Date published: 2025-9-7
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YTHDF3 Antibody (F-2) - Western Blotting - Image 299344
YTHDF3 抗体 (F-2): sc-377119. AML-193 (A), U-87 (B) 和 SK-MEL-28 (C) 全细胞裂解液中 YTHDF3 表达的 Western 印迹分析.
YTHDF3 抗体 (F-2): sc-377119. AML-193 (A), U-87 (B) 和 SK-MEL-28 (C) 全细胞裂解液中 YTHDF3 表达的 Western 印迹分析.

YTHDF3 抗体 (F-2): sc-377119

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说明书
  • YTHDF3 抗体 F-2 是小鼠单克隆 IgG2a κ，YTHDF3抗体, 在23篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的YTHDF3的内部区域内的氨基酸85-119之间
  • YTHDF3 抗体 (F-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的YTHDF3; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗YTHDF3抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取YTHDF3 (F-2): sc-377119的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 YTHDF3 抗体 (F-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与YTHDF3 抗体 (F-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

YTHD 抗体

YTHDF3抗体（F-2）是一种IgG2a κ小鼠单克隆YTHDF3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源性YTHDF3蛋白。YTHDF3抗体（F-2）既有非偶联型抗YTHDF3抗体形式，也有多种偶联型抗YTHDF3抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。YTH结构域家族蛋白（YTHDF）包括YTHDF1、YTHDF2和TYHDF3。YTHDF1，也称为与癌症相关的皮肌炎假定自身抗原1（DACA-1），是一种含有一个YTH结构域（一种潜在的RNA结合结构域）的559个氨基酸的蛋白质。YTHDF2，也称为高糖调节蛋白8、CLL相关抗原KW-14或肾癌抗原NY-REN-2，是一种含有一个YTH结构域的579个氨基酸的蛋白质。YTHDF3是一种含有一个YTH结构域的585个氨基酸的蛋白质。编码YTHDF3的基因位于8号染色体上，该染色体编码约800个基因。在某些白血病和淋巴瘤中，发现了8号染色体部分片段的易位以及c-Myc基因的扩增，这通常与预后不良有关。8号染色体的部分片段与精神分裂症和双相情感障碍有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

YTHDF3 抗体 (F-2) 参考文献:

  1. 第 8 和第 10 染色体研讨会。  |  Wildenauer, DB. and Schwab, SG. 1999. Am J Med Genet. 88: 239-43. PMID: 10374738
  2. 在引入了正常 8 号染色体的维尔纳综合征细胞系中优先表达完整的 WRN 基因。  |  Kashino, G., et al. 2001. Biochem Biophys Res Commun. 289: 111-5. PMID: 11708785
  3. YTH：核蛋白中的一个新结构域。  |  Stoilov, P., et al. 2002. Trends Biochem Sci. 27: 495-7. PMID: 12368078
  4. 通过对 11 项躁狂症关联研究的综合分析，有力地证明了 6q 和 8q 染色体上的易感基因位点。  |  McQueen, MB., et al. 2005. Am J Hum Genet. 77: 582-95. PMID: 16175504
  5. 人类第 8 号染色体的 DNA 序列和分析。  |  Nusbaum, C., et al. 2006. Nature. 439: 331-5. PMID: 16421571
  6. 人类癌症中早衰 Werner 综合征基因的表观遗传失活。  |  Agrelo, R., et al. 2006. Proc Natl Acad Sci U S A. 103: 8822-7. PMID: 16723399
  7. 具有 Burkitt 样细胞的非霍奇金淋巴瘤与 c-Myc 扩增和预后不良缔合作用。  |  Mossafa, H., et al. 2006. Leuk Lymphoma. 47: 1885-93. PMID: 17065002
  8. 通过四面体分析法从部分孪生晶体中确定人胸腺细胞核蛋白 1 的 DUF55 域结构。  |  Yu, F., et al. 2009. Acta Crystallogr D Biol Crystallogr. 65: 212-9. PMID: 19237743

订购信息

产品名称产品编号规格价格数量收藏夹

YTHDF3 抗体 (F-2)

sc-377119
200 µg/ml
$316.00

YTHDF3 (F-2): m-IgG Fc BP-HRP 套装

sc-529978
200 µg Ab; 10 µg BP
$354.00

YTHDF3 (F-2): m-IgGκ BP-HRP 套装

sc-523196
200 µg Ab, 40 µg BP
$354.00

YTHDF3 (F-2): m-IgG2a BP-HRP 套装

sc-547412
200 µg Ab; 10 µg BP
$354.00

YTHDF3 抗体 (F-2) AC

sc-377119 AC
500 µg/ml, 25% agarose
$416.00

YTHDF3 抗体 (F-2) HRP

sc-377119 HRP
200 µg/ml
$316.00

YTHDF3 抗体 (F-2) FITC

sc-377119 FITC
200 µg/ml
$330.00

YTHDF3 抗体 (F-2) PE

sc-377119 PE
200 µg/ml
$343.00

YTHDF3 抗体 (F-2) Alexa Fluor® 488

sc-377119 AF488
200 µg/ml
$357.00

YTHDF3 抗体 (F-2) Alexa Fluor® 546

sc-377119 AF546
200 µg/ml
$357.00

YTHDF3 抗体 (F-2) Alexa Fluor® 594

sc-377119 AF594
200 µg/ml
$357.00

YTHDF3 抗体 (F-2) Alexa Fluor® 647

sc-377119 AF647
200 µg/ml
$357.00

YTHDF3 抗体 (F-2) Alexa Fluor® 680

sc-377119 AF680
200 µg/ml
$357.00

YTHDF3 抗体 (F-2) Alexa Fluor® 790

sc-377119 AF790
200 µg/ml
$357.00

YTHDF3 (F-2) 中和勝肽

sc-377119 P
100 µg/0.5 ml
$68.00

What is the recommended positive control for YTHDF3 Antibody (F-2): sc-377119?

Asked by: Trav11
Thank you for your question. For YTHDF3 Antibody (F-2): sc-377119, we have several recommended positive controls. The most common control for this antibody is HeLa Whole Cell Lysate (sc-2200). If you are interested in learning additional positive controls, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-01-16

What is the recommended secondary reagent for Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 5 out of 5 by from Works well to detect intracellular YTHDF3 by WBUsed this antibody for WB for a publication and it worked perfectly at a 1:2,000 dilution.
Date published: 2021-03-14
Rated 4 out of 5 by from Fairly great monoclonal antibodyUsed at 1:1000 for 12hr incubation at 4C. Performed on mouse cells and human cells. The YTHDF3 band has far more clarity on the human lysates.
Date published: 2017-08-29
Rated 5 out of 5 by from Produced beautiful immunofluorescence membraneProduced beautiful immunofluorescence membrane and cytoplasmic vesicles staining in methanol-fixed HeLa cells.
Date published: 2017-07-10
Rated 5 out of 5 by from GOOD FOR WBThis antibody does a good job in WB when it is used at 1:1000 with strong band and low background.
Date published: 2017-04-12
Rated 5 out of 5 by from Superb WB and IHC 1!Very nice western data; strong signal with low background; also outstanding IHC staining
Date published: 2017-01-24
Rated 5 out of 5 by from Good antibodyThis YTHDF3 antibody shows strong staining in western blotting.
Date published: 2017-01-14
Rated 5 out of 5 by from Beautiful immunoperoxidase nuclear and cytoplasmicBeautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Strong band in WB in CakiStrong band in WB in Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-02-15
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YTHDF3 抗体 (F-2) is rated 4.9 out of 5 by 9.
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