CalciFluor Fluo-8, AM CAS: 1345980-40-6
MW: 1046.93
A superior Ca2+ fluorescent probe than traditional Fluo-4, AM.

CalciFluor™ Fluo-8, AM (CAS 1345980-40-6)

CalciFluor Fluo-8, AM | CAS 1345980-40-6 is rated 5.0 out of 5 by 1.
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Application: CalciFluor™ Fluo-8, AM is a superior Ca2+ fluorescent probe than traditional Fluo-4, AM
CAS Number: 1345980-40-6
Molecular Weight: 1046.93
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

CalciFluor™ Fluo-8, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Similarly to Fluo-4, AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability. These are subsequently removed by intracellular esterases allowing Ca2+ detection. However CalciFluor™ Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor™ Fluo-8, AM is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior calcium indicator.

Physical State :
Solid
Solubility :
Soluble in DMSO, and water (low).
Storage :
Desiccate at -20° C
Fluoresence :
λex 494 nm, λem 517 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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CalciFluor Fluo-8, AM (CAS 1345980-40-6) Product Citations

See how others have used CalciFluor Fluo-8, AM (CAS 1345980-40-6). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 10 total

PMID: # 31279969  2019. Free Radic. Biol. Med. 141: 338-347.

PMID: # 28196960  von Hoven, G. et al. 2017. MBio. 8:

PMID: # 26803937  Aguettaz, E. et al. 2016. Cell Calcium. 59: 145-55.

PMID: # 27617280  Aguettaz, E. et al. 2016. Data Brief. 8: 1443-7.

PMID: # 26764221  Hussain, A. et al. 2016. Mol. Carcinog. 55: 2135-2155.

PMID: # 27190250  Wei, C. et al. 2016. Exp. Biol. Med. (Maywood). 241: 1505-15.

PMID: # 27147619  von Hoven, G. et al. 2016. Biochem. J. 473: 1929-40.

PMID: # 26470731  Lisak, D. et al. 2016. Cell Death Differ. 23: 358-68.

PMID: # 26045248  Hu, M. et al. 2015. Archives of biochemistry and biophysics. 579: 55-61.

PMID: # 31661467  J. Clin. Invest.

Citations 1 to 10 of 10 total

How long does the signal last?

Asked by: Nuruddeen
Thank you for your question. Unfortunately, we do not have this information.
Answered by: Technical Support
Date published: 2019-09-27

What is the physical appearance of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, CalciFluor Fluo-8, AM, is a red solid.
Answered by: Chemical Support 1
Date published: 2017-02-23

What is the KD value of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, CalciFluo Fluo-8, AM, has a Ca 2+ KD value of 389 nM.
Answered by: Chemical Support 1
Date published: 2017-02-23

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, CalciFluo Fluo-8, AM, is soluble in 10 mg/ml DMSO.
Answered by: Chemical Support 1
Date published: 2017-02-23
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Rated 5 out of 5 by from Various publications cite CalciFluor Fluo Various publications cite CalciFluor Fluo-8, AM as a superior Ca2+ fluorescent probe than traditional Fluo-4, AM. CalciFluor Fluo-8, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. CalciFluor Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor Fluo-8, AM is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor Fluo-8, AM a superior calcium indicator. -SCBT Publication Review
Date published: 2015-06-23
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