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WST-1 (CAS 150849-52-8) - chemical structure image
Molecular structure of WST-1, CAS Number: 150849-52-8
WST-1 (CAS 150849-52-8) - chemical structure image
Molecular structure of WST-1, CAS Number: 150849-52-8
Molecular structure of WST-1, CAS Number: 150849-52-8

WST-1 (CAS 150849-52-8)

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Alternate Names:
WST-1 is also known as 4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene Disulfonate.
Application:
WST-1 is a highly sensitive tetrazolium reagent that produces a water-soluble formazan in NADH and cell proliferation assays.
CAS Number:
150849-52-8
Molecular Weight:
651.35
Molecular Formula:
C19H11IN5O8S2•Na
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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SDS & Certificate of Analysis

WST-1 is a light red, highly sensitive tetrazolium reagent that produces a water-soluble formazan (dark red color) in NADH and cell viability assays. This is due to cleavage by mitochondrial dehydrogenase. It is used to evaluate cell proliferation and cytotoxicity in various cell types, including tumor and non-tumor cells. WST-1 works by being reduced to a soluble formazan dye in the presence of an electron carrier, such as NADH or NADPH, which is generated by viable cells. WST-1 formazan l max = 438nm e= 3.7 x 104. It is often used in combination with other assays or markers to obtain more comprehensive information on cell viability and metabolic activity. For example, it can be combined with assays for measuring ATP, LDH, or caspase activity to assess different aspects of cell health and function. Overall, WST-1 is useful for researchers studying cell biology, pharmacology, and toxicology.


WST-1 (CAS 150849-52-8) References

  1. Superoxide produced by activated neutrophils efficiently reduces the tetrazolium salt, WST-1 to produce a soluble formazan: a simple colorimetric assay for measuring respiratory burst activation and for screening anti-inflammatory agents.  |  Tan, AS. and Berridge, MV. 2000. J Immunol Methods. 238: 59-68. PMID: 10758236
  2. Detection of microbial growth on polycyclic aromatic hydrocarbons in microtiter plates by using the respiration indicator WST-1.  |  Johnsen, AR., et al. 2002. Appl Environ Microbiol. 68: 2683-9. PMID: 12039720
  3. A collaborative evaluation of the cytotoxicity of two surfactants by using the human corneal epithelial cell line and the WST-1 test.  |  Huhtala, A., et al. 2003. J Ocul Pharmacol Ther. 19: 11-21. PMID: 12648300
  4. Biological effects of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays.  |  Ranke, J., et al. 2004. Ecotoxicol Environ Saf. 58: 396-404. PMID: 15223265
  5. Evaluation of chondrocyte survival in situ using WST-1 and membrane integrity stains.  |  Jomha, NM., et al. 2007. Cell Tissue Bank. 8: 179-86. PMID: 17094019
  6. WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line.  |  Ngamwongsatit, P., et al. 2008. J Microbiol Methods. 73: 211-5. PMID: 18417231
  7. Osteoblast viability on hydroxyapatite with well-adjusted submicron and micron surface roughness as monitored by the proliferation reagent WST-1.  |  Holthaus, MG., et al. 2013. J Biomater Appl. 27: 791-800. PMID: 22262576
  8. Long term and standard incubations of WST-1 reagent reflect the same inhibitory trend of cell viability in rat airway smooth muscle cells.  |  Yin, LM., et al. 2013. Int J Med Sci. 10: 68-72. PMID: 23289007
  9. Cytotoxicity of alloying elements and experimental titanium alloys by WST-1 and agar overlay tests.  |  Song, YH., et al. 2014. Dent Mater. 30: 977-83. PMID: 24946979
  10. Spectrophotometric Assay for Superoxide Dismutase Based on the Reduction of Highly Water-soluble Tetrazolium Salts by Xanthine-Xanthine Oxidase.  |  Ukeda, H., et al. 1999. Biosci Biotechnol Biochem. 63: 485-8. PMID: 27393255
  11. Assay of superoxide dismutase activity in a plate assay using WST-1.  |  Peskin, AV. and Winterbourn, CC. 2017. Free Radic Biol Med. 103: 188-191. PMID: 28017897
  12. Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures.  |  Lutter, AH., et al. 2017. Clin Hemorheol Microcirc. 67: 327-342. PMID: 28869462
  13. [The new colorimetric assay (WST-1) for cellular growth with normal aging and Alzheimer's disease].  |  Takamatsu, N. 1998. Nihon Ronen Igakkai Zasshi. 35: 535-42. PMID: 9778954
  14. A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye  |  Munetaka ISHIYAMA, Masanobu SHIGA, Kazumi SASAMOTO, Makoto MIZOGUCHI, Pin-gang HE. 1993. Chem. Pharm. Bull. 41: 1118-1122.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

WST-1, 100 mg

sc-213165
100 mg
$312.00

WST-1, 500 mg

sc-213165A
500 mg
$1127.00

Addition of electron transport reagent is mandatory in WST-1 assay? Can I just use WST-1 reagent (dissolved in HEPES pH 7.4) 500 micromolar/well?

Asked by: aadiani0
Thanks for your question. For cell viability NAD(P)-H tetrazolium based assay the WST-1 must react with an electron coupling reagent like PMS. Please contact our technical service for more information.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

I want to make a stock solution of WST-1( Water soluble tetrazolium). How should I store it after dissolving in water, and how long can I store it in solution?

Asked by: SCM4
We recommend dissolving WST-1 in ddH20 and storing the solution for up to 3 months at 4° C.
Answered by: Tech Service 11
Date published: 2017-03-02
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Rated 5 out of 5 by from GREAT PURCHASEI BOUGHT THE REAGENT FOR USE IN AN ASSAY, I GOT VERY GOOD RESULT
Date published: 2016-12-24
Rated 5 out of 5 by from Various publications cite the use of WSTVarious publications cite the use of WST-1 in NADH and cell proliferation assays. WST-1 is a light red, highly sensitive tetrazolium reagent that produces a water-soluble formazan (dark red color) in NADH and cell proliferation assays due to cleavage by mitochondrial dehydrogenase. -SCBT Publication Review
Date published: 2015-04-06
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WST-1 is rated 5.0 out of 5 by 2.
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