WST-1 is a light red, highly sensitive tetrazolium reagent that produces a water-soluble formazan (dark red color) in NADH and cell proliferation assays. This is due to cleavage by mitochondrial dehydrogenase. WST-1 formazan l max = 438nm; e= 3.7 x 104.
Ishiyama, M. et al.: Chem. Pharm. Bull. 41(6), 1118-1122 (1993)
Soluble in water (10 mg/ml).
Store at 4° C
>285° C (dec.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Addition of electron transport reagent is mandatory in WST-1 assay? Can I just use WST-1 reagent (dissolved in HEPES pH 7.4) 500 micromolar/well?
Asked by: aadiani0
Thanks for your question. For cell viability NAD(P)-H tetrazolium based assay the WST-1 must react with an electron coupling reagent like PMS. Please contact our technical service for more information.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29
I want to make a stock solution of WST-1( Water soluble tetrazolium). How should I store it after dissolving in water, and how long can I store it in solution?
Asked by: SCM4
We recommend dissolving WST-1 in ddH20 and storing the solution for up to 3 months at 4° C.
Rated 5 out of
GREAT PURCHASEI BOUGHT THE REAGENT FOR USE IN AN ASSAY, I GOT VERY GOOD RESULT
Date published: 2016-12-24
Rated 5 out of
Various publications cite the use of WSTVarious publications cite the use of WST-1 in NADH and cell proliferation assays. WST-1 is a light red, highly sensitive tetrazolium reagent that produces a water-soluble formazan (dark red color) in NADH and cell proliferation assays due to cleavage by mitochondrial dehydrogenase. -SCBT Publication Review
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