CruzFluor™ 488 succinimidyl ester MW: 1513.82
A fluorophore dye used for labeling proteins, antibodies and nucleic acids.

CruzFluor™ 488 succinimidyl ester

CruzFluor 488 succinimidyl ester is rated 5.0 out of 5 by 2.
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Application: CruzFluor™ 488 succinimidyl ester is a fluorophore dye used for labeling proteins, antibodies and nucleic acids
Molecular Weight: 1513.82
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Cruzfluor™ 488 succinimidyl ester is a superior protein conjugate fluorescein derivative for FITC and comparable replacement for Alexa Fluor® 488 NHS ester, Cy2®, DyLight® 488 and Fluorescein. Cruzfluor™ 488 succinimidyl ester dyes have selectivity for protein amino groups and a respective excitation and emission maxima of 491 nm and 520 nm, and the conjugates are more stable, magnitudes brighter and do not have some of the limitations that are found in FITC. The CruzfluorTM 488 is unaffected by pH 4-10, a marked improvement over a necessary pH above 9 for maximum fluorescence and does not suffer photobleaching for microscope imaging. Additionally, CruzFluor™ 488 (CFL 488) is offered conjugated to commonly used secondary antibodies for use in flow cytometry and immunofluorescence.
The CruzFluorTM family of products are a fluorescent labeling dye series that spans the full visible spectrum, and are useful to conjugate to nucleic acids, proteins and antibodies. The CruzFluorTM dyes are strong reagents for amine modification because they are incorporated with succinimidyl esters, which have a very strong ability to develop amide bonds that are as stable if not identical to the bonds found on a natural peptide.

Physical State :
Solid
Solubility :
Soluble in water (Low), and DMSO.
Storage :
Desiccate at -20° C
Fluoresence :
λex 491 nm, λem 514 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Recommendations for reconstitution?

Asked by: chemicalsmg
Thank you for your question. CruzFluor™ 488 succinimidyl ester, sc-362617, can be reconstituted by adding anhydrous DMSO into the vial to make a 10-20 mM stock solution. Mix well by pipetting or vortex. Extended storage of the dye stock solution may reduce the dye activity. Can be stored in freezer for two weeks when kept from light and moisture. Avoid freeze-thaw cycles.
Answered by: TechService7
Date published: 2016-12-22
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Rated 5 out of 5 by from Product sc Product sc-362617A, CruzFluor 488 succinimidyl ester, is a suitable dye to use for labeling proteins, antibodies and nucleic acids. This product can be used as a suitable alternative to Alexa Fluor 488 NHS ester, Cy2, DyLight 488 and FITC. Conjugates made with this Cruz Fluor dye was found to be more stable and exhibited a higher intensity of fluorescence than these. Stable across a wider range of pH (4-10) and does not photo-bleach. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Product sc Product sc-362617 was conjugated to a primary antibody against SLBP protein. Immunofluorescence staining of HepG2 cells was performed to indicate cytoplasmic localization. The signal observed was bright and crisp. -SCBT QC
Date published: 2015-04-21
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