m-IgG_2a BP-HRP: sc-542731

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说明书

- 与辣根过氧化物酶(HRP)结合的小鼠IgG1₁结合蛋白 (m-IgG_2a BP)
- 规格为50 µg，体积0.5 ml
- 强烈推荐用来替代传统的抗小鼠IgG二抗，用于化学发光免疫印迹 (WB [ECL]) 和免疫组织化学 (IHC)
- 适合装订大部分，但不是全部，小鼠IgG_2a免疫球蛋白;不适合用于小鼠单克隆抗体IgG₁， IgG_2b， IgG₃， IgM, IgA或IgE抗体
- 不与人类或大鼠IgG抗体交叉反应
- 高特异性试剂，可产生强信号并能最大程度降低背景，几乎完全消除了传统方法生产的二抗所具有的批次间差异
- 一些单克隆抗体推荐使用小鼠IgG结合蛋白，但不是所有单克隆抗体都推荐使用。在我们的单克隆抗体产品页面上的产品描述将说明是否小鼠IgG结合蛋白是该产品的首选检测试剂。
- 也看看我们的新品m-IgG_2a BP-CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 和 CFL 790荧光染料偶联物。与传统的多克隆抗小鼠二抗荧光染料偶联物相比，这些具有实质性的改进。

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Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

DPPX (A-8): sc-365147. Western blot analysis of DPPX expression in DU 145 (A), IMR-32 (B), Neuro-2A (C), F9 (D) and AT3B-1

MCM7 (G-7): sc-46687. Western blot analysis of MCM7 expression in SHP-77 (A), COLO 320DM (B) and AMJ2-C8 (C) whole cell ly

TCP-1 β (F39 P7 F11): sc-47717. Western blot analysis of TCP-1 β expression in HEL 92.1.7 (A), Caki-1 (B), ZR-75-1 (C), F9

β3 Tubulin (AA10): sc-80016. Western blot analysis of β3 Tubulin expression in BJAB (A), A2058 (B), SK-N-SH (C), K-562 (D)

ERI-1 (B-10): sc-137089. Western blot analysis of ERI-1 expression in Jurkat (A), K-562 (B), JAR (C), NIH/3T3 (D), c4 (E)

HLA-DR (Bra30): sc-33717. Western blot analysis of HLA-DR expression in BJAB (A), Raji (B), U-698-M (C), GA-10 (D) and IB4

TFIIB (IIB8): sc-23875. Western blot analysis of TFIIB expression in K-562 (A), Hep G2 (B), Jurkat (C) and HeLa (D) nuclea

Tom20 (F-10): sc-17764. Western blot analysis of Tom20 expression in Raji (A), SW480 (B), Jurkat (C), Caki-1 (D), A549 (E)

VPS4 (E-8): sc-133122. Western blot analysis of VPS4 expression in ME-180 (A), NIH/3T3 (B) and Neuro-2A (C) whole cell lys

Laminin β-1 (D-9): sc-17763. Western blot analysis of Laminin β-1 expression in A-431 (A), JAR (B) and U-251-MG (C) whole

Bcl-x<sub>S/L</sub> (2H12): sc-23958. Western blot analysis of Bcl-x<sub>S/L</sub> expression in BJAB (A), NAMALWA (B), Ramos (C), HCT-116 (D) a

cyclin E (B-7): sc-48420. Western blot analysis of cyclin E expression in Jurkat nuclear extract (A) and A-673 (B), U-698-

CD45 (158-4D3): sc-52386. Western blot analysis of CD45 expression in U-698-M (A), NAMALWA (B) and GA-10 (C) whole cell ly

p53 (DO-1): sc-126. Western blot analysis of p53 expression in A549 (A), Daudi (B) and NTERA-2 cl.D1 (C) whole cell lysate

SR-A (E-5): sc-166184. Western blot analysis of SR-A expression in THP-1 (A) and CCRF-CEM (B) whole cell lysates and human

Pax-5 (A-11): sc-13146. Western blot analysis of Pax-5 expression in Ramos (A), Raji (B), NAMALWA (C) and IB4 (D) whole ce

HADHSC (A-5): sc-376525. Western blot analysis of HADHSC expression in Hep G2 (A), c4 (B), 3T3-L1 (C) and A-10 (D) whole c

RFC3 (G-10): sc-390293. Western blot analysis of RFC3 expression in MCF7 (A), GA-10 (B), Jurkat (C), RT-4 (D) and U-698-M

BRF2 (C-8): sc-390312. Western blot analysis of BRF2 expression in Jurkat (A), c4 (B), Neuro-2A (C) and F9 (D) whole cell

Peroxin 5 (B-3): sc-137103. Western blot analysis of Peroxin 5 expression in HeLa (A), SHP-77 (B), BT-20 (C) and SK-BR-3 (

X11α (A-12): sc-137022. Western blot analysis of X11α expression in mouse brain (A) and rat brain (B) tissue extracts. Det

NFATc2 (4G6-G5): sc-7296. Western blot analysis of NFATc2 expression in Ramos (A) and TK-1 (B) whole cell lysates. Detecti

Ig J chain (F-12): sc-133177. Western blot analysis of Ig J chain expression in non-transfected: sc-117752 (A) and human I

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介绍
说明书与实验方案

Mouse IgG_2a binding protein (m-IgG_2a BP) conjugated to horseradish peroxidase (HRP) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB) and immunohistochemical (IHC) signal enhancement. Mouse IgG_2a binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgG_2a binding protein (m-IgG_2a BP) is suitable for for binding to most, but not all mouse monoclonal IgG_2a antibodies; not suitable for use with mouse monoclonal IgG₁, IgG_2b, IgG₃, IgM, IgA or IgE antibodies. Not cross reactive with human or rat IgG antibodies.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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m-IgG_2a BP-HRP: sc-542731

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m-IgG2a BP-HRP: sc-542731

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m-IgG_2a BP-HRP: sc-542731