CSAGE, or Capture Selective Amplify and Gate, is a cutting-edge molecular biology technique designed to selectively target and analyze specific RNA transcripts from a complex mixture of RNA molecules. This method is particularly useful in transcriptomics, where it aids in the study of gene expression and regulation.The CSAGE technique involves several key steps. First, it uses molecular "capture" probes that are designed to specifically bind to the RNA transcripts of interest. These probes are typically short, complementary DNA or RNA sequences tailored to match the target RNA. Once bound, these probes allow for the selective isolation of the desired RNA from a complex sample, such as a cell or tissue extract.
Following capture, the targeted RNA undergoes an amplification process. This step is crucial because it increases the quantity of the RNA of interest, making it more detectable and analyzable. Amplification is typically achieved through techniques like PCR (Polymerase Chain Reaction), which can efficiently replicate specific RNA sequences.The final step, "gate," refers to the analysis and quantification of the amplified RNA. This can be done through various methods, such as sequencing or hybridization-based approaches. The data obtained provide insights into the abundance and variants of the targeted RNA transcripts, offering valuable information about gene expression patterns and regulation mechanisms. CSAGE is particularly advantageous in scenarios where precise and selective analysis of specific RNA molecules is required. It is used in research areas like cancer biology, developmental biology, and neuroscience, helping scientists understand complex gene expression dynamics in health and disease.
제품명 | CAS # | 카탈로그 번호 | 수량 | 가격 | 引用 | RATING |
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5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
DNA 메틸전달효소 억제제인 5-아자시티딘은 암세포의 침묵된 유전자를 재활성화하여 CSAGE 발현 또는 활성에 영향을 미칠 수 있습니다. | ||||||
Trichostatin A | 58880-19-6 | sc-3511 sc-3511A sc-3511B sc-3511C sc-3511D | 1 mg 5 mg 10 mg 25 mg 50 mg | $149.00 $470.00 $620.00 $1199.00 $2090.00 | 33 | |
히스톤 탈아세틸화 효소 억제제인 트리코스타틴 A는 염색질 구조를 변화시키며 CSAGE 발현에 간접적으로 영향을 미칠 수 있습니다. | ||||||
Suberoylanilide Hydroxamic Acid | 149647-78-9 | sc-220139 sc-220139A | 100 mg 500 mg | $130.00 $270.00 | 37 | |
트리코스타틴 A와 마찬가지로 보리노스타트는 히스톤 탈아세틸화 효소 억제제로, 유전자 발현을 변화시켜 CSAGE에 간접적으로 영향을 줄 수 있습니다. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $76.00 $216.00 | 101 | |
화학 요법 약물인 시스플라틴은 DNA 손상을 유도하여 암세포의 CSAGE 발현 또는 활성에 간접적으로 영향을 줄 수 있습니다. | ||||||
Doxorubicin | 23214-92-8 | sc-280681 sc-280681A | 1 mg 5 mg | $173.00 $418.00 | 43 | |
암 화학 요법에 사용되는 안트라사이클린계 항생제인 독소루비신은 DNA 손상과 세포 사멸을 유도하여 CSAGE 발현 세포에 영향을 미칠 수 있습니다. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $62.00 $155.00 $320.00 | 233 | |
mTOR 억제제인 라파마이신은 세포 성장과 증식을 조절하여 암세포의 CSAGE 기능에 잠재적으로 영향을 줄 수 있습니다. |