Date published: 2025-11-24

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DNase I Inhibitors

DNase I inhibitors play a pivotal role in the intricate regulation of cellular processes associated with safeguarding DNA integrity. Actinomycin D and Ethidium Bromide stand out as prominent direct inhibitors, as they impede the enzymatic activity of DNase I by engaging with DNA and disrupting its cleavage. Actinomycin D, a potent antitumor antibiotic, intercalates into DNA, inhibiting both transcription and DNase I activity. Similarly, Ethidium Bromide intercalates into DNA strands, preventing DNase I from accessing its substrate. In contrast, ZnSO4, EGTA, and Neocuproine function as indirect inhibitors by modulating pathways linked to DNA integrity. ZnSO4 and neocuproine exert their influence through chelation of zinc and copper ions, respectively, impacting DNase I activity indirectly. EGTA disrupts calcium-dependent pathways associated with DNase I, further contributing to the regulation of DNA integrity. Expanding the spectrum of DNase I inhibitors, Pentoxifylline, DPI, Sodium Butyrate, 3-Aminobenzamide, Trolox, α-Amanitin, and Deferoxamine operate as indirect inhibitors, targeting diverse cellular signaling pathways. Pentoxifylline, recognized for its anti-inflammatory properties, modulates pathways linked to inflammation, indirectly influencing DNase I activity. DPI, a potent inhibitor of NADPH oxidases, intervenes in redox signaling, thereby impacting the delicate balance of cellular processes. Sodium butyrate, on the other hand, alters cellular dynamics through histone acetylation modulation, affecting DNase I indirectly. Additionally, 3-Aminobenzamide inhibits poly(ADP-ribose) polymerase (PARP), contributing to the maintenance of DNA integrity. Trolox, α-Amanitin, and Deferoxamine exert their effects on oxidative stress pathways, providing a multifaceted approach to preserving DNA integrity in diverse biological contexts. In essence, the comprehensive array of DNase I inhibitors, spanning both direct and indirect mechanisms, not only sheds light on the intricate regulatory networks governing cellular processes but also offers invaluable tools for manipulating these processes in various biological scenarios. Their nuanced interactions with DNA-related pathways underscore their significance in ensuring the fidelity of DNA integrity, a critical aspect in the broader landscape of cellular function and homeostasis.

Items 1 to 10 of 11 total

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Product NameCAS #Catalog #QUANTITYPriceCitationsRATING

Actinomycin D

50-76-0sc-200906
sc-200906A
sc-200906B
sc-200906C
sc-200906D
5 mg
25 mg
100 mg
1 g
10 g
$73.00
$238.00
$717.00
$2522.00
$21420.00
53
(3)

Actinomycin D is a direct inhibitor of DNase I, acting by binding to DNA and preventing its degradation by DNase I. This direct inhibition prevents the enzymatic activity of DNase I, thereby maintaining DNA integrity in the cellular context.

Ethidium bromide

1239-45-8sc-203735
sc-203735A
sc-203735B
sc-203735C
1 g
5 g
25 g
100 g
$47.00
$147.00
$576.00
$2045.00
12
(1)

Ethidium Bromide is a direct inhibitor of DNase I by intercalating into DNA, preventing its cleavage by the enzyme. This direct inhibition interferes with the ability of DNase I to degrade DNA, preserving the stability of genetic material in cellular processes.

Zinc

7440-66-6sc-213177
100 g
$47.00
(0)

Zinc sulfate is an indirect inhibitor of DNase I, as zinc ions influence cellular pathways related to DNA integrity. Zinc ions can modulate the activity of nucleases indirectly by affecting cellular processes, potentially leading to a reduction in the activity of DNase I and preserving DNA integrity.

EGTA

67-42-5sc-3593
sc-3593A
sc-3593B
sc-3593C
sc-3593D
1 g
10 g
100 g
250 g
1 kg
$20.00
$62.00
$116.00
$246.00
$799.00
23
(1)

EGTA is a calcium chelator that indirectly inhibits DNase I by disrupting the calcium-dependent pathways associated with its activity. This indirect modulation impacts the enzymatic activity of DNase I, potentially leading to the preservation of DNA integrity in cellular processes.

Pentoxifylline

6493-05-6sc-203184
1 g
$20.00
3
(1)

Pentoxifylline is an indirect inhibitor of DNase I through its modulation of cellular signaling pathways. It can influence pathways related to inflammation, potentially impacting DNase I activity indirectly and preserving DNA integrity in the cellular context.

Sodium Butyrate

156-54-7sc-202341
sc-202341B
sc-202341A
sc-202341C
250 mg
5 g
25 g
500 g
$30.00
$46.00
$82.00
$218.00
19
(3)

Sodium Butyrate is an indirect inhibitor of DNase I through its modulation of histone acetylation and gene expression. This indirect modulation can impact the expression of DNase I and potentially influence its activity, preserving DNA integrity in cellular processes.

3-Aminobenzamide

3544-24-9sc-3501
sc-3501B
sc-3501A
100 mg
1 g
5 g
$15.00
$36.00
$51.00
18
(1)

3-Aminobenzamide is an indirect inhibitor of DNase I through its action as a poly(ADP-ribose) polymerase (PARP) inhibitor. This indirect modulation can influence cellular processes related to DNA repair and potentially impact the activity of DNase I, preserving DNA integrity in the cellular context.

α-Amanitin

23109-05-9sc-202440
sc-202440A
1 mg
5 mg
$260.00
$1029.00
26
(2)

α-Amanitin is a direct inhibitor of RNA polymerase II, indirectly influencing DNase I activity by modulating transcription. This indirect modulation can impact the expression of DNase I and potentially influence its activity, preserving DNA integrity in cellular processes.

Trolox

53188-07-1sc-200810
sc-200810A
sc-200810B
sc-200810C
sc-200810D
500 mg
1 g
5 g
25 g
100 g
$37.00
$66.00
$230.00
$665.00
$1678.00
39
(1)

Trolox is an indirect inhibitor of DNase I through its role as a vitamin E analog, influencing oxidative stress pathways. This indirect modulation can impact DNase I activity, potentially preserving DNA integrity by preventing excessive oxidative damage in cellular processes.

Deferoxamine

70-51-9sc-507390
5 mg
$250.00
(0)

Deferoxamine is an indirect inhibitor of DNase I by chelating iron ions, influencing cellular iron homeostasis. This indirect modulation can impact pathways related to oxidative stress and DNase I activity, potentially preserving DNA integrity in cellular processes.