TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4): sc-514992
- TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 7-28 at the N-terminus of TTF-1 of human origin
- recommended for detection of TTF-1 and Nkx-2.4 of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-514992 X, 200 µg/0.1 ml)
- See TTF-1/Thyroid Transcription Factor 1/NKX2-1 (8G7G3/1): sc-53136 for TTF-1/Thyroid Transcription Factor 1/NKX2-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4) for WB applications. These reagents are now offered in bundles with TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4) (see ordering information below).
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TTF-1 Antibody (E-4) is a mouse monoclonal IgG1 antibody that detects TTF-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TTF-1, also known as thyroid transcription factor-1, is a crucial member of the Nkx2 family of homeodomain-containing transcription factors, playing a vital role in regulating the transcriptional activity of thyroid-specific genes. TTF-1 is predominantly located in the epithelial cells of the lung, thyroid, and brain, where TTF-1 is essential for organogenesis and the maintenance of differentiated tissue phenotypes. In the lung, TTF-1 is instrumental in the transcription of surfactant proteins A, B, and C, which are critical for proper lung function and gas exchange. Additionally, in the thyroid, TTF-1 enhances the expression of key thyroid-specific markers, including thyroglobulin, thyroperoxidase, and thyrotropin receptors, thereby influencing thyroid hormone synthesis and regulation. TTF-1 also interacts with other proteins such as SRC-1 and CBP, which are important for transcriptional activity, highlighting TTF-1′s role in complex regulatory networks that govern cellular differentiation and function. Anti-TTF-1 antibody (E-4) is an invaluable tool for researchers studying these critical biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4) References:
- Clinical significance of TTF-1 protein expression and TTF-1 gene amplification in lung adenocarcinoma. | Barletta, JA., et al. 2009. J Cell Mol Med. 13: 1977-1986. PMID: 19040416
- TTF-1 expression in ovarian and uterine epithelial neoplasia and its potential significance, an immunohistochemical assessment with multiple monoclonal antibodies and different secondary detection systems. | Zhang, PJ., et al. 2009. Int J Gynecol Pathol. 28: 10-8. PMID: 19047914
- Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity. | Guazzi, S., et al. 1990. EMBO J. 9: 3631-9. PMID: 1976511
- Comparison of Three Different TTF-1 Clones in Resected Primary Lung Cancer and Epithelial Pulmonary Metastases. | Vidarsdottir, H., et al. 2018. Am J Clin Pathol. 150: 533-544. PMID: 30169783
- TTF-1 positive atypical carcinoid of the epiglottis mimicking medullary thyroid carcinoma. | Michalski, K., et al. 2019. Nuklearmedizin. 58: 279-281. PMID: 30974468
- The Incidence of Labelling of Non-Lung Adenocarcinomas With Antibodies Against TTF-1 and Diagnostic Implications. | Prabhakaran, S., et al. 2020. Appl Immunohistochem Mol Morphol. 28: 471-476. PMID: 31135446
- TTF-1 regulates lung epithelial morphogenesis. | Minoo, P., et al. 1995. Dev Biol. 172: 694-8. PMID: 8612983
- Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung. | Stahlman, MT., et al. 1996. J Histochem Cytochem. 44: 673-8. PMID: 8675988
- TTF-1 is an epithelial morphoregulatory transcriptional factor. | Minoo, P., et al. 1997. Chest. 111: 135S-137S. PMID: 9184563
- TTF-1 protein expression in pleural malignant mesotheliomas and adenocarcinomas of the lung. | Di Loreto, C., et al. 1998. Cancer Lett. 124: 73-8. PMID: 9500194
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4) | sc-514992 | 200 µg/ml | $322.00 | |||
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-541020 | 200 µg Ab; 10 µg BP | $361.00 | |||
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-542534 | 200 µg Ab; 20 µg BP | $361.00 | |||
TTF-1/Thyroid Transcription Factor 1/NKX2-1 (E-4) Neutralizing Peptide | sc-514992 P | 100 µg/0.5 ml | $69.00 | |||
TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (E-4) X | sc-514992 X | 200 µg/0.1 ml | $322.00 |