Date published: 2025-9-7
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V-ATPase A1 Antibody (E-8) - Western Blotting - Image 414583
V-ATPase A1抗体（E-8）：sc-374475。Western blot分析V-ATPase A1在未处理的HeLa细胞裂解液（A）和化学处理的HeLa细胞裂解液（B）中的表达。检测试剂：m-IgGκ BP-HRP：sc-516102。β-肌动蛋白 (C4)：sc-47778 用作内参。检测试剂：m-IgG Fc BP-HRP：sc-525409。
V-ATPase A1抗体（E-8）：sc-374475。Western blot分析V-ATPase A1在未处理的HeLa细胞裂解液（A）和化学处理的HeLa细胞裂解液（B）中的表达。检测试剂：m-IgGκ BP-HRP：sc-516102。β-肌动蛋白 (C4)：sc-47778 用作内参。检测试剂：m-IgG Fc BP-HRP：sc-525409。

V-ATPase A1 抗体 (E-8): sc-374475

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说明书
  • V-ATPase A1 抗体 E-8 是小鼠单克隆 IgG2b κ，V-ATPase A1抗体, 在10篇文献中引用，规格为200 µg/ml
  • 针对氨基酸71-210，在human起源的V-ATPase A1的细胞质域内进行映射
  • V-ATPase A1 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 V-ATPase A1; 也和以下物种反应，包括： 和 equine, bovine, porcine and canine
  • 抗V-ATPase A1抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取V-ATPase A1 (E-8): sc-374475的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是V-ATPase A1 Antibody (E-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与V-ATPase A1 Antibody (E-8) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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研究信息

関連項目

额外V-ATPase抗体，包括V-ATPase A1, V-ATPase α 1, V-ATPase B1, V-ATPase B2, V-ATPase C1, V-ATPase C2, V-ATPase D, V-ATPase D1, V-ATPase D2, V-ATPase G1, V-ATPase G2和V-ATPase H

V-ATPase A1抗体（E-8）是一种IgG2b κ小鼠单克隆V-ATPase A1抗体（也被命名为ATP6V0A1抗体、VPP1抗体、ATPase H+ Transporting V0 Subunit A1抗体、ATP6N1A抗体、ATP6N1抗体、Stv1抗体、Vph1抗体、A1抗体、ATPase H+ Transporting Lysosomal Non-Catalytic Accessory Protein 1 (110/116kD)抗体、V-ATPase 116 KDa Subunit A1抗体或V-ATPase 116 KDa抗体），它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的V-ATPase A1蛋白。V-ATPase A1抗体（E-8）既可以作为非共轭抗V-ATPase A1抗体形式，也可以作为多种共轭形式的抗V-ATPase A1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。液泡质子泵亚基是一种V-ATPase，具有两种不同的同工型。I型同工型含有一个18碱基对的插入片段，在脑中表达，而截短的II型同工型在肺、肾和脾等更广泛的部位表达。液泡质子泵亚基位于网格蛋白包被的囊泡中，也存在于破骨细胞中。它由两个基本结构域组成，一个亲水的氨基末端（含有超过30%的带电残基）和一个疏水的羧基末端（至少包含六个跨膜区）。质子泵的功能是将细胞质亚基的ATP水解与泵内膜成分的质子转运相耦合。破骨细胞特异性液泡质子ATP酶亚基的失活导致骨硬化突变小鼠破骨细胞顶端膜中缺乏该酶，从而阻止这些细胞的吸收功能，导致骨硬化表型。该亚基与液泡膜上的晚期内体标记物Rab7共定位，对于通过晚期内体的选择性膨胀形成液泡至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

V-ATPase A1 抗体 (E-8) 参考文献:

  1. 在骨硬化症（oc/oc）突变体中，编码人类破骨细胞特异性 116-kDa V-ATPase 亚基的小鼠同源基因出现缺失。  |  Scimeca, JC., et al. 2000. Bone. 26: 207-13. PMID: 10709991
  2. 水杨酰基酰胺A通过不同于巴法洛霉素A1的机制抑制V-ATP酶的V0区。  |  Xie, XS., et al. 2004. J Biol Chem. 279: 19755-63. PMID: 14998996
  3. 凝集素包被囊泡/突触囊泡质子泵 116 kDa 多肽的结构。  |  Perin, MS., et al. 1991. J Biol Chem. 266: 3877-81. PMID: 1704894
  4. 巴夫洛霉素A1通过抑制V-ATP酶依赖性酸化和Ca-P60A/SERCA依赖性自噬体-溶酶体融合来破坏自噬通量。  |  Mauvezin, C. and Neufeld, TP. 2015. Autophagy. 11: 1437-8. PMID: 26156798
  5. 视网膜色素上皮细胞中βA3/A1-晶状体对V-ATP酶的调节。  |  Valapala, M., et al. 2016. Adv Exp Med Biol. 854: 779-84. PMID: 26427489
  6. ABCA1（ATP 结合密码蛋白 A1）介导的胆固醇外流需要 V-ATPase（空泡 ATPase）活性。  |  Lorkowski, SW., et al. 2018. Arterioscler Thromb Vasc Biol. 38: 2615-2625. PMID: 30354238
  7. 质子泵V-ATP酶抑制剂巴法洛霉素A1影响溶酶体定位，并消除破骨细胞分泌性溶酶体的顺行性运输。  |  Matsumoto, N. and Nakanishi-Matsui, M. 2019. Biochem Biophys Res Commun. 510: 421-426. PMID: 30717974
  8. 巴佛洛霉素 A1 抑制 V-ATP 酶的分子基础  |  Wang, R., et al. 2021. Nat Commun. 12: 1782. PMID: 33741963
  9. 对V-ATP酶亚基a1和其他同工型中与疾病相关的突变进行结构和功能研究。  |  Indrawinata, K., et al. 2023. Front Mol Neurosci. 16: 1135015. PMID: 37465367
  10. 交替 mRNA 剪接产生液泡质子泵 116-kDa 多肽的组织特异性同工型。  |  Peng, SB., et al. 1994. J Biol Chem. 269: 17262-6. PMID: 8006034
  11. 幽门螺旋杆菌诱导的细胞内空泡上存在空泡 ATP 酶质子泵。  |  Papini, E., et al. 1996. J Med Microbiol. 45: 84-9. PMID: 8683556
  12. 液泡质子化反应 ATP 酶 116-kDa 亚基同工型的鉴定和重组。  |  Peng, SB., et al. 1999. J Biol Chem. 274: 2549-55. PMID: 9891027

