CSAGE, or Capture Selective Amplify and Gate, is a cutting-edge molecular biology technique designed to selectively target and analyze specific RNA transcripts from a complex mixture of RNA molecules. This method is particularly useful in transcriptomics, where it aids in the study of gene expression and regulation.The CSAGE technique involves several key steps. First, it uses molecular "capture" probes that are designed to specifically bind to the RNA transcripts of interest. These probes are typically short, complementary DNA or RNA sequences tailored to match the target RNA. Once bound, these probes allow for the selective isolation of the desired RNA from a complex sample, such as a cell or tissue extract.
Following capture, the targeted RNA undergoes an amplification process. This step is crucial because it increases the quantity of the RNA of interest, making it more detectable and analyzable. Amplification is typically achieved through techniques like PCR (Polymerase Chain Reaction), which can efficiently replicate specific RNA sequences.The final step, "gate," refers to the analysis and quantification of the amplified RNA. This can be done through various methods, such as sequencing or hybridization-based approaches. The data obtained provide insights into the abundance and variants of the targeted RNA transcripts, offering valuable information about gene expression patterns and regulation mechanisms. CSAGE is particularly advantageous in scenarios where precise and selective analysis of specific RNA molecules is required. It is used in research areas like cancer biology, developmental biology, and neuroscience, helping scientists understand complex gene expression dynamics in health and disease.
产品名称 | CAS # | 产品编号 | 数量 | 价格 | 应用 | 排名 |
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5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
作为一种 DNA 甲基转移酶抑制剂,5-氮杂胞苷可重新激活癌细胞中沉默的基因,从而可能影响 CSAGE 的表达或活性。 | ||||||
Trichostatin A | 58880-19-6 | sc-3511 sc-3511A sc-3511B sc-3511C sc-3511D | 1 mg 5 mg 10 mg 25 mg 50 mg | $149.00 $470.00 $620.00 $1199.00 $2090.00 | 33 | |
组蛋白去乙酰化酶抑制剂 Trichostatin A 可改变染色质结构,并可能间接影响 CSAGE 的表达。 | ||||||
Suberoylanilide Hydroxamic Acid | 149647-78-9 | sc-220139 sc-220139A | 100 mg 500 mg | $130.00 $270.00 | 37 | |
与 Trichostatin A 类似,Vorinostat 也是一种组蛋白去乙酰化酶抑制剂,可能会通过改变基因表达间接影响 CSAGE。 | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $76.00 $216.00 | 101 | |
化疗药物顺铂(Cisplatin)可诱导DNA损伤,从而间接影响癌细胞中CSAGE的表达或活性。 | ||||||
Doxorubicin | 23214-92-8 | sc-280681 sc-280681A | 1 mg 5 mg | $173.00 $418.00 | 43 | |
多柔比星是一种用于癌症化疗的蒽环类抗生素,它可能会通过诱导 DNA 损伤和细胞凋亡来影响表达 CSAGE 的细胞。 | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $62.00 $155.00 $320.00 | 233 | |
雷帕霉素是一种 mTOR 抑制剂,可调节细胞的生长和增殖,从而可能影响癌细胞中 CSAGE 的功能。 |