FlAsH-EDT2 is a fluorescence probe for labeling proteins. The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. Labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT2). The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin. FlAsH-EDT2 binds covalently to a tetracysteine sequence (CCPGCC).
Soluble in chloroform.
Store at -20° C
169-172° C (dec.)
849.8° C at 760 mmHg (Predicted)
λex 508 nm, λem 528 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Can FlAsH-EDT2 be stored in DMSO or DMF and if so for how long at which conditions?
Asked by: Jaro
Thank you for your question. FlAsH-EDT2 (CAS 212118-77-9) is slightly soluble in chloroform and methanol. We recommend storing it in its solid form at -20 °C and preparing working solutions fresh to prevent degradation or precipitation of the solute.
For further questions please contact technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
Is this soluble in DMF?
Asked by: vanesp
Thank you for your question. This is soluble in DMF at 15mg/ml respectively, and should be purged with an inert gas.
Answered by: Technical Service
Date published: 2018-03-06
Has it been tried to target FlAsH-EDT2 with antibodies against fluorescein ? Or more generally, are there antibodies against FlAsH-EDT2 ?
Asked by: TTTT
Thank you for your question. We do not currently offer any antibodies against FlAsH-EDT2. We also are not aware of any publications citing use of the use of this FlAsH-EDT2 (CAS 212118-77-9) flourescent probe with antibodies against flourescein.
Answered by: Technical Support
Date published: 2017-03-06
Is this soluble in DMSO?
Asked by: SCchem16
Thank you for your question. This chemical, FlAsH-EDT2 (CAS 212118-77-9), is only partially soluble in DMSO.
Answered by: Chemical Support 2
Date published: 2017-02-28
This structure differs from ones reported elsewhere (e.g. in the original publication or on: en.wikipedia.org/wiki/FlAsH-EDT2). These other sources report a free carboxylic group on the 3rd carbon whereas you report a lactone. Which is correct?
Asked by: AndrewH
Thank you for your question. Regarding compound FlAsH-EDT2 (CAS 212118-77-9), the structures are just tautomers of each other - they will interconvert, and both are correct.
Rated 5 out of
Various publications cite FlAsHVarious publications cite FlAsH-EDT2 as a fluorescence probe for labeling proteins. The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin. -SCBT Publication Review
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