Protein and Antibody Labeling

CruzFluor™ 647 succinimidyl ester is a highly reactive dye that exhibits exceptional specificity in labeling proteins and antibodies through its succinimidyl ester functionality. This compound engages in efficient amine coupling, leading to stable conjugates. Its unique spectral properties allow for precise fluorescence detection, while its hydrophilic characteristics enhance solubility in aqueous environments. The dye's robust photostability ensures reliable performance in diverse experimental conditions, making it ideal for advanced imaging applications.

CruzFluor™ 488 succinimidyl ester is a vibrant fluorescent dye designed for precise protein and antibody labeling. Its succinimidyl ester group facilitates rapid and selective conjugation with primary amines, resulting in stable and highly fluorescent conjugates. The compound's distinct excitation and emission spectra enable clear visualization in complex biological samples. Additionally, its favorable solubility profile enhances compatibility with various buffers, ensuring consistent performance in diverse experimental setups.

FITC-Dextran 70 is a high-molecular-weight fluorescent conjugate that exhibits unique properties for probing cellular environments. Its dextran backbone provides excellent solubility and biocompatibility, while the FITC moiety allows for specific excitation and emission characteristics. This compound interacts with proteins and antibodies through non-covalent interactions, enhancing signal detection in assays. Its size and hydrophilicity facilitate diffusion in biological matrices, making it ideal for studying cellular uptake and distribution.

Coomassie Blue R-250 is a synthetic dye renowned for its ability to bind to proteins, forming stable complexes that exhibit distinct colorimetric changes. This dye interacts primarily through ionic and hydrophobic interactions, allowing for selective visualization of proteins in various assays. Its unique spectral properties enable effective detection and quantification, while its solubility in aqueous solutions enhances its utility in protein analysis. The dye's affinity for aromatic amino acids further contributes to its specificity in protein staining.

Bromophenol Blue is a pH indicator that exhibits unique color transitions, making it useful in visualizing protein interactions. Its anionic structure allows it to bind to positively charged regions on proteins, facilitating the formation of stable complexes. This dye's distinct spectral characteristics enable precise monitoring of protein migration in electrophoresis. Additionally, its solubility in water and sensitivity to pH changes enhance its role in biochemical assays, providing clear visual cues for protein behavior.

5-Carboxyfluorescein-N-hydroxysuccinimide Ester is a fluorescent labeling reagent that exhibits high reactivity towards amine groups on proteins and antibodies. Its ester functionality enables efficient conjugation, resulting in stable fluorescent tags that enhance detection sensitivity. The compound's unique spectral properties allow for clear visualization in various assays, while its hydrophilic nature ensures solubility in aqueous environments, facilitating diverse biochemical applications.

CruzFluor™ 555 succinimidyl ester is a highly reactive fluorescent dye designed for conjugation with proteins and antibodies. Its succinimidyl ester moiety selectively targets primary amines, promoting rapid and efficient labeling. The resulting conjugates exhibit exceptional photostability and brightness, making them ideal for advanced imaging techniques. Additionally, the compound's unique spectral characteristics enable precise multiplexing in complex biological systems, enhancing experimental versatility.

7-Ethoxyresorufin is a versatile chemical compound known for its role in probing enzymatic activity, particularly in cytochrome P450 pathways. Its unique structure allows for specific interactions with target proteins, facilitating the study of metabolic processes. The compound exhibits distinct fluorescence properties, enabling sensitive detection in biochemical assays. Its rapid reaction kinetics enhance the reliability of experimental results, making it a valuable tool in biochemical research.

CruzFluor™ 405 succinimidyl ester is a highly reactive fluorescent dye that forms stable conjugates with proteins and antibodies through amine coupling. Its unique spectral properties allow for precise tracking in complex biological systems. The compound's efficient reaction kinetics enable rapid labeling, while its hydrophilic nature enhances solubility in aqueous environments. This facilitates improved signal intensity and resolution in various analytical applications, making it an essential tool for studying protein interactions.

Rhodamine B isothiocyanate is a vibrant fluorescent dye known for its ability to selectively label proteins and antibodies via thiourea formation. Its unique reactivity with nucleophilic sites on biomolecules allows for robust conjugation, enhancing fluorescence stability. The compound exhibits strong absorbance and emission characteristics, making it ideal for visualizing molecular interactions. Additionally, its hydrophobic properties can influence cellular uptake and localization, providing insights into protein dynamics.