Molecular structure of Ethidium bromide, CAS Number: 1239-45-8
Ethidium bromide (CAS 1239-45-8)
See product citations (12)
Alternate Names:
EtBr; Dromilac; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Application:
Ethidium bromide is an intercalating fluorescent dye reagent for DNA/RNA staining
CAS Number:
1239-45-8
Molecular Weight:
394.3
Molecular Formula:
C21H20N3•Br
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Ethidium bromide is a polyaromatic fluorescent small molecule and nucleic acid intercalator widely used as a stain in DNA and RNA electrophoresis. Upon intercalation into the hydrophobic region between nucleic acid base pairs, fluorescence-quenching water molecules associated to the Ethidium bromide structure are displaced and the bound Ethidium bromide then fluoresces very strongly compared to free Ethidium bromide (~25 fold increase in dsDNA). Intercalation distorts the ribonucleic acid structure and impedes natural DNA interactions, presenting a powerful mutagen effect associated with Ethidium bromide. This imposition on DNA structure also makes Ethidium bromide an inhibitor of DNA polymerase.
Ethidium bromide (CAS 1239-45-8) References
- The fluorometric determination of nucleic acids in pea seeds by use of ethidium bromide complexes. | El Hamalawi, AR., et al. 1975. Anal Biochem. 67: 384-91. PMID: 1163763
- [INTERACTION OF ETHIDIUM BROMHYDRATE (BET) WITH NUCLEIC ACIDS (NA). SPECTROFLUORIMETRIC STUDY]. | LEPECQ, JB., et al. 1964. C R Hebd Seances Acad Sci. 259: 1786-9. PMID: 14196160
- The effects of antimicrobial agents on deoxyribonucleic acid polymerase. | Elliott, WH. 1963. Biochem J. 86: 562-7. PMID: 16748992
- Binding of ethidium bromide to a DNA triple helix. Evidence for intercalation. | Scaria, PV. and Shafer, RH. 1991. J Biol Chem. 266: 5417-23. PMID: 2005088
- A densitometric nondestructive microassay for DNA quantitation. | Moore, SP. and Sutherland, BM. 1985. Anal Biochem. 144: 15-9. PMID: 2858989
- Ethidium Bromide Modifies The Agarose Electrophoretic Mobility of CAG•CTG Alternative DNA Structures Generated by PCR. | Gomes-Pereira, M. and Monckton, DG. 2017. Front Cell Neurosci. 11: 153. PMID: 28611596
- Mechanistic insights into ethidium bromide removal by palygorskite from contaminated water. | Chang, PH. and Sarkar, B. 2021. J Environ Manage. 278: 111586. PMID: 33171377
- Binding of ethidium bromide to double-stranded ribonucleic acid. | Douthart, RJ., et al. 1973. Biochemistry. 12: 214-20. PMID: 4630220
- A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. | LePecq, JB. and Paoletti, C. 1967. J Mol Biol. 27: 87-106. PMID: 6033613
- A DNA-binding protein from E. coli isolation, characterization and its relationship with proteins H1 and B1. | Laine, B., et al. 1984. Biochem Biophys Res Commun. 119: 1147-53. PMID: 6370250
- Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates. | Karsten, U. and Wollenberger, A. 1977. Anal Biochem. 77: 464-70. PMID: 842829
Inhibitor of:
ANT4, anti-Cy5, ATP6, C11orf83, C14orf106, C14orf135, C3orf60, DDX10, DDX19A, DDX19B, DDX35, DNA Polymerase, ds DNA Marker, EF-G2, EG546368, GTPBP10, GTPBP8, hCG_38480, HMG-20A, I-SceI, LOC255130, MMAA, MOG, MOV10L1, MRP-L54, MRPL28, MTFmt, MTG1, PTCD2, RNA Polymerase, RNase T2, Rpp14, Rpp20, RPUSD2, SURF-1, TaR-1, TFB2M, and UQCRB.Activator of:
Ape2, HcRed, hDcp1b, HELIC2, HMG-2, LONP2, MRRF, MTERF, NAP1, POL H, Poliovirus Type 2, RecA, Ribosomal Protein L17, RNA pol α, rRNA, RuvC, ss DNA marker, SSB-1, TaR-1, and TDP1.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ethidium bromide, 1 g | sc-203735 | 1 g | $47.00 | |||
Ethidium bromide, 5 g | sc-203735A | 5 g | $147.00 | |||
Ethidium bromide, 25 g | sc-203735B | 25 g | $576.00 | |||
Ethidium bromide, 100 g | sc-203735C | 100 g | $2045.00 |