Date published: 2025-9-8
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Topo I Antibody (C-21) - Western Blotting - Image 356625
Topo I 抗体 (C-21): sc-32736. 在 Ramos (A), Jurkat (B), SK-N-SH (C), HEL 92. 1. 7 (D) 和 Daudi (E) 全细胞裂解液中 Topo I 表达的 Western 印迹分析.
Topo I 抗体 (C-21): sc-32736. 在 Ramos (A), Jurkat (B), SK-N-SH (C), HEL 92. 1. 7 (D) 和 Daudi (E) 全细胞裂解液中 Topo I 表达的 Western 印迹分析.

Topo I 抗体 (C-21): sc-32736

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说明书
  • Topo I 抗体 C-21 是小鼠单克隆 IgM κ，Topo I抗体, 在31篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组Topo I
  • 推荐用于 mouse, rat 和 human 来源的Topo I WB, IP, IF 和 IHC(P)检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取Topo I (C-21): sc-32736的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Topo I 抗体 (C-21) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Topo I 抗体 (C-21) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Topo 抗体 包括 Topo I, Topo II, Topo II α, Topo II β和Topo III β-1

Topo I 抗体（C-21）是一种小鼠单克隆IgM抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的Topo I蛋白。抗拓扑异构酶 I 抗体（C-21）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。拓扑异构酶I（Topo I）蛋白，也称为DNA拓扑异构酶I，在细胞复制、转录和重组等过程中维持DNA结构完整性方面发挥着至关重要的作用。拓扑异构酶I通过在DNA螺旋的一条链上引入瞬时断裂，减轻复制叉前产生的扭转应力，防止DNA超螺旋，确保DNA的正常功能。这种酶活性对于细胞健康至关重要，因为拓扑异构酶I的失调会导致基因组不稳定，并可能引发多种癌症，因此拓扑异构酶I成为抗癌疗法的重要靶标。拓扑异构酶I与多种其他蛋白质相互作用，包括转录因子和修复蛋白，这进一步凸显了拓扑异构酶I在细胞过程中的重要性，并凸显了拓扑异构酶I作为治疗靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Topo I 抗体 (C-21) 参考文献:

  1. 拓扑异构酶 II alpha 和拓扑异构酶 II beta 基因：特性分析及分别与人类第 17 和 3 号染色体的映射关系。  |  Tan, KB., et al. 1992. Cancer Res. 52: 231-4. PMID: 1309226
  2. 人类 I 型 DNA 拓扑异构酶基因的结构。  |  Kunze, N., et al. 1991. J Biol Chem. 266: 9610-6. PMID: 1851751
  3. 从 HeLa 细胞中分离并鉴定编码新型 DNA 拓扑异构酶 II 同源物的人类 cDNA 克隆。  |  Austin, CA. and Fisher, LM. 1990. FEBS Lett. 266: 115-7. PMID: 2163884
  4. 编码两种人类 DNA 拓扑异构酶 II 同工酶的 cDNA 克隆的特征和免疫学鉴定。  |  Chung, TD., et al. 1989. Proc Natl Acad Sci U S A. 86: 9431-5. PMID: 2556712
  5. 人 DNA 拓扑异构酶 I 的 cDNA 克隆：67.7-kDa 羧基端片段的催化活性。  |  D'Arpa, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 2543-7. PMID: 2833744
  6. 槐树种子中两种新型胞嘧啶类生物碱二聚体的发现、Topo I 抑制活性和机制评估  |  Meng, C., et al. 2022. Bioorg Med Chem. 61: 116723. PMID: 35396127
  7. 含铜离子的盐酸吡哆醛硫代氨基甲酸盐通过 Topo-I 和 Topo-IIɑ 抑制细胞分裂。  |  Qi, J., et al. 2022. J Inorg Biochem. 232: 111816. PMID: 35405490
  8. 发现新型苯并呋喃并[3,2-b]喹啉衍生物作为 CDK2/Topo I 双重抑制剂。  |  Huang, Y., et al. 2022. Bioorg Chem. 126: 105870. PMID: 35636125
  9. (+)-Pancratistatin 的全合成及其强效拓扑 I 抑制活性研究。  |  Ding, F., et al. 2022. Org Lett. 24: 9458-9462. PMID: 36522148
  10. 设计和合成新的螺吲哚候选化合物及其硒纳米颗粒，作为潜在的 Topo I/II 双重抑制剂、DNA 中间体和细胞凋亡诱导剂。  |  El-Kalyoubi, S., et al. 2023. J Enzyme Inhib Med Chem. 38: 2242714. PMID: 37592917
  11. DNA 拓扑异构酶的机制。  |  Roca, J. 1995. Trends Biochem Sci. 20: 156-60. PMID: 7770916
  12. 人类拓扑异构酶 I 的机制模型。  |  Stewart, L., et al. 1998. Science. 279: 1534-41. PMID: 9488652

