RuvA Antibody (12C6): sc-53435
- RuvA Antibody (12C6) is a mouse monoclonal IgG1 κ RuvA antibody provided at 200 µg/ml
- raised against Ruv A protein from E. coli
- RuvA Antibody (12C6) is recommended for detection of RuvA of E. coli origin by WB and IP
- Anti-RuvA Antibody (12C6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RuvA Antibody (12C6) for WB applications. These reagents are now offered in bundles with RuvA Antibody (12C6) (see ordering information below).
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RuvA Antibody (12C6) is a mouse monoclonal IgG1 kappa light chain antibody that detects RuvA protein of E. coli origin by western blotting (WB) and immunoprecipitation (IP). Anti-RuvA antibody (12C6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RuvA plays a crucial role in the late stages of homologous recombination and DNA repair, specifically in processing Holliday junctions, which are critical intermediates in these processes. Located in the cytoplasm of Escherichia coli, RuvA binds to both single-stranded and double-stranded DNA, significantly enhancing the ATPase activity of RuvB, especially under UV-irradiation conditions. This interaction promotes branch migration and facilitates the repair of damaged DNA, ensuring genomic stability. The RuvA-RuvB complex is particularly adept at recognizing and interacting with irregular DNA conformations, which is essential for maintaining cellular integrity during replication and repair. Additionally, RuvABC proteins are implicated in the formation of double-strand breaks at stalled replication forks, highlighting RuvA′s importance in cellular responses to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RuvA Antibody (12C6) References:
- Identification and characterization of Thermus thermophilus HB8 RuvA protein, the subunit of the RuvAB protein complex that promotes branch migration of Holliday junctions. | Ohnishi, T., et al. 2000. Genes Genet Syst. 75: 233-43. PMID: 11245216
- A unique beta-hairpin protruding from AAA+ ATPase domain of RuvB motor protein is involved in the interaction with RuvA DNA recognition protein for branch migration of Holliday junctions. | Han, YW., et al. 2001. J Biol Chem. 276: 35024-8. PMID: 11427534
- Holliday junction binding and processing by the RuvA protein of Mycoplasma pneumoniae. | Ingleston, SM., et al. 2002. Eur J Biochem. 269: 1525-33. PMID: 11874468
- Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery. | Yamada, K., et al. 2002. Mol Cell. 10: 671-81. PMID: 12408833
- A tetramer-octamer equilibrium in Mycobacterium leprae and Escherichia coli RuvA by analytical ultracentrifugation. | Lee, YC., et al. 2003. J Mol Biol. 333: 677-82. PMID: 14568529
- The role of RuvA octamerization for RuvAB function in vitro and in vivo. | Privezentzev, CV., et al. 2005. J Biol Chem. 280: 3365-75. PMID: 15556943
- RuvA is a sliding collar that protects Holliday junctions from unwinding while promoting branch migration. | Kaplan, DL. and O'Donnell, M. 2006. J Mol Biol. 355: 473-90. PMID: 16324713
- In vitro reconstitution of the late steps of genetic recombination in E. coli. | Eggleston, AK., et al. 1997. Cell. 89: 607-17. PMID: 9160752
- Formation of RuvABC-Holliday junction complexes in vitro. | Davies, AA. and West, SC. 1998. Curr Biol. 8: 725-7. PMID: 9637927
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RuvA Antibody (12C6) | sc-53435 | 200 µg/ml | $316.00 | |||
RuvA Antibody (12C6): m-IgG Fc BP-HRP Bundle | sc-526808 | 200 µg Ab; 10 µg BP | $354.00 | |||
RuvA Antibody (12C6): m-IgG1 BP-HRP Bundle | sc-532181 | 200 µg Ab; 20 µg BP | $354.00 | |||
RuvA Antibody (12C6) AC | sc-53435 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RuvA Antibody (12C6) HRP | sc-53435 HRP | 200 µg/ml | $316.00 | |||
RuvA Antibody (12C6) FITC | sc-53435 FITC | 200 µg/ml | $330.00 | |||
RuvA Antibody (12C6) PE | sc-53435 PE | 200 µg/ml | $343.00 | |||
RuvA Antibody (12C6) Alexa Fluor® 488 | sc-53435 AF488 | 200 µg/ml | $357.00 | |||
RuvA Antibody (12C6) Alexa Fluor® 546 | sc-53435 AF546 | 200 µg/ml | $357.00 | |||
RuvA Antibody (12C6) Alexa Fluor® 594 | sc-53435 AF594 | 200 µg/ml | $357.00 | |||
RuvA Antibody (12C6) Alexa Fluor® 647 | sc-53435 AF647 | 200 µg/ml | $357.00 | |||
RuvA Antibody (12C6) Alexa Fluor® 680 | sc-53435 AF680 | 200 µg/ml | $357.00 | |||
RuvA Antibody (12C6) Alexa Fluor® 790 | sc-53435 AF790 | 200 µg/ml | $357.00 |