JC-1 iodide CAS: 3520-43-2
MF: C25H27Cl4N4•I
MW: 652.23

JC-1 iodide (CAS 3520-43-2)

JC-1 | CAS 3520-43-2 is rated 5.0 out of 5 by 1.
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Alternate Names: JC-1 iodide is also known as CBIC2(3).
Application: JC-1 iodide is a mitochondrial membrane potential dye and fluorescent probe used to test Pgp activity.
CAS Number: 3520-43-2
Purity: ≥98%
Molecular Weight: 652.23
Molecular Formula: C25H27Cl4N4•I
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

JC-1 is commonly used to determine mitochondrial membrane potential. JC-1 iodide is a cationic carbocyanine dye that is membrane permeable and is often used for flow cytometry and fluorescent microscopy. JC-1 iodide changes color as the membrane potential increases. At higher potentials, JC-1 iodide forms aggregates, which changes the fluorescence emission color from green to red. JC-1 iodide is also known as CBIC2(3), 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-1H-benzimidazolium, monoiodide, and 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO (5 mg/ml), ethanol, methanol, acetone, and DMF.
Storage :
Store at -20° C
Fluoresence :
λex 514 nm, λem 531 nm in methanol
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
5492929
MDL Number :
MFCD00188475
Beilstein Registry :
6110369
SMILES :
CCN1C2=CC(=C(C=C2[N+](=C1/C=C/C=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-]

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Certificate of Analysis

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JC-1 iodide (CAS 3520-43-2) Product Citations

See how others have used JC-1 iodide (CAS 3520-43-2). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 12 total

PMID: # 30982162  Chen, W.|Liu, H.|Wang, T.|Bao, G.|Wang, N.|Li, RC.| et al. 2019. J. Mol. Neurosci.

PMID: # 28795251  2018. Mol. Cell. Biochem. 439: 189-198.

PMID: # 28300624  da Silva, DC. et al. 2017. J. Steroid Biochem. Mol. Biol. 171: 178-186.

PMID: # 28602541  Uzu, M. et al. 2017. J. Pharmacol. Sci. 134: 101-107.

PMID: # 27418140  Shen, K.|Lu, F.|Xie, J.|Wu, M.|Cai, B.|Liu, Y.|Zhang, H.|Tan, H.|Pan, Y.|Xu, H.| et al. 2016. Oncotarget. 7: 50596-50611.

PMID: # 26087109  Piscioneri, A. et al. 2015. Acta Biomater. 24: 297-308.

PMID: # 25703814  Çiğ, B. et al. 2015. Biochim. Biophys. Acta. 1848: 2756-65.

PMID: # 24355563  Koval, AV.|Vlasov, P.|Shichkova, P.|Khunderyakova, S.|Markov, Y.|Panchenko, J.|Volodina, A.|Kondrashov, FA.|Katanaev, VL.| et al. 2014. Biochem. Pharmacol. 87: 571-8.

PMID: # 24681519  Ramos-Mandujano, G. et al. 2014. Stem cell research. 12: 690-702.

PMID: # 31819419  Int J Nanomedicine. 14: 9063-9076.

Citations 1 to 10 of 12 total

jc-1 is soluble in DMSO but precipitates our when diluting in PBS (at 37 C). any suggestions?

Asked by: Bressler
Thank you for your question. Unfortunately we have no data on solubility in PBS, we suggest the following: Soluble in DMSO (5 mg/ml), ethanol, methanol, acetone, and DMF.
Answered by: Technical Support
Date published: 2019-04-13

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This product is soluble in DMSO at 5 mg/ml, but it is also soluble in ethanol, methanol, and acetone. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-28
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Rated 5 out of 5 by from Various publications cite the use of JC Various publications cite the use of JC-1 to determine mitochondrial membrane potential. It is a cationic carbocyanine dye that is membrane permeable and is often used for flow cytometry and fluorescent microscopy. This dye changes color as the membrane potential increases, at higher potentials, JC-1 forms aggregates, which changes the fluorescence emission color from green to red. -SCBT Publication Review
Date published: 2015-04-24
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