订购信息

产品名称产品编号规格价格数量收藏夹

Topo I 抗体 (C-21)

sc-32736
200 µg/ml
$316.00

Topo I (C-21): m-IgGκ BP-HRP 套装

sc-520854
200 µg Ab, 40 µg BP
$354.00

Topo I 抗体 (C-21) AC

sc-32736 AC
500 µg/ml, 25% agarose
$416.00

Topo I 抗体 (C-21) Alexa Fluor® 488

sc-32736 AF488
200 µg/ml
$357.00

Topo I 抗体 (C-21) Alexa Fluor® 647

sc-32736 AF647
200 µg/ml
$357.00

Topo I 抗体 (C-21) FITC

sc-32736 FITC
200 µg/ml
$330.00

Topo I 抗体 (C-21) HRP

sc-32736 HRP
200 µg/ml
$316.00

Topo I 抗体 (C-21) PE

sc-32736 PE
200 µg/ml
$343.00

What is the position of the epitope of Topoisomerase I against which this antibody is raised??

Asked by: Pooja311
Thank you for your question. This Topoisomerase antibody was raised against recombinant Topo I of human origin. The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-10-18

Why there are more than two forms? Are they two isoforms of TOPO1 beta ?

Asked by: sheraz
Thank you for your question. The expected molecular weight for Topo 1 is ~91-100kDa. This is dependent on glycosylation as it is a highly glycosylated protein. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2019-08-12

Does Topo I (c-21) sc-32736 work well in RIP/CLIP experiments, using standard protein A/G beads?

Asked by: fra3
Thank you for your question. We have not tested this mouse monoclonal IgM, Topo I Antibody (C-21): sc-32736, for RIP or CLIP experiments, and so those applications are not recommended at this time. This antibody is recommended for WB, IP, IF and IHC(P).
Answered by: Technical Support
Date published: 2017-04-24

Can Topo I (C-21): sc-32736 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Topo I (C-21): sc-32736 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 14
Date published: 2017-02-07
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Rated 1 out of 5 by from The Ab does not work for ICC at allThe antibody were supposed to detect TOP1 by ICC and the example staining was provided in the description< which shows primarily nuclear staining and robust nicleoli staining. We performed staining for TOP1 both in PFA fixed and Triton X-100-permeabilized cells or MeOH-fixed cells usin the range of the dilutions - from 1:50 to 1:500 - no success at all
Date published: 2020-02-24
Rated 3 out of 5 by from Staining is strong, location is not precisewe used this antibody for our IHC stain on tumour sample, the TOPOⅠ protein located in cell nucleus. In our tissue slice, the nucleus is positive, colour is strong, but at the same time, cytoplasm is positive too.
Date published: 2017-10-19
Rated 5 out of 5 by from a good antibody for both WB and IFThis antibody generates a signal at the predicted position on the western blot (1:500 dilution, 40ug HeLa extract). It also stains the nucleus with a stronger signal in the nucleoli.
Date published: 2017-09-01
Rated 5 out of 5 by from The preferred choice for IHC(P)This mouse monoclonal IgM antibody, clone (C-21) is the preferred choice for detection of human Topo I for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections.
Date published: 2017-01-24
Rated 4 out of 5 by from Publishable data in IHC(P) using fish liverPublishable data in IHC(P) using fish liver tissue -SCBT Publication Review
Date published: 2015-05-08
Rated 3 out of 5 by from Robust band seen in WB using KB cellsRobust band seen in WB using KB cells. -SCBT QC
Date published: 2015-04-17
Rated 3 out of 5 by from Positive result in WB using KPositive result in WB using K-562 and HeLa nuclear extracts. -SCBT QC
Date published: 2015-03-06
Rated 4 out of 5 by from Publishable data in WB using human AP3Publishable data in WB using human AP3, AP8-CDC cells. -SCBT Publication Review
Date published: 2015-01-23
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Topo I 抗体 (C-21) is rated 3.7 out of 5 by 9.
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