Date published: 2025-9-5
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TTF-1/Thyroid Transcription Factor 1/NKX2-1 Antibody (8G7G3/1) - Western Blotting - Image 370732
TTF-1 抗体 (8G7G3/1) HRP: sc-53136 HRP. 非转染 293T: sc-117752 (A), 人 TTF-1 转染 293T: sc-159187 (B), TT (C) 和 SHP-77 (D) 全细胞裂解液中 TTF-1 表达的直接 Western 印迹分析.
TTF-1 抗体 (8G7G3/1) HRP: sc-53136 HRP. 非转染 293T: sc-117752 (A), 人 TTF-1 转染 293T: sc-159187 (B), TT (C) 和 SHP-77 (D) 全细胞裂解液中 TTF-1 表达的直接 Western 印迹分析.

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1): sc-53136

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说明书
  • TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 8G7G3/1 是小鼠单克隆 IgG1 κ，TTF-1/Thyroid Transcription Factor 1/NKX2-1抗体, 在30篇文献中引用，规格为200 µg/ml
  • 针对全长重组TTF-1，rat起源
  • TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TTF-1
  • 抗TTF-1/Thyroid Transcription Factor 1/NKX2-1抗体（8G7G3/1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-53136 X, 200 µg/0.1 ml)
  • 关于如何获取TTF-1/Thyroid Transcription Factor 1/NKX2-1 (8G7G3/1): sc-53136的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 TTF 抗体 包括 TTF2

TTF-1抗体（8G7G3/1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的TTF-1蛋白。TTF-1抗体（8G7G3/1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。TTF-1，也称为甲状腺转录因子-1，是Nkx2家族中含同源结构域转录因子的重要成员，在调节甲状腺特异性基因的转录活性方面发挥着至关重要的作用。TTF-1主要位于肺、甲状腺和大脑的上皮细胞中，对于器官发生和维持分化表型至关重要。具体来说，TTF-1在肺形态发生中起着重要作用，因为它能够调节表面活性蛋白A、B和C的转录，而这些蛋白对于肺功能和气体交换至关重要。此外，在甲状腺中，TTF-1能够增强甲状腺特异性标记物的表达，如甲状腺球蛋白、甲状腺过氧化物酶和促甲状腺激素受体，从而影响甲状腺激素的合成和调节。TTF-1单克隆抗体（8G7G3/1）也被证明能够与包括SRC-1和CBP在内的其他蛋白质相互作用，这可能会进一步阐明TTF-1在转录调控和细胞信号通路中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) 参考文献:

  1. 甲状腺转录因子-1（TTF-1）在人类C细胞和甲状腺髓样癌中的表达。  |  Katoh, R., et al. 2000. Hum Pathol. 31: 386-93. PMID: 10746684
  2. 甲状腺球蛋白对甲状腺转录因子 1（TTF-1）基因表达的抑制是由控制组成型 TTF-1 表达的核因子 I 蛋白的 DNA 结合减少所介导的。  |  Nakazato, M., et al. 2000. Mol Cell Biol. 20: 8499-512. PMID: 11046146
  3. 人的 dio2 基因会受到甲状腺转录因子-1（TTF-1）的刺激，而大鼠的 dio2 基因则不会。  |  Gereben, B., et al. 2001. Mol Endocrinol. 15: 112-24. PMID: 11145743
  4. CBP/p300和SRC-1在甲状腺转录因子-1（TTF-1）对肺表面活性蛋白-A（SP-A）基因转录调控中的作用  |  Yi, M., et al. 2002. J Biol Chem. 277: 2997-3005. PMID: 11713256
  5. 甲状腺转录因子-1（TTF-1）在呼吸道上皮细胞中的表达增加会抑制肺泡化并导致肺部炎症。  |  Wert, SE., et al. 2002. Dev Biol. 242: 75-87. PMID: 11820807
  6. TTF-1在PACAP表达的视网膜神经节细胞中的表达  |  Son, YJ., et al. 2007. Mol Cells. 23: 215-9. PMID: 17464199
  7. TTF-1调节垂体前叶生长激素和催乳素的转录  |  Lee, NO., et al. 2007. Biochem Biophys Res Commun. 362: 193-199. PMID: 17706597
  8. 两例胃肠道腺癌的 TTF-1 阳性。  |  Pegolo, E., et al. 2014. Appl Immunohistochem Mol Morphol. 22: e27-31. PMID: 25247229
  9. 甲状腺转录因子-1（TTF-1）磷酸化位点的图谱和功能作用。  |  Zannini, M., et al. 1996. J Biol Chem. 271: 2249-54. PMID: 8567686
  10. γ干扰素通过减少甲状腺转录因子-1（TTF-1）与其识别位点的结合来抑制促甲状腺激素受体启动子的活性。  |  Ohe, K., et al. 1996. Mol Endocrinol. 10: 826-36. PMID: 8813723
  11. 甲状腺转录因子-1（TTF-1）基因从一个新定义的起始位点开始转录：TTF-1在甲状腺中的正调控。  |  Nakazato, M., et al. 1997. Biochem Biophys Res Commun. 238: 748-52. PMID: 9325161
  12. 甲状腺特异性增强子结合蛋白（T/EBP）/甲状腺特异性转录因子-1（TTF-1）基因编码的多个转录本：自调节的证据。  |  Oguchi, H. and Kimura, S. 1998. Endocrinology. 139: 1999-2006. PMID: 9528987

