Glucose Oxidase Activators
Glucose Oxidase Activators are a suite of chemical compounds that enhance the enzyme's activity through distinct yet complementary mechanisms. The enzyme relies on FAD, a cofactor, for its catalytic action to transfer electrons during glucose oxidation. Its activity is directly related to the presence of its primary substrate, glucose, and oxygen, the latter serving as the ultimate electron acceptor for the reaction to produce gluconolactone and hydrogen peroxide. These reactions are further optimized by the presence of methylene blue and benzoquinone, which act as electron mediators, thereby enhancing the enzyme's turnover. Ferrocene and its derivatives bolster glucose oxidase action by facilitating electron transfer, particularly in biosensor applications, enhancing the enzyme's ability to relay electrical signals upon glucose oxidation.
The stability and operational longevity of glucose oxidase are augmented by compounds such as catalase, which eliminates the inhibitory hydrogen peroxide by-product, and non-ionic detergents like Triton X-100 that prevent enzyme aggregation. Additionally, PEGylation with compounds like Polyethylene Glycol enhances stability and solubility, essential for maintaining the enzyme's activity and prolonging its half-life in various applications. Caffeine contributes to this stability by preserving the enzyme's active conformation. Lastly, imidazole ensures an optimal pH, thereby sustaining an environment conducive for peak enzymatic activity.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
D(+)Glucose, Anhydrous | 50-99-7 | sc-211203 sc-211203B sc-211203A | 250 g 5 kg 1 kg | $38.00 $198.00 $65.00 | 5 | |
As the substrate for glucose oxidase, glucose directly increases the enzyme's functional activity by binding to the active site and undergoing oxidation, which is the primary reaction catalyzed by the enzyme. | ||||||
Methylene blue | 61-73-4 | sc-215381B sc-215381 sc-215381A | 25 g 100 g 500 g | $43.00 $104.00 $328.00 | 3 | |
As an artificial electron acceptor, methylene blue can be reduced by glucose oxidase, which in turn can be re-oxidized by oxygen, facilitating the continuous turnover of the enzyme and enhancing its activity. | ||||||
L-Ascorbic acid, free acid | 50-81-7 | sc-202686 | 100 g | $46.00 | 5 | |
Ascorbic acid can reduce back the oxidized FAD in glucose oxidase, which helps in maintaining the enzyme in its active form, thus indirectly enhancing its activity. | ||||||
Triton X-100 | 9002-93-1 | sc-29112 sc-29112A | 100 ml 500 ml | $20.00 $42.00 | 55 | |
Non-ionic detergents like Triton X-100 can increase the solubility and stability of glucose oxidase, enhancing its activity by preventing enzyme aggregation and inactivation. | ||||||
Imidazole | 288-32-4 | sc-204776 sc-204776A sc-204776B sc-204776C | 25 g 100 g 1 kg 5 kg | $27.00 $56.00 $84.00 $343.00 | 2 | |
Imidazole can act as a stabilizing agent for glucose oxidase by buffering the pH, as the enzyme has an optimal activity at acidic pH levels, thereby enhancing its catalytic function. | ||||||
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 50 g 100 g 250 g 1 kg 5 kg | $33.00 $67.00 $97.00 $192.00 $775.00 | 13 | |
Caffeine is reported to stabilize the quaternary structure of glucose oxidase and can enhance its activity by maintaining the enzyme in its active conformation. | ||||||