Popular Stains, Dyes and Fluorescent Probes

Alcian Blue 8GX is a versatile staining agent renowned for its affinity for acidic polysaccharides and glycosaminoglycans. Its unique cationic nature allows it to form stable complexes with negatively charged cellular components, resulting in vivid blue coloration. The dye's selective binding properties enable precise visualization of tissue structures, while its robust photophysical characteristics ensure reliable fluorescence under various imaging conditions. This makes it an essential tool for histological studies.

FlAsH-EDT2 is a highly selective fluorescent probe that exhibits remarkable affinity for specific protein motifs, particularly those containing tetracysteine sequences. Its unique mechanism involves a covalent bond formation, resulting in a bright fluorescence signal that is sensitive to the local environment. This probe's rapid reaction kinetics and low background fluorescence make it ideal for real-time imaging, enabling precise tracking of protein dynamics and interactions within live cells.

Fluorol Yellow 088 is a vibrant fluorescent dye characterized by its exceptional photostability and high quantum yield. Its unique structure allows for strong π-π stacking interactions, enhancing its binding to various substrates. The dye exhibits a distinct absorption and emission profile, making it ideal for multi-channel imaging. Its rapid diffusion in aqueous environments ensures efficient staining, while its low toxicity allows for prolonged observation in biological systems.

Crystal Violet is a synthetic dye known for its intense coloration and ability to bind to various biological macromolecules, particularly proteins and nucleic acids. Its unique planar structure allows for effective stacking interactions, enhancing its staining capabilities. The dye exhibits notable fluorescence, which is amplified in specific environments, making it useful for imaging applications. Additionally, its stability under diverse conditions contributes to its reliability in various experimental setups.

Congo Red is a versatile dye known for its unique ability to form stable complexes with amyloid proteins, making it a key tool in histological studies. Its distinct molecular structure allows for strong π-π stacking interactions, enhancing its fluorescence under specific conditions. The dye exhibits a bathochromic shift when bound to target molecules, providing vivid color changes that facilitate the identification of structural features in biological samples. Its solubility in water further enhances its utility in various staining protocols.

Lucigenin is a versatile fluorescent probe known for its ability to generate intense luminescence upon interaction with reactive oxygen species. Its unique structure facilitates specific binding to various biomolecules, allowing for the visualization of cellular processes. The probe exhibits distinct photophysical properties, including a high quantum yield and stability under diverse conditions. Additionally, its rapid electron transfer kinetics enable real-time monitoring of redox reactions, making it an effective tool for studying oxidative stress in biological systems.

Sudan Black B is a synthetic dye renowned for its ability to selectively stain lipids and fats, leveraging hydrophobic interactions to bind effectively to these biomolecules. Its unique conjugated structure allows for strong absorbance in the visible spectrum, resulting in a deep black coloration. The dye exhibits notable fluorescence under UV light, making it a valuable tool for visualizing lipid-rich structures. Additionally, its solubility in organic solvents enhances its versatility in various staining protocols.

Ponceau-S, Sodium Salt is a vibrant azo dye characterized by its strong affinity for proteins and polysaccharides, enabling effective staining in various biological assays. Its unique molecular structure allows for specific interactions with amino acid residues, enhancing colorimetric detection. The dye exhibits excellent solubility in water, facilitating uniform distribution in samples. Additionally, its stability under diverse conditions makes it a reliable choice for visualizing complex biological structures.

Fast Blue B Salt is a synthetic dye characterized by its unique ability to form stable complexes with various biomolecules, enhancing its application as a fluorescent probe. Its distinct molecular structure promotes efficient electron transfer, resulting in pronounced fluorescence under specific excitation wavelengths. The compound's high solubility in aqueous solutions and rapid reaction kinetics make it particularly effective for dynamic imaging and tracking in biological systems, showcasing its versatility in research.

Tetramethylrhodamine isothiocyanate mixed isomers are renowned for their exceptional photostability and high quantum yield, making them ideal for fluorescence microscopy and imaging applications. Their unique isothiocyanate functional group facilitates covalent bonding with amino groups in proteins, enabling specific labeling. This selective reactivity enhances the visualization of cellular structures and dynamics, while their vibrant color and broad absorption spectrum allow for versatile multiplexing in complex biological systems.