RuvA Activators
RuvA, a central player in bacterial DNA recombination and repair, is intricately associated with the processes ensuring genomic integrity. Given its role in promoting Holliday junction migration during homologous recombination, understanding chemicals that can activate RuvA becomes critical. Most of the chemicals, like Mitomycin C, Ciprofloxacin, Norfloxacin, and Bleomycin, function by inducing DNA damage, a common distress signal for bacteria. This damage invariably leads to the activation of the SOS response, a well-characterized bacterial stress response mechanism. This response marshals a suite of proteins, including RuvA, to address the compromised DNA integrity.
Another category includes agents like Nalidixic acid and Hydroxyurea, which directly or indirectly stall the DNA replication machinery. Stalled replication forks present a unique challenge, often requiring proteins like RuvA to resolve the ensuing DNA structures. Aflatoxin B1, 4-Nitroquinoline N-oxide, MMS, 2-Aminopurine, and Acridine Orange, albeit varied in their modes of action, result in DNA aberrations that can elicit the SOS response. The cellular consequence of this response, among other actions, can be an upregulation of RuvA, highlighting its central role in bacterial DNA repair and maintenance. Understanding these activators and their mechanisms offers a clearer insight into the cellular responses and the pivotal role RuvA plays therein.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Ciprofloxacin | 85721-33-1 | sc-217900 | 1 g | $43.00 | 8 | |
Ciprofloxacin interferes with bacterial DNA gyrase. This interference leads to DNA damage, subsequently inducing the SOS response. The induction can lead to a potential increase in RuvA levels as the bacterium attempts to repair damaged DNA. | ||||||
Norfloxacin | 70458-96-7 | sc-215586 | 10 g | $125.00 | 1 | |
Norfloxacin, like Ciprofloxacin, affects bacterial DNA gyrase, inducing DNA damage and triggering the SOS response. The activated SOS response can raise RuvA levels to facilitate DNA repair processes. | ||||||
Bleomycin | 11056-06-7 | sc-507293 | 5 mg | $275.00 | 5 | |
Bleomycin induces DNA breaks, leading to the activation of the bacterial SOS response. The heightened SOS response can potentially increase RuvA levels to address DNA damage. | ||||||
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $78.00 $260.00 | 18 | |
Hydroxyurea causes DNA replication fork stalling. The stalled forks can activate the SOS response in bacteria, potentially elevating RuvA levels to aid in resolving stalled replication forks. | ||||||
4-Nitroquinoline N-oxide | 56-57-5 | sc-256815 sc-256815A | 1 g 5 g | $124.00 $421.00 | 6 | |
This chemical is a mutagen causing DNA damage, leading to the induction of the SOS response. This activation can raise the levels of RuvA as part of the DNA repair process. | ||||||
Methyl methanesulfonate | 66-27-3 | sc-250376 sc-250376A | 5 g 25 g | $56.00 $133.00 | 2 | |
MMS is an alkylating agent causing DNA damage. The resulting damage activates the SOS response, potentially increasing RuvA levels to facilitate DNA repair mechanisms. | ||||||
2-Aminopurine | 452-06-2 | sc-287828 sc-287828A | 100 mg 250 mg | $118.00 $185.00 | ||
2-Aminopurine is a base analog inducing DNA mutations. This can activate the bacterial SOS response, leading to a potential increase in RuvA levels to deal with the aberrant DNA structures. | ||||||
Acridine Orange solution | 65-61-2 | sc-473594 | 10 ml | $166.00 | 2 | |
Acridine Orange intercalates into DNA, causing frameshift mutations. The induced DNA damage can activate the SOS response, potentially increasing RuvA levels for DNA repair. | ||||||