Methylene blue CAS: 61-73-4
MF: C16H18N3S•Cl
MW: 319.85
A polyaromatic stain.

Methylene blue (CAS 61-73-4)

Methylene blue | CAS 61-73-4 is rated 5.0 out of 5 by 1.
  • y_2020, m_5, d_24, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_215381, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT
5
1
4
0
3
0
2
0
1
0
Alternate Names: Basic Blue 9
Application: Methylene blue is a polyaromatic stain
CAS Number: 61-73-4
Molecular Weight: 319.85
Molecular Formula: C16H18N3S•Cl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Methylene Blue is a sensitive stain used for RNA and ribonuclease. This chemical helps (in vitro) with cyanide poisoning. Additionally it helps in vitro with lowering the levels of methemoglobin. It also in vitro inhibits guanylate cyclase.

Appearance :
Powder
Physical State :
Solid
Storage :
Store at room temperature
Melting Point :
~180° C (dec.)
IC50 :
Plasmodium falciparum: IC50 = 5.4 nM; His-tagged human recombinant 2N4R tau protein aggregation: IC50 = 0.61 µM; Glutathione reductase: IC50 = 6.4 µM (Plasmodium falciparum 3D7); Apaf-1: IC50 = 15.5 µM (human); Glutathione reductase: IC50 = 16 µM (human)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
SO5600000
PubChem CID :
6099
Merck Index :
14: 6060
MDL Number :
MFCD00150006
EC Number :
200-515-2
Beilstein Registry :
3599847
SMILES :
CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.[Cl-]

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Methylene blue (CAS 61-73-4) Product Citations

See how others have used Methylene blue (CAS 61-73-4). Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 31624092  RNA. 26: 19-28.

Citations 1 to 1 of 1 total

Is this pharmaceutical grade, what are the list of impurities and levels, does it contain toxic/obnoxious metals

Asked by: Farhad2
Thank you for your question. This is not pharmaceutical grade. This is research grade. Please contact our Technical Service Department to request a sample Certificate of Analysis (CoA) from a recent lot to see what metals or impurities may be present.
Answered by: Technical Support
Date published: 2020-04-13

How can I prepare methylene blue solution for simple staining ??

Asked by: Eman93
Thank you for your question. This methylene blue biochemical is soluble in water. Here is a link to a Certificate of Analysis for this product: http://datasheets.scbt.com/coa/coa.php?sc=sc-215381&lot=F0719
Answered by: Tech Service
Date published: 2019-10-16
  • y_2020, m_5, d_25, h_14CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_215381, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 90ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Various publications cite the use of the Various publications cite the use of the polyaromatic stain, Methylene blue, for the detection of RNA and ribonuclease. it is also a well-known guanylate cyclase inhibitor. -SCBT Publication Review
Date published: 2015-05-19
  • y_2020, m_5, d_24, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_215381, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 23ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.