Date published: 2025-10-12

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Methylene blue (CAS 61-73-4)

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Alternate Names:
Basic Blue 9
Application:
Methylene blue is a polyaromatic stain
CAS Number:
61-73-4
Molecular Weight:
319.85
Molecular Formula:
C16H18N3S•Cl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Methylene Blue is a sensitive stain used for RNA and ribonuclease. This chemical helps (in vitro) with cyanide poisoning. Additionally it helps in vitro with lowering the levels of methemoglobin. It also in vitro inhibits guanylate cyclase.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Methylene blue, 25 g

sc-215381B
25 g
$42.00

Methylene blue, 100 g

sc-215381
100 g
$102.00

Methylene blue, 500 g

sc-215381A
500 g
$322.00

Is this product pharacutal grade?

Asked by: Dickdee
Thank you for your question. All of our products are of technical grade quality and intended exclusively for research applications. They are not pharmaceutical grade.
Answered by: Tech Support Europe
Date published: 2023-05-27

Is this pharmaceutical grade?

Asked by: Dickdee
Our chemicals are research grade.
Answered by: Technical Service
Date published: 2023-05-27

Is this pharmaceutical grade, what are the list of impurities and levels, does it contain toxic/obnoxious metals

Asked by: Farhad2
Thank you for your question. This is not pharmaceutical grade. This is research grade. Please contact our Technical Service Department to request a sample Certificate of Analysis (CoA) from a recent lot to see what metals or impurities may be present.
Answered by: Technical Support
Date published: 2023-09-14

How can I prepare methylene blue solution for simple staining ??

Asked by: Eman93
Thank you for your question. This methylene blue biochemical is soluble in water. Here is a link to a Certificate of Analysis for this product: http://datasheets.scbt.com/coa/coa.php?sc=sc-215381&lot=F0719
Answered by: Tech Service
Date published: 2023-05-27
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Rated 5 out of 5 by from Various publications cite the use of theVarious publications cite the use of the polyaromatic stain, Methylene blue, for the detection of RNA and ribonuclease. it is also a well-known guanylate cyclase inhibitor. -SCBT Publication Review
Date published: 2015-05-19
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Methylene blue is rated 5.0 out of 5 by 1.
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