Date published: 2025-9-7
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SPARC Antibody (AON-1) - Western Blotting - Image 398279
SPARC 抗体 (AON-1): sc-33645. A-375 (A), BJ (B) 和 U-251-MG (C) 全细胞裂解液中 SPARC 表达的 Western 印迹分析. 所用检测试剂: m-IgG Fc BP-HRP: sc-525409.
SPARC 抗体 (AON-1): sc-33645. A-375 (A), BJ (B) 和 U-251-MG (C) 全细胞裂解液中 SPARC 表达的 Western 印迹分析. 所用检测试剂: m-IgG Fc BP-HRP: sc-525409.

SPARC 抗体 (AON-1): sc-33645

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说明书
  • SPARC抗体(AON-1)是小鼠单克隆IgG3 κ, 在8篇文献中引用，规格为200 µg/ml
  • 针对bovine骨骼SPARC
  • 推荐用于 mouse, rat 和 human 来源的SPARC WB, IP 和 IF检测; 也和以下物种反应，包括： bovine
  • 看 SPARC (D-2): sc-398419，可查看有标记的 SPARC 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取SPARC (AON-1): sc-33645的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是SPARC Antibody (AON-1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与SPARC Antibody (AON-1) 搭配使用（请参阅下面的订购信息）。

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研究信息

関連項目

附加SPARC抗体，包括SPARCL1

SPARC抗体（AON-1）是一种IgG3 κ小鼠单克隆SPARC抗体，可通过WB、IP和IF检测来自小鼠、大鼠和人类的SPARC。SPARC抗体（AON-1）可以是非偶联的抗SPARC抗体形式使用。SPARC（即富含硫氨酸的酸性分泌蛋白）是一种富含甘氨酸的磷酸化酸性糖蛋白，由内皮细胞分泌，在小鼠胚胎的腹壁内胚层中大量存在，并在人类胎盘中也有存在。它与骨连结蛋白完全相同，骨连结蛋白是一种对骨钙化非常重要的蛋白，在物种之间高度保守。SPARC可以在受到某些类型的损伤时被内皮细胞选择性地表达，在体外诱导内皮细胞黏附变圆。它通过F-Actin依赖的细胞形态变化来调节内皮屏障功能，与细胞间缝隙的出现同时发生，为大分子的跨膜转运提供了一条细胞间通道。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

SPARC 抗体 (AON-1)

sc-33645
200 µg/ml
$316.00

SPARC (AON-1): m-IgG Fc BP-HRP 套装

sc-525540
200 µg Ab; 10 µg BP
$354.00

We are wondering the species of which we can use the secondary antibody. We are confuse with the bovine portion of it

Asked by: Harmin Vallese
Thank you for your question. sc-33645 SPARC (AON-1) is reactive with mouse, rat, human, cow, monkey, pig, dog and rabbit . We recommend using an anti-mouse IgG3 secondary . https://www.scbt.com/browse/Anti-Mouse-IgG-Binding-Protein-Secondaries/_/N-130b1ry
Answered by: BlakeJ
Date published: 2023-01-11

Are there any issues with using sc-33645: SPARC (AON-1) mouse monoclonal primary antibody on mouse samples?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-33645 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Western blot using Anti-SPARC Antibodysc-33645the antibody work great in primary lung fibroblast, primary lung epithelial cells and cell line
Date published: 2021-07-02
Rated 5 out of 5 by from Excellent antibody for WBThis antibody for SPARC works very good for WB in adipose tissue.
Date published: 2017-10-20
Rated 5 out of 5 by from Positve resultsPositive results obtained with SPARC Antibody (AON-1) in various samples
Date published: 2017-02-01
Rated 4 out of 5 by from Publishable data in WB using human transfectedPublishable data in WB using human transfected HT1080 cells -SCBT Publication Review
Date published: 2015-06-13
Rated 4 out of 5 by from Gives a very good signal by WB (dilGives a very good signal by WB (dil. 1/20) with low background. Peer Review
Date published: 2015-06-12
Rated 4 out of 5 by from Bands seen in WB using human recombinantBands seen in WB using human recombinant SPARC and SPARC expression in A549 whole cell lysate. -SCBT QC
Date published: 2015-06-09
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-22
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SPARC 抗体 (AON-1) is rated 4.4 out of 5 by 7.
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