Date published: 2025-10-16
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Sudan Black B (CAS 4197-25-5) - chemical structure image
Molecular structure of Sudan Black B, CAS Number: 4197-25-5
Sudan Black B (CAS 4197-25-5) - chemical structure image
Molecular structure of Sudan Black B, CAS Number: 4197-25-5
Molecular structure of Sudan Black B, CAS Number: 4197-25-5

Sudan Black B (CAS 4197-25-5)

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Alternate Names:
Sudan Black B is also known as Solvent Black 3, Fat Black HB, and CI 26150
Application:
Sudan Black B is a fat-soluble diazo dye which has been used to stain phospholipids and intracellular lipids
CAS Number:
4197-25-5
Purity:
≥99%
Molecular Weight:
456.54
Molecular Formula:
C29H24N6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Description
Technical Information
Safety Information
SDS & Certificate of Analysis

Sudan Black B is primarily used for staining of neutral triglycerides and lipids. Also a histochemical stain for chromosomes, Golgi apparatus, and leucocyte granules. Sudan Black B has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and it stains blue-black. Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. Sudan Black B is not as lipid-specific as some of the other Sudan dyes and may be useful to stain other materials. Sudan Black B is useful for detecting fats that are contaminated with oil and grease. Sudan black B is used in the study of haematological disorders as it will stain myeloblasts, but not lymphoblasts. Sudan Black B can also stain materials ionically; in this case some background staining is to be expected, and interpretation of sections stained with it must take this into account.


Sudan Black B (CAS 4197-25-5) References

  1. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue.  |  Schnell, SA., et al. 1999. J Histochem Cytochem. 47: 719-30. PMID: 10330448
  2. Characterization of modified low density lipoprotein subfractions by capillary isotachophoresis.  |  Zorn, U., et al. 2001. Electrophoresis. 22: 1143-9. PMID: 11358140
  3. Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM).  |  Baschong, W., et al. 2001. J Histochem Cytochem. 49: 1565-72. PMID: 11724904
  4. Staining histological lung sections with Sudan Black B or Sudan III for automated identification of alveolar epithelial type II cells.  |  Schneider, JP., et al. 2015. Acta Histochem. 117: 675-80. PMID: 26558990
  5. Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds.  |  Qi, L., et al. 2017. Sci Rep. 7: 8361. PMID: 28827657
  6. Waterborne Polyurethane Coatings with Covalently Linked Black Dye Sudan Black B.  |  Wang, T., et al. 2017. Materials (Basel). 10: PMID: 29143785
  7. Sudan black B as a histological stain for polymeric biomaterials embedded in glycol methacrylate.  |  Hoeksma, EA., et al. 1988. Biomaterials. 9: 463-5. PMID: 3224132
  8. (Histo)chemical aspects of Sudan Black B in various solvents.  |  Frederiks, WM., et al. 1981. Acta Histochem Suppl. 24: 259-65. PMID: 6785833
  9. Some aspects of the value of Sudan Black B in lipid histochemistry.  |  Frederiks, WM. 1977. Histochemistry. 54: 27-37. PMID: 72059

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Sudan Black B, 25 g

sc-203760
25 g
$70.00

Hello, What is the shelf life of the powdered product at 4º? I have an unopened bottle from 2018 and I can't find the expiry date. Thank you.

Asked by: Vsharko
Thank you for your question. Please contact our technical service team with the lot number for information about this compound's viability.
Answered by: Tech Support Europe
Date published: 2024-09-30

From where I can purchase Sudan black b in Indore m.p.

Asked by: Ishrat
Thank you for your question. For accurate availability and shipping information regarding this biochemical product, please contact your local distributor.
Answered by: Tech Service
Date published: 2019-02-04

How long is this compound stable at 4° C?

Asked by: two2igm05
Thank you for your question. The stability of the chemical,Sudan Black B (CAS 4197-25-5), is stable per MSDS but we recommend our customers to test the product every year for quality and stability. In addition, the product is warrantied for one year from the date purchased.
Answered by: Technical Support
Date published: 2019-01-17
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Rated 4 out of 5 by from Buen servicioCompre este producto el mes pasado, todo bien, perfecto.
Date published: 2021-02-03
Rated 5 out of 5 by from Various publications cite the use of SudanVarious publications cite the use of Sudan Black B for staining of neutral triglycerides and lipids. It has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and it stains blue-black. -SCBT Publication Review
Date published: 2015-05-16
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Sudan Black B is rated 4.5 out of 5 by 2.
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