WST-1 CAS: 150849-52-8
MF: C19H11IN5O8S2•Na
MW: 651.35

WST-1 (CAS 150849-52-8)

WST-1 | CAS 150849-52-8 is rated 5.0 out of 5 by 2.
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Alternate Names: WST-1 is also known as 4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene Disulfonate.
Application: WST-1 is a highly sensitive tetrazolium reagent that produces a water-soluble formazan in NADH and cell proliferation assays.
CAS Number: 150849-52-8
Molecular Weight: 651.35
Molecular Formula: C19H11IN5O8S2•Na
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

WST-1 is a light red, highly sensitive tetrazolium reagent that produces a water-soluble formazan (dark red color) in NADH and cell proliferation assays. This is due to cleavage by mitochondrial dehydrogenase. WST-1 formazan l max = 438nm; e= 3.7 x 104.


References

Ishiyama, M. et al.: Chem. Pharm. Bull. 41(6), 1118-1122 (1993)

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (10 mg/ml).
Storage :
Store at 4° C
Melting Point :
>285° C (dec.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
6099081
MDL Number :
MFCD01075079
SMILES :
C1=CC(=CC=C1N2N=C(N=[N+]2C3=CC=C(C=C3)I)C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-])[N+](=O)[O-].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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WST-1 (CAS 150849-52-8) Product Citations

See how others have used WST-1 (CAS 150849-52-8). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 12 total

PMID: # 31150435  2019. PLoS ONE. 14: e0217339.

PMID: # 28640317  Wolf, VG. et al. 2017. Food Funct. 8: 2500-2511.

PMID: # 27329107  Wang, S. et al. 2016. Journal of neuroinflammation. 13: 158.

PMID: # 27485575  Lyukmanova, EN. et al. 2016. Sci Rep. 6: 30698.

PMID: # 25927268  Paracatu, LC. et al. 2015. Food & function. 6: 1818-31.

PMID: # 26320685  Saroni Arwa, P. et al. 2015. Journal of ethnopharmacology. 174: 410-8.

PMID: # 24588269  Gubiani, JR. et al. 2014. Journal of natural products. 77: 668-72.

PMID: # 25254058  Paracatu, LC. et al. 2014. Evid Based Complement Alternat Med. 2014: 793629.

PMID: # 24941338  Nazaré, AC. et al. 2014. Molecules (Basel, Switzerland). 19: 8124-39.

PMID: # 24858094  Chapla, VM. et al. 2014. Molecules (Basel, Switzerland). 19: 6597-608.

Citations 1 to 10 of 12 total

Addition of electron transport reagent is mandatory in WST-1 assay? Can I just use WST-1 reagent (dissolved in HEPES pH 7.4) 500 micromolar/well?

Asked by: aadiani0
Thanks for your question. For cell viability NAD(P)-H tetrazolium based assay the WST-1 must react with an electron coupling reagent like PMS. Please contact our technical service for more information.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

I want to make a stock solution of WST-1( Water soluble tetrazolium). How should I store it after dissolving in water, and how long can I store it in solution?

Asked by: SCM4
We recommend dissolving WST-1 in ddH20 and storing the solution for up to 3 months at 4° C.
Answered by: Tech Service 11
Date published: 2017-03-02
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Rated 5 out of 5 by from GREAT PURCHASE I BOUGHT THE REAGENT FOR USE IN AN ASSAY, I GOT VERY GOOD RESULT
Date published: 2016-12-24
Rated 5 out of 5 by from Various publications cite the use of WST Various publications cite the use of WST-1 in NADH and cell proliferation assays. WST-1 is a light red, highly sensitive tetrazolium reagent that produces a water-soluble formazan (dark red color) in NADH and cell proliferation assays due to cleavage by mitochondrial dehydrogenase. -SCBT Publication Review
Date published: 2015-04-06
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