximity-induced caspase-9 activation on a DNA origami-based synthetic apoptosome.  |  Rosier, BJHM., et al. 2020. Nat Catal. 3: 295-306. PMID: 32190819
  10. Major Contribution of Caspase-9 to Honokiol-Induced Apoptotic Insults to Human Drug-Resistant Glioblastoma Cells.  |  Wu, GJ., et al. 2020. Molecules. 25: PMID: 32210117

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

caspase-9 Antibody (3C122)

sc-70505
200 µg/ml
$322.00

caspase-9 Antibody (3C122): m-IgG Fc BP-HRP Bundle

sc-539286
200 µg Ab; 10 µg BP
$361.00

Can sc-70505: caspase-9 (3C122) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-70505: caspase-9 (3C122) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by ET from Works well in Western Blot in HeLaWorks well in Western Blot in HeLa, MCF-7, PC3 cells -SCBT Publication Review
Date published: 2009-01-29
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caspase-9 Antibody (3C122) is rated 5.0 out of 5 by 1.
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