F730047E07Rik Activators
MMS22-like Activators enhance the functional activity of MMS22-like, a protein crucial for the stabilization of the repair complex during homologous recombination (HR), a pathway responsible for the high-fidelity repair of double-strand DNA breaks. The use of PARP inhibitors such as Olaparib, Rucaparib, and PJ34 results in an increase in double-strand DNA breaks due to the prevention of single-strand DNA break repair. This accumulation of DNA damage elevates the need for HR mechanisms, thus enhancing the activity of MMS22-like in the stabilization of the repair complex. Similarly, DNA-PKcs and ATM inhibitors, represented by NU7441 and KU-55933, compromise the efficiency of the non-homologous end joining (NHEJ) repair pathway and the cellular response to DNA damage, respectively. As a consequence, cells become more dependent on HR, where MMS22-like facilitates the processing of repair intermediates and the stabilization of the repair complex.
The inhibition of checkpoint kinases such as ATR and CHK1 with compounds like VE-821 and AZD7762 disrupts proper DNA damage checkpoint activation, increasing the dependency on HR and the function of MMS22-like during replication stress and the maintenance of genomic integrity. The RAD51 inhibitor B02 directly impedes a key protein in homologous recombination, which paradoxically increases the need for other HR proteins, including MMS22-like, to compensate for the impaired RAD51 function. The WEE1 kinase inhibitor MK-1775 forces cells into mitosis with unresolved DNA damage, thus requiring greater MMS22-like activity to facilitate repair before cell division. Caffeine and Nocodazole, although not direct inhibitors of DNA repair, create cellular conditions that intensify the requirement for HR and MMS22-like. Caffeine by indirectly reducing ATM and ATR activities, and Nocodazole by causing cell cycle arrest and an increase in DNA breaks during mitosis, both enhance the need for MMS22-like function to maintain genomic stability.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Olaparib | 763113-22-0 | sc-302017 sc-302017A sc-302017B | 250 mg 500 mg 1 g | $210.00 $305.00 $495.00 | 10 | |
Olaparib is a PARP inhibitor that prevents the repair of single-strand DNA breaks, leading to the accumulation of double-strand breaks and increased engagement of homologous recombination repair pathways, where MMS22-like plays a critical role in stabilizing the repair complex. | ||||||
NU 7441 | 503468-95-9 | sc-208107 | 5 mg | $357.00 | 10 | |
NU7441 is a DNA-PKcs inhibitor that impairs non-homologous end joining (NHEJ), shifting the cellular reliance towards homologous recombination for DNA repair, thus enhancing the functional activity of MMS22-like in the repair process. | ||||||
VE 821 | 1232410-49-9 | sc-475878 | 10 mg | $360.00 | ||
VE-821 is an ATR inhibitor that leads to impaired DNA damage checkpoint activation, resulting in increased reliance on DNA repair proteins like MMS22-like for the resolution of DNA lesions during replication stress. | ||||||
ATM Kinase Inhibitor | 587871-26-9 | sc-202963 | 2 mg | $110.00 | 28 | |
KU-55933 is an ATM inhibitor that reduces the cellular capacity to respond to DNA damage, thereby increasing the dependency on homologous recombination and subsequently the activity of MMS22-like in DNA repair. | ||||||
Rucaparib | 283173-50-2 | sc-507419 | 5 mg | $150.00 | ||
Rucaparib is a PARP inhibitor, like Olaparib, and it increases the burden of double-strand DNA breaks, enhancing the involvement of MMS22-like in homologous recombination repair pathways. | ||||||
AZD7762 | 860352-01-8 | sc-364423 | 2 mg | $107.00 | ||
AZD7762 is a CHK1 inhibitor that leads to the disruption of DNA damage checkpoints, necessitating the increased activity of homologous recombination proteins like MMS22-like to maintain genomic integrity. | ||||||
PARP Inhibitor VIII, PJ34 | 344458-15-7 | sc-204161 sc-204161A | 1 mg 5 mg | $58.00 $142.00 | 20 | |
PJ34 is another PARP inhibitor that, by preventing the repair of single-strand breaks, indirectly necessitates the enhanced activity of homologous recombination repair mechanisms, thus enhancing the function of MMS22-like. | ||||||
RAD51 Inhibitor B02 | 1290541-46-6 | sc-507533 | 10 mg | $95.00 | ||
B02 is a RAD51 inhibitor that impedes RAD51-mediated strand invasion, a step in homologous recombination, increasing the requirement for supporting factors like MMS22-like to stabilize the repair complex. | ||||||
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 50 g 100 g 250 g 1 kg 5 kg | $33.00 $67.00 $97.00 $192.00 $775.00 | 13 | |
Caffeine indirectly impairs ATM and ATR kinase activities, which are important for the DNA damage response, thereby increasing the need for homologous recombination and the activity of proteins like MMS22-like in the repair process. | ||||||
Nocodazole | 31430-18-9 | sc-3518B sc-3518 sc-3518C sc-3518A | 5 mg 10 mg 25 mg 50 mg | $59.00 $85.00 $143.00 $247.00 | 38 | |
Nocodazole disrupts microtubule polymerization, leading to cell cycle arrest and increased double-strand DNA breaks during mitosis, which enhances the requirement for MMS22-like in DNA repair to ensure proper chromosomal segregation. | ||||||