FITC Activators
The chemical class of FITC Enhancers refers to a range of compounds that can enhance the fluorescence intensity, stability, or labeling efficiency of FITC (Fluorescein isothiocyanate)-conjugated molecules. FITC is widely used in biological sciences for labeling proteins, antibodies, and other biomolecules for fluorescence-based detection methods. The effectiveness of FITC labeling and the subsequent fluorescence signal can be significantly influenced by the chemical environment and the stability of the labeled compounds.
Compounds such as sodium bicarbonate and Tris buffer are used to maintain an optimal pH, which is crucial for the maximum fluorescence of FITC. Stabilizing agents like glycerol, sucrose, and propylene glycol are essential to protect FITC-labeled molecules from photobleaching, thereby enhancing the durability of the fluorescence signal. Solvents like N,N-Dimethylformamide and ethanol facilitate the solubilization and efficient conjugation of FITC to biomolecules. Sodium azide serves as a preservative, preventing microbial growth in FITC-labeled solutions, thus maintaining the integrity of the fluorescence label.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Sodium bicarbonate | 144-55-8 | sc-203271 sc-203271A sc-203271B sc-203271C sc-203271D | 25 g 500 g 1 kg 5 kg 25 kg | $20.00 $28.00 $42.00 $82.00 $683.00 | 1 | |
Increases the pH, which can enhance the fluorescence of FITC as it fluoresces more intensely in alkaline conditions. | ||||||
Glycerol | 56-81-5 | sc-29095A sc-29095 | 100 ml 1 L | $55.00 $150.00 | 12 | |
Used as a stabilizing agent in many fluorescence applications, glycerol can protect FITC-labeled compounds from photobleaching. | ||||||
Sodium azide | 26628-22-8 | sc-208393 sc-208393B sc-208393C sc-208393D sc-208393A | 25 g 250 g 1 kg 2.5 kg 100 g | $42.00 $152.00 $385.00 $845.00 $88.00 | 8 | |
Used as a preservative in FITC-labeled antibody solutions to prevent microbial growth, thus maintaining the stability of the fluorescence label. | ||||||
Tween-20 | 9005-64-5 | sc-29113C sc-29113 sc-29113B sc-29113A | 100 ml 500 ml 1 L 3.8 L | $18.00 $30.00 $50.00 $160.00 | 54 | |
A detergent that can help in solubilizing FITC-labeled proteins, enhancing the efficiency of labeling. | ||||||
Sucrose | 57-50-1 | sc-204311 sc-204311B sc-204311C sc-204311A | 0.5 kg 50 kg 100 kg 5 kg | $57.00 $1224.00 $1760.00 $195.00 | 6 | |
Acts as a stabilizer in FITC-labeled preparations, protecting against photobleaching. | ||||||