订购信息

产品名称产品编号规格价格数量收藏夹

V-ATPase A1 抗体 (E-8)

sc-374475
200 µg/ml
$316.00

V-ATPase A1 (E-8): m-IgGκ BP-HRP 套装

sc-522752
200 µg Ab, 40 µg BP
$354.00

V-ATPase A1 抗体 (E-8) AC

sc-374475 AC
500 µg/ml, 25% agarose
$416.00

V-ATPase A1 抗体 (E-8) HRP

sc-374475 HRP
200 µg/ml
$316.00

V-ATPase A1 抗体 (E-8) FITC

sc-374475 FITC
200 µg/ml
$330.00

V-ATPase A1 抗体 (E-8) PE

sc-374475 PE
200 µg/ml
$343.00

V-ATPase A1 抗体 (E-8) Alexa Fluor® 488

sc-374475 AF488
200 µg/ml
$357.00

V-ATPase A1 抗体 (E-8) Alexa Fluor® 546

sc-374475 AF546
200 µg/ml
$357.00

V-ATPase A1 抗体 (E-8) Alexa Fluor® 594

sc-374475 AF594
200 µg/ml
$357.00

V-ATPase A1 抗体 (E-8) Alexa Fluor® 647

sc-374475 AF647
200 µg/ml
$357.00

V-ATPase A1 抗体 (E-8) Alexa Fluor® 680

sc-374475 AF680
200 µg/ml
$357.00

V-ATPase A1 抗体 (E-8) Alexa Fluor® 790

sc-374475 AF790
200 µg/ml
$357.00

The description for this antibody indicates it binds to the extracellular domain, but the papers it references points to the endogenous V0 subunit. Can this be clarified please?

Asked by: lucyh
Thank you for your question. This monoclonal antibody is raised against amino acids 71-210 mapping within a cytoplasmic domain of V-ATPase A1 of human origin. This information will be updated on our website.
Answered by: Technical Support
Date published: 2019-09-11

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, we do offer V-ATPase A1 (E-8): sc-374475 monoclonal antibody directly conjugated to HRP, and a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from worked great for murine samplesworked great for murine samples for western blott.
Date published: 2019-11-18
Rated 5 out of 5 by from My Review for V-ATPase A1 AntibodyAntibody works excellent for western blotting. Used at 1:500 in 5% BSA (overnight incubation). Detects a band of 150 kDa in cells lysed with non denaturing lysis buffer.
Date published: 2018-03-18
Rated 3 out of 5 by from Weak bandWe tried this antibody for WB in HEK293T cell lysates in 1:1000 dilution. We observed a weak band. Secondary antibody we used was anti-M IgG-680. The results may be improved by using M-IgGκ secondary antibodies.
Date published: 2017-11-14
Rated 5 out of 5 by from IHC detectionWe used this antibody by IHC to detect the expression of V-ATPase A1 in Human colon cancer tissue and normal tissue, V-ATPase was found high expression of V-ATPase A1 in colon cancer tissues, the positive result shows membrane and cytoplasm staining. The results obtained with this antibody are in line with our expectation of the experimental results. We suggest 1:200 dilution for IHC.
Date published: 2017-09-09
Rated 4 out of 5 by from Good antibodyvATPase-A1 antibody detected vATPase in N2A cell lysates by western. Nonspecific bands were also present. Primary 1:1000, secondary 1:10000. Thermo West femto reagent was used for detection.
Date published: 2017-08-11
Rated 5 out of 5 by from great featuresV-ATPase A1 Antibody (E-8) produced strong and specific signal by WB, IHC and IF in 293t cells.
Date published: 2017-04-12
Rated 5 out of 5 by from SpecificV-ATPase A1 Antibody (E-8) produced strong and specific signal by WB, IHC and IF
Date published: 2017-01-18
Rated 4 out of 5 by from Positive detection by WB using SKPositive detection by WB using SK-N-MC whole cell lysate. -SCBT QC
Date published: 2015-03-03
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V-ATPase A1 抗体 (E-8) is rated 4.6 out of 5 by 9.
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