ORF19 Inhibitors
Chemical inhibitors of ORF19 function primarily by disrupting the actin cytoskeleton and related cellular processes in which ORF19 plays a crucial role. Wiskostatin targets the N-WASP protein, an essential collaborator with the WASH complex, to which ORF19 belongs. By inhibiting N-WASP, Wiskostatin can hinder actin polymerization, a fundamental process for endosomal sorting that involves ORF19. Similarly, Latrunculin A and Cytochalasin D exert their effects by binding to actin monomers and filaments, respectively. Latrunculin A sequesters G-actin, while Cytochalasin D both prevents the addition of new monomers to F-actin and promotes the disassembly of existing filaments. Consequently, these actions can lead to a reduction in ORF19-mediated regulation of the actin network. CK-666 and Mardepodect disrupt the actin architecture by different mechanisms; CK-666 inhibits the Arp2/3 complex responsible for branching actin polymerization, and Mardepodect prevents VCA domains from activating the Arp2/3 complex. Both inhibitors can therefore impair ORF19's influence on actin structure.
SMIFH2 and ML141 interfere with actin dynamics by targeting proteins that regulate the nucleation and elongation of actin filaments. SMIFH2 disrupts formin-mediated actin assembly, which is crucial for maintaining proper actin structure, a process with which ORF19 is associated. ML141, on the other hand, inhibits the Cdc42 GTPase, a key regulator of actin organization, which indirectly affects ORF19's function within the WASH complex. Blebbistatin and Y-27632 target proteins involved in actin-myosin interactions; Blebbistatin inhibits non-muscle myosin II, and Y-27632 inhibits the Rho-associated protein kinase (ROCK). These inhibitors can impair cellular processes such as cytokinesis and cell motility that are regulated by the WASH complex, thereby affecting ORF19's role in these activities. Additionally, NSC 23766 and SecinH3 mediate their inhibitory actions by targeting GTPases that are integral to actin cytoskeleton organization and endocytic trafficking, respectively. NSC 23766 specifically inhibits Rac1 GTPase activation, while SecinH3 impairs ARF6 activation. Lastly, IPA-3 targets PAK1 kinase, which is involved in actin filament dynamics, and as such, inhibition of PAK1 can indirectly affect ORF19's regulatory functions in actin stabilization and disassembly. Each of these chemicals, through their distinct mechanisms, can lead to a reduction in the functional influence of ORF19 on actin architecture and related cellular processes.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Wiskostatin | 253449-04-6 | sc-204399 sc-204399A sc-204399B sc-204399C | 1 mg 5 mg 25 mg 50 mg | $49.00 $124.00 $441.00 $828.00 | 4 | |
Wiskostatin selectively inhibits the activity of N-WASP, a protein which cooperates with the WASH complex, of which ORF19 is a part. Inhibition of N-WASP by Wiskostatin can lead to reduced actin polymerization, a process vital for endosomal sorting functions that involve ORF19. | ||||||
Latrunculin A, Latrunculia magnifica | 76343-93-6 | sc-202691 sc-202691B | 100 µg 500 µg | $265.00 $815.00 | 36 | |
Latrunculin A binds to monomeric G-actin, preventing its polymerization into F-actin. As ORF19 is implicated in the regulation of actin cytoskeleton dynamics through the WASH complex, inhibition of actin polymerization can be expected to disrupt processes that depend on the function of ORF19. | ||||||
Cytochalasin D | 22144-77-0 | sc-201442 sc-201442A | 1 mg 5 mg | $165.00 $486.00 | 64 | |
Cytochalasin D inhibits actin polymerization and induces depolymerization of actin filaments. Given that ORF19 functions as part of a complex involved in actin filament regulation, disruption of actin dynamics by Cytochalasin D can inhibit the cellular activities mediated by ORF19. | ||||||
CK 666 | 442633-00-3 | sc-361151 sc-361151A | 10 mg 50 mg | $321.00 $1040.00 | 5 | |
CK-666 is an inhibitor of the Arp2/3 complex, which controls actin nucleation in branching polymerization. ORF19, as part of the WASH complex, is involved in actin filament branching, and CK-666's inhibition of the Arp2/3 complex is likely to reduce ORF19's functional influence on actin architecture. | ||||||
SMIFH2 | 340316-62-3 | sc-507273 | 5 mg | $140.00 | ||
SMIFH2 is a formin inhibitor that disrupts formin-mediated actin nucleation and elongation. ORF19's role in the actin cytoskeleton arrangement could be functionally inhibited by SMIFH2's impact on formin activity, which is integral to maintaining proper actin structure and function. | ||||||
ML 141 | 71203-35-5 | sc-362768 sc-362768A | 5 mg 25 mg | $137.00 $512.00 | 7 | |
ML141 is a potent and selective inhibitor of the Cdc42 GTPase, which is known to interact with WASH complex members. By inhibiting Cdc42, ML141 can be expected to disrupt the downstream effects of the WASH complex, thereby inhibiting ORF19 function. | ||||||
(±)-Blebbistatin | 674289-55-5 | sc-203532B sc-203532 sc-203532A sc-203532C sc-203532D | 5 mg 10 mg 25 mg 50 mg 100 mg | $183.00 $313.00 $464.00 $942.00 $1723.00 | 7 | |
Blebbistatin inhibits non-muscle myosin II, which is important for cellular processes such as cytokinesis and cell motility that involve actin-myosin interactions. These processes are regulated by the WASH complex, and therefore, Blebbistatin can inhibit ORF19's role in these cellular activities. | ||||||
Y-27632, free base | 146986-50-7 | sc-3536 sc-3536A | 5 mg 50 mg | $186.00 $707.00 | 88 | |
Y-27632 is an inhibitor of the Rho-associated protein kinase (ROCK), which regulates actin cytoskeleton dynamics. Through its inhibition of ROCK, Y-27632 is likely to disrupt the signaling pathways that ORF19 is involved in, thereby inhibiting its function. | ||||||
NSC 23766 | 733767-34-5 | sc-204823 sc-204823A | 10 mg 50 mg | $151.00 $609.00 | 75 | |
NSC 23766 specifically inhibits the Rac1 GTPase by blocking its interaction with GEFs, thereby preventing its activation. As Rac1 is a regulator of actin cytoskeleton organization, NSC 23766 can disrupt the actin-related functions of the WASH complex, and consequently, ORF19. | ||||||
SecinH3 | 853625-60-2 | sc-203260 | 5 mg | $278.00 | 6 | |
SecinH3 is a cytohesin inhibitor that impairs ARF6 activation, a GTPase involved with actin cytoskeleton and endocytic trafficking. Inhibition of ARF6 by SecinH3 can be expected to disrupt functions of the WASH complex, therefore inhibiting ORF19. | ||||||