Lucigenin (CAS 2315-97-1)
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Lucigenin is a chemiluminescent probe used to indicate the presence of endogenously generated superoxide anion radicals in cells. Upon single-electron reduction of Lucigenin by a radical species the subsequent form of Lucigenin presents an amplified chemiluminescence which is measurable and can be correlated to the respective amount of reactive radical species present in the system. This approach can be used to probe the natural generation of these species as well as the natural functioning of superoxide dismutase and other enzymes responsible for scavenging radicals. In addition to indicating the presence of radicals in cells, Lucigenin shows interaction with redox-active oxidoreductase enzymes. Lucigenin is shown to mediate the reduction of cytochrome C by xanthine oxidase and to increase the rate of NADPH oxidation. These observations indicate that use of Lucigenin for superoxide analysis is precluded in the presence of high levels of NAD(P)H-oxidoreductases.
Lucigenin (CAS 2315-97-1) References
- Lucigenin is a mediator of cytochrome C reduction but not of superoxide production. | Afanas'ev, IB., et al. 1999. Arch Biochem Biophys. 366: 267-74. PMID: 10356292
- Lucigenin chemiluminescence in human seminal plasma. | Ochsendorf, FR., et al. 2001. Free Radic Res. 34: 153-65. PMID: 11264892
- Chemiluminescence of Lucigenin-Allantoin and Its Application for the Detection of Allantoin. | Saqib, M., et al. 2017. Anal Chem. 89: 1863-1869. PMID: 28208242
- Pyridoxal 5'-phosphate assay based on lucigenin chemiluminescence. | Halawa, MI., et al. 2018. Mikrochim Acta. 185: 381. PMID: 30030633
- Chemiluminescence of Lucigenin/Riboflavin and Its Application for Selective and Sensitive Dopamine Detection. | Lan, Y., et al. 2019. Anal Chem. 91: 2135-2139. PMID: 30582677
- A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite nanoparticles for microRNA detection using magnetic separation. | Chang, Z., et al. 2020. Luminescence. 35: 835-844. PMID: 32173991
- Sonochemiluminescence of lucigenin using amines as coreactant: Reactivity and mechanism studies. | Jin, J., et al. 2020. Ultrason Sonochem. 69: 105249. PMID: 32668386
- Lucigenin-pyrogallol chemiluminescence for the multiple detection of pyrogallol, cobalt ion, and tyrosinase. | Mostafa, IM., et al. 2021. J Food Drug Anal. 29: 510-520. PMID: 35696239
- Development of lucigenin-N-hydroxyphthalimide chemiluminescence system and its application to sensitive detection of Co2. | Ji, K., et al. 2022. Spectrochim Acta A Mol Biomol Spectrosc. 279: 121459. PMID: 35700613
- [Chemiluminescent assay for biological substances using lucigenin]. | Maeda, M. and Tsuji, A. 1997. Yakugaku Zasshi. 117: 864-74. PMID: 9414596
- Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems. | Li, Y., et al. 1998. J Biol Chem. 273: 2015-23. PMID: 9442038
- Validation of lucigenin as a chemiluminescent probe to monitor vascular superoxide as well as basal vascular nitric oxide production. | Skatchkov, MP., et al. 1999. Biochem Biophys Res Commun. 254: 319-24. PMID: 9918836
- What do we measure with luminol-, lucigenin- and penicillin-amplified chemiluminescence? 1. Investigations with hydrogen peroxide and sodium hypochlorite. | Rost, M., et al. 1998. J Biolumin Chemilumin. 13: 355-63. PMID: 9926363
- Lucigenin as a substrate of microsomal NAD(P)H-oxidoreductases. | Schepetkin, IA. 1999. Biochemistry (Mosc). 64: 25-32. PMID: 9986909
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lucigenin, 10 mg | sc-202698 | 10 mg | $29.00 | |||
Lucigenin, 50 mg | sc-202698A | 50 mg | $53.00 | |||
Lucigenin, 500 mg | sc-202698B | 500 mg | $175.00 | |||
Lucigenin, 1 g | sc-202698C | 1 g | $302.00 |