Popular Stains, Dyes and Fluorescent Probes

Cresyl Violet acetate is a versatile dye characterized by its affinity for cellular components, particularly nucleic acids and proteins. Its unique ability to form hydrogen bonds and engage in hydrophobic interactions enhances its staining efficacy. The compound exhibits distinct fluorescence properties, allowing for clear visualization under specific light conditions. Additionally, its stability across a range of pH levels makes it suitable for various experimental applications, providing consistent results in complex biological environments.

Alizarin Red S is a synthetic dye renowned for its chelating properties, particularly with calcium ions, which facilitates its use in staining mineralized tissues. Its planar structure allows for effective π-π stacking interactions, enhancing its fluorescence under UV light. The compound exhibits a strong affinity for binding to metal ions, leading to vivid color changes that are useful in various analytical techniques. Its solubility in water and stability across diverse conditions further contribute to its utility in biological and material sciences.

DAB, 50X, is a highly effective fluorescent probe known for its exceptional photostability and sensitivity. Its unique structure allows for specific interactions with target molecules, enhancing signal intensity in imaging applications. The compound exhibits rapid reaction kinetics, enabling swift labeling of biological samples. Its ability to form stable conjugates with proteins and nucleic acids further amplifies its utility in various analytical techniques, making it a preferred choice for researchers.

4-Methylumbelliferyl palmitate is a versatile fluorescent probe known for its ability to undergo hydrolysis, releasing a highly fluorescent 4-methylumbelliferone. This reaction is facilitated by specific esterases, allowing for targeted visualization of enzymatic activity. Its lipophilic nature enhances membrane permeability, making it effective for studying cellular processes. The compound's distinct fluorescence properties enable sensitive detection, providing insights into biochemical pathways and interactions.

CalciFluor™ Fluo-8, AM is a highly effective fluorescent probe known for its selective binding to calcium ions, enabling real-time imaging of cellular calcium dynamics. Its unique design allows for enhanced fluorescence upon calcium binding, resulting in a significant increase in signal intensity. The compound's lipophilic nature facilitates cellular uptake, while its stability under physiological conditions ensures reliable performance in various experimental settings, making it a preferred choice for studying calcium signaling pathways.

Nile Red is a lipophilic dye renowned for its exceptional fluorescence, particularly in non-polar environments. Its unique ability to intercalate into lipid membranes allows for selective staining of lipid-rich structures, making it a powerful tool for visualizing cellular lipid dynamics. The compound exhibits a pronounced shift in emission spectra based on the polarity of its surroundings, enabling sensitive detection of lipid content and distribution in various biological samples.

Coomassie Stain is a widely used dye that exhibits strong binding affinity to proteins through non-covalent interactions, primarily via ionic and hydrophobic forces. This unique binding mechanism results in a vivid color change, allowing for sensitive detection in gel electrophoresis. Its ability to form stable complexes with amino acid residues enhances the visibility of protein bands, making it an essential tool for analyzing protein concentration and purity in various biochemical assays.

4-Methylumbelliferyl α-L-iduronide (free acid) is a versatile fluorescent probe known for its exceptional sensitivity to enzymatic activity. Its unique structure allows for specific interactions with glycosaminoglycans, leading to a pronounced fluorescence upon hydrolysis. This compound exhibits rapid reaction kinetics, making it ideal for real-time monitoring of biochemical processes. Its distinct photophysical properties enable effective visualization in various biological contexts, enhancing its utility in research.

Phalloidin CruzFluor™ 405 Conjugate is a potent fluorescent probe that selectively binds to filamentous actin (F-actin), enhancing visualization of cytoskeletal structures. Its unique conjugation with CruzFluor™ 405 allows for bright, stable fluorescence, facilitating high-resolution imaging. The probe's affinity for actin filaments enables detailed studies of cellular morphology and dynamics, while its photostability ensures reliable results over extended observation periods.

Phalloidin CruzFluor™ 647 Conjugate is a highly effective fluorescent probe that exhibits a strong affinity for filamentous actin (F-actin), enabling precise imaging of cytoskeletal architecture. Its unique CruzFluor™ 647 conjugation provides exceptional brightness and photostability, making it ideal for long-term imaging applications. The probe's selective binding properties allow for detailed exploration of cellular processes, enhancing the understanding of actin dynamics and organization.