订购信息

产品名称产品编号规格价格数量收藏夹

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1)

sc-53136
200 µg/ml
$316.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 (8G7G3/1): m-IgG Fc BP-HRP 套装

sc-526772
200 µg Ab; 10 µg BP
$354.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 (8G7G3/1): m-IgG1 BP-HRP 套装

sc-532145
200 µg Ab; 20 µg BP
$354.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) X

sc-53136 X
200 µg/0.1 ml
$316.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) AC

sc-53136 AC
500 µg/ml, 25% agarose
$416.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) HRP

sc-53136 HRP
200 µg/ml
$316.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) FITC

sc-53136 FITC
200 µg/ml
$330.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) PE

sc-53136 PE
200 µg/ml
$343.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) Alexa Fluor® 488

sc-53136 AF488
200 µg/ml
$357.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) Alexa Fluor® 546

sc-53136 AF546
200 µg/ml
$357.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) Alexa Fluor® 594

sc-53136 AF594
200 µg/ml
$357.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) Alexa Fluor® 647

sc-53136 AF647
200 µg/ml
$357.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) Alexa Fluor® 680

sc-53136 AF680
200 µg/ml
$357.00

TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) Alexa Fluor® 790

sc-53136 AF790
200 µg/ml
$357.00

Is this antibody suitable for immunoprecipitation?

Asked by: ama027
Thank you for your question. sc-53136 TTF-1 (8G7G3/1) is recommended for WB, IP, PAR, and ELISA. https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: BlakeJ
Date published: 2024-05-30

Is this antibody reactive with porcine lung tissue?

Asked by: Jacky24
Thank you for your question. We have not tested this antibody in porcine lung tissue, nor are we aware of any product citations in porcine samples. Please contact our Technical Service Department and we can perform sequence analysis of the antibody's antigen to predict potential cross reactivity with the porcine protein.
Answered by: Technical Support
Date published: 2019-03-05

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TTF-1 (8G7G3/1): sc-53136?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13
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Rated 1 out of 5 by from Undetected ProteinNot possible to reveal the protein in samples at working dilution: Embryonic mouse coronal sections – E16.5 – working dilution : 1:50 Antigen retrieval was performed with citrate buffer.
Date published: 2023-07-28
Rated 5 out of 5 by from Good results on feline and canine thyroidTried with 1:50 and HIER (pH 9.0). Specific immunolabeling.
Date published: 2021-02-12
Rated 5 out of 5 by from Great IHC !!Strong signal with low background in Immunohistochemistry (IHC)
Date published: 2020-02-19
Rated 5 out of 5 by from 挺好用的用的试用装，1:500的稀释比做WB，挺好显的，条带也单一，其他类型的实验倒是没有做过，推荐大家使用
Date published: 2019-04-18
Rated 5 out of 5 by from Great Ab for EMSAsThis is a great Ab for gel shifts (see arrow in the picture). Order the concentrated version.
Date published: 2017-08-18
Rated 5 out of 5 by from Excellent antibody!This antibody works very well in lung cancer cells.I bought this a month ago and am so happy that I did pefect results use this antibody for WB.The dilution that I used was 1:500 and it's specific.
Date published: 2017-08-15
Rated 4 out of 5 by from 试用装抗体评论前段时间申请的TTF-1试用装跑WB显影，有条带但是好像位置不对，结合RT结果，有可能是样品里面本身不含有该蛋白
Date published: 2017-08-11
Rated 5 out of 5 by from Rat Medial Ganglionic Eminence Cell SmearThis antibody works well on medial ganglionic eminence (MGE) cells from rat. I tried a TTF-1 (NKX2.1) antibody from another vendor and it did not work. I am happy I was able to find an antibody that worked for these cells.
Date published: 2017-08-08
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TTF-1/Thyroid Transcription Factor 1/NKX2-1 抗体 (8G7G3/1) is rated 4.4 out of 5 by 14.
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