VEZF1 Activators
Chemical activators of VEZF1 employ a variety of mechanisms to enhance the protein's ability to bind to DNA and activate transcription. Zinc, serving as a cofactor, directly facilitates VEZF1's DNA-binding capacity. The presence of magnesium is critical for maintaining the structural stability of VEZF1, ensuring that it retains the proper shape required for binding to DNA. Manganese ions, by acting as cofactors, can also enhance VEZF1's conformation and, consequently, its interaction with DNA. Similarly, copper can stabilize the tertiary structures of VEZF1, thereby aiding in its DNA binding and activation. Iron plays a slightly different role; it often acts as a cofactor for enzymes that modify transcription factors and thus may influence VEZF1's function through changes in its phosphorylation state.
Furthermore, cobalt can stabilize VEZF1's structure, enhancing its ability to bind to DNA. Although nickel is not commonly seen as a cofactor, it can influence VEZF1's folding and function, potentially leading to an enhanced activation state. Calcium's role is more indirect; it participates in cellular signaling that can lead to the activation of VEZF1 through secondary messengers and kinases, which aid in VEZF1's ability to initiate transcription. Sodium orthovanadate helps maintain VEZF1 in an activated state by inhibiting phosphatases that would otherwise dephosphorylate and inactivate it. Forskolin increases intracellular cAMP levels, activating PKA, which can then phosphorylate VEZF1, promoting its activation. Phorbol 12-myristate 13-acetate (PMA) activates PKC, which is known to phosphorylate substrates including transcription factors, hence possibly enhancing VEZF1's activity. Lastly, 5-Azacytidine indirectly affects VEZF1 by reducing DNA methylation, which can lead to a more transcriptionally active chromatin state, thereby facilitating VEZF1's access to its target DNA sequences.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc can serve as a cofactor for VEZF1, facilitating its DNA-binding capacity and thus enhancing its transcriptional activation function. | ||||||
Manganese | 7439-96-5 | sc-250292 | 100 g | $270.00 | ||
Manganese ions can act as cofactors for various DNA-binding proteins. By binding to VEZF1, they can enhance its conformation for effective interaction with DNA, thereby activating VEZF1's function. | ||||||
Copper | 7440-50-8 | sc-211129 | 100 g | $51.00 | ||
Copper can interact with certain transcription factors and potentially stabilize the tertiary structures of proteins like VEZF1, enhancing their DNA binding and activation capacity. | ||||||
Iron | 7439-89-6 | sc-215190 sc-215190A | 500 g 2 kg | $69.00 $180.00 | ||
Iron may participate as a cofactor for certain enzymes that can post-translationally modify transcription factors. By doing so, it can facilitate the functional activation of VEZF1 through changes in its phosphorylation state, impacting its DNA binding and regulatory functions. | ||||||
Calcium | 7440-70-2 | sc-252536 | 5 g | $209.00 | ||
Calcium ions play a pivotal role in cellular signaling and can influence the activity of various proteins. By altering the signaling environment, calcium can promote the activation of VEZF1 through secondary messengers and kinases that increase VEZF1's ability to bind to DNA and activate transcription. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $49.00 $57.00 $187.00 | 142 | |
As a phosphatase inhibitor, sodium orthovanadate can prevent dephosphorylation of transcription factors. Phosphorylation often regulates the activity of proteins like VEZF1, and by inhibiting phosphatases, sodium orthovanadate can sustain the phosphorylated, and thus activated, state of VEZF1. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which is involved in the phosphorylation of many substrates, including transcription factors. PKC-mediated phosphorylation can enhance the DNA-binding capability of VEZF1, leading to its activation. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
5-Azacytidine, a DNA methyltransferase inhibitor, can indirectly lead to the activation of certain genes by reducing methylation. While not directly activating VEZF1, reduced methylation can lead to a more open chromatin state, increasing VEZF1’s accessibility to its target DNA sequences and thus enhancing its activation. | ||||||