Popular Stains, Dyes and Fluorescent Probes

WST-1 is a versatile chemical that serves as a vital tool in cellular assays, particularly for assessing cell viability and proliferation. Its unique structure allows for selective reduction by mitochondrial dehydrogenases, resulting in a colorimetric change that can be quantitatively measured. This compound exhibits rapid reaction kinetics, enabling real-time monitoring of cellular metabolic activity. Additionally, WST-1's solubility in aqueous solutions enhances its utility in various experimental conditions.

Thioflavine S is a prominent fluorescent dye known for its ability to bind specifically to amyloid fibrils, making it invaluable in studies of protein aggregation. Its unique molecular structure facilitates strong π-π stacking interactions, enhancing fluorescence upon binding. This property allows for sensitive detection of conformational changes in proteins. The dye exhibits high photostability, enabling prolonged imaging without significant loss of signal, making it ideal for dynamic studies in biological systems.

Zinpyr-1 is a versatile fluorescent probe known for its selective binding to zinc ions, which induces a significant fluorescence enhancement. This chelation process is characterized by a rapid response time, making it ideal for real-time monitoring of zinc levels in biological systems. Its unique structural features allow for strong π-π stacking interactions, contributing to its stability and sensitivity. The compound's distinct photophysical properties enable it to serve as an effective tool for studying metal ion dynamics.

Phalloidin CruzFluor™ 555 Conjugate is a potent fluorescent probe that selectively binds to F-actin, providing exceptional visualization of cytoskeletal structures. Its unique conjugation with a fluorescent dye enhances signal intensity, enabling high-resolution imaging. The compound exhibits strong affinity for actin filaments, facilitating detailed studies of cellular dynamics and morphology. Its stability and brightness make it an essential tool for advanced fluorescence microscopy techniques.

Phalloidin CruzFluor™ 633 Conjugate is a highly effective fluorescent probe that specifically targets F-actin, allowing for precise imaging of the cytoskeleton. Its unique conjugation with a red-emitting fluorophore enhances contrast and resolution in fluorescence microscopy. The compound's strong binding affinity to actin filaments ensures reliable staining, while its photostability supports prolonged observation of dynamic cellular processes. This makes it an invaluable asset for detailed structural analysis.

JC-1 iodide is a versatile fluorescent probe known for its ability to selectively accumulate in mitochondria, where it exhibits a unique colorimetric shift from green to red in response to changes in membrane potential. This property allows for the assessment of mitochondrial health and function. Its lipophilic nature facilitates rapid cellular uptake, while its dual-emission characteristics enable real-time monitoring of cellular energy states, making it a powerful tool for studying bioenergetics.

Rhodamine 123 is a highly fluorescent dye renowned for its strong affinity for cellular components, particularly mitochondria, where it enhances fluorescence intensity upon binding. Its unique cationic structure allows for selective localization in negatively charged environments, making it an effective probe for studying membrane dynamics. The dye exhibits rapid photobleaching, which can be leveraged in time-resolved fluorescence studies, providing insights into cellular processes and dynamics.

Ponceau S solution is a vibrant azo dye characterized by its ability to form stable complexes with proteins and other biomolecules, resulting in vivid coloration. Its anionic nature facilitates binding to positively charged sites, enhancing its utility in various staining applications. The dye exhibits distinct spectral properties, allowing for effective visualization under UV light. Additionally, its rapid diffusion through membranes enables efficient labeling, making it a versatile tool in biochemical assays.

FITC-Dextran 70 is a highly versatile fluorescent probe known for its exceptional ability to bind to specific biomolecules through non-covalent interactions, such as hydrogen bonding and hydrophobic effects. Its large molecular size enhances its stability in aqueous environments, while the fluorescein isothiocyanate moiety provides strong fluorescence under UV light. This compound exhibits unique diffusion properties, allowing for effective tracking in cellular studies and dynamic imaging applications.

Phalloidin, Coumarin Labeled is a potent actin-binding probe that selectively labels filamentous actin (F-actin) in cellular structures. Its unique coumarin moiety provides strong fluorescence, enabling visualization of cytoskeletal dynamics. The compound exhibits high affinity for F-actin, stabilizing its polymerized form and preventing depolymerization. This specificity allows for detailed studies of cellular morphology and motility, revealing intricate actin filament networks and their interactions in live cells.