7-Ethoxyresorufin (CAS 5725-91-7)
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7-Ethoxyresorufin is a compound used in biochemical assays to assess cytochrome P450 enzyme activity, particularly CYP1A1, an enzyme involved in the metabolism of xenobiotics. The compound is a substrate for CYP1A1, and upon metabolism, it is converted to resorufin, a highly fluorescent product that can be easily quantified. This conversion process is the basis for the ethoxyresorufin-O-deethylase (EROD) assay, a widely used method to measure the induction and activity of CYP1A1. The EROD assay, utilizing 7-Ethoxyresorufin, is applied in toxicology to study the effects of environmental and chemical exposures on the induction of detoxifying enzymes. Moreover, the assay is instrumental in the field of ecotoxicology, where it is used to monitor the presence of environmental contaminants that act as inducers of cytochrome P450 enzymes in wildlife. In addition to its use in enzyme activity assays, 7-Ethoxyresorufin is also employed in research focused on understanding the metabolic pathways of xenobiotic compounds and the genetic regulation of these pathways.
7-Ethoxyresorufin (CAS 5725-91-7) References
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- Phe-125 and Phe-226 of pig cytochrome P450 1A2 stabilize the binding of aflatoxin B1 and 7-ethoxyresorufin through the key CH/π interactions. | Zhu, S., et al. 2019. Biochem Pharmacol. 166: 292-299. PMID: 31173723
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
7-Ethoxyresorufin, 2 mg | sc-200606 | 2 mg | $135.00 | |||
7-Ethoxyresorufin, 10 mg | sc-200606A | 10 mg | $265.00 |