DNA pol ζ Activators
DNA polymerase ζ (Pol ζ) activators are a class of chemical compounds known for their ability to influence and promote the activity of DNA polymerase ζ, a specialized DNA polymerase enzyme involved in DNA replication and repair processes. These activators function by interacting with the cellular machinery responsible for DNA synthesis and damage repair, facilitating the recruitment and activity of Pol ζ in various DNA transactions. Pol ζ, a member of the Y-family DNA polymerases, plays a crucial role in replicating and repairing damaged DNA, particularly in situations where the DNA template contains lesions or structural abnormalities. The mechanism of action of DNA Pol ζ activators primarily involves enhancing the efficiency of translesion synthesis (TLS), a specialized DNA repair process. During TLS, Pol ζ is recruited to sites of DNA damage or lesions where the conventional DNA polymerases encounter roadblocks. Pol ζ's unique ability to insert nucleotides opposite damaged bases allows it to bypass these obstacles and continue DNA synthesis.
DNA Pol ζ activators can promote this process by either directly binding to the Pol ζ complex or modulating the cellular factors that regulate its recruitment and activity. By facilitating Pol ζ-mediated TLS, these activators contribute to the maintenance of genomic integrity by ensuring accurate DNA replication and repair, even in the presence of challenging DNA lesions. In summary, DNA Pol ζ activators form a class of chemicals that enhance the function of DNA polymerase ζ, a vital enzyme in DNA replication and repair processes. They play a crucial role in facilitating the bypass of DNA lesions and ensuring the accuracy of DNA synthesis in challenging genomic environments. Understanding the mechanisms and properties of these activators is essential for advancing our knowledge of DNA repair mechanisms and their impact on genomic stability.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Methyl methanesulfonate | 66-27-3 | sc-250376 sc-250376A | 5 g 25 g | $56.00 $133.00 | 2 | |
MMS is an alkylating agent that adds methyl groups to DNA bases, primarily guanine. This alkylation leads to the formation of DNA lesions that can result in base mispairing. Pol ζ is recruited to aid in the repair of these lesions by promoting translesion synthesis (TLS). It helps insert the correct nucleotides across from the damaged bases. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $138.00 $380.00 | 101 | |
Cisplatin forms intrastrand and interstrand crosslinks in DNA, leading to distortions in the double helix. Pol ζ is involved in TLS to bypass these crosslinks and facilitate repair. | ||||||
Mitomycin C | 50-07-7 | sc-3514A sc-3514 sc-3514B | 2 mg 5 mg 10 mg | $66.00 $101.00 $143.00 | 85 | |
Mitomycin C generates DNA crosslinks between complementary DNA strands. Pol ζ is recruited to aid in TLS and repair of the crosslinks, preventing replication fork stalling. | ||||||
Hydrogen Peroxide | 7722-84-1 | sc-203336 sc-203336A sc-203336B | 100 ml 500 ml 3.8 L | $31.00 $61.00 $95.00 | 28 | |
H2O2 generates reactive oxygen species (ROS) within cells, leading to oxidative DNA damage, including base modifications and single-strand breaks. Pol ζ participates in TLS to manage and repair these oxidative lesions. | ||||||
4-Nitroquinoline N-oxide | 56-57-5 | sc-256815 sc-256815A | 1 g 5 g | $124.00 $421.00 | 6 | |
4-NQO is a genotoxic compound that induces various DNA lesions, including DNA adducts and mutations. Pol ζ is activated to handle these DNA lesions, ensuring accurate DNA repair and replication. | ||||||
Temozolomide | 85622-93-1 | sc-203292 sc-203292A | 25 mg 100 mg | $91.00 $255.00 | 32 | |
Temozolomide is a DNA alkylating agent that induces DNA methylation and damage, primarily at guanine bases. Pol ζ is recruited to repair the DNA lesions resulting from temozolomide treatment, preserving genomic integrity. | ||||||
Auramine O | 2465-27-2 | sc-206011 | 25 g | $52.00 | 1 | |
2-Aminopurine is a purine analog that can be incorporated into DNA during replication, leading to mispairing with thymine. Pol ζ participates in TLS to repair these mismatches and prevent mutations. | ||||||
Aphidicolin | 38966-21-1 | sc-201535 sc-201535A sc-201535B | 1 mg 5 mg 25 mg | $84.00 $306.00 $1104.00 | 30 | |
Aphidicolin is a reversible DNA polymerase inhibitor that slows down DNA replication, causing replication stress. Pol ζ is activated in response to replication stress to ensure proper DNA repair and maintain genomic stability. | ||||||
2,4-Dinitrophenol, wetted | 51-28-5 | sc-238345 | 250 mg | $59.00 | 2 | |
DNP is a chemical uncoupler that disrupts ATP production and induces replication stress. Pol ζ is involved in repairing DNA damage caused by replication stress to prevent genomic instability. | ||||||
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 50 g 100 g 250 g 1 kg 5 kg | $33.00 $67.00 $97.00 $192.00 $775.00 | 13 | |
Caffeine can modulate the DNA damage response pathway by inhibiting ATM and ATR kinase activity. This indirectly influences Pol ζ activation, as ATM and ATR kinases are involved in signaling DNA damage and repair responses. | ||||||