Date published: 2025-9-7

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SPARC 抗体 (D-2): sc-398419

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说明书
  • SPARC 抗体 D-2 是小鼠单克隆 IgG2b κ,SPARC抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫human物种的SPARC的氨基酸1-90
  • SPARC 抗体 (D-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 SPARC
  • 抗SPARC抗体(D-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SPARC (D-2): sc-398419免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 SPARC 抗体 (D-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SPARC 抗体 (D-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SPARC抗体(D-2)是一种IgG2b κ小鼠单克隆SPARC抗体(也称为骨连蛋白抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源的SPARC蛋白。SPARC抗体(D-2)既有非偶联形式的抗SPARC抗体,也有多种偶联形式的抗SPARC抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SPARC(即富含硫氨酸的酸性分泌蛋白)是一种富含甘氨酸的磷酸化酸性糖蛋白,由内皮细胞分泌,在小鼠胚胎的腹壁内胚层中大量存在,并在人类胎盘中也有存在。它与骨连结蛋白完全相同,骨连结蛋白是一种对骨钙化非常重要的蛋白,在物种之间高度保守。SPARC可以在受到某些类型的损伤时被内皮细胞选择性地表达,在体外诱导内皮细胞黏附变圆。它通过F-Actin依赖的细胞形态变化来调节内皮屏障功能,与细胞间缝隙的出现同时发生,为大分子的跨膜转运提供了一条细胞间通道。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SPARC 抗体 (D-2)

sc-398419
200 µg/ml
$316.00

SPARC (D-2): m-IgGκ BP-HRP 套装

sc-524330
200 µg Ab, 40 µg BP
$354.00

SPARC (D-2): m-IgG2b BP-HRP 套装

sc-549160
200 µg Ab; 10 µg BP
$354.00

SPARC 抗体 (D-2) AC

sc-398419 AC
500 µg/ml, 25% agarose
$416.00

SPARC 抗体 (D-2) HRP

sc-398419 HRP
200 µg/ml
$316.00

SPARC 抗体 (D-2) FITC

sc-398419 FITC
200 µg/ml
$330.00

SPARC 抗体 (D-2) PE

sc-398419 PE
200 µg/ml
$343.00

SPARC 抗体 (D-2) Alexa Fluor® 488

sc-398419 AF488
200 µg/ml
$357.00

SPARC 抗体 (D-2) Alexa Fluor® 546

sc-398419 AF546
200 µg/ml
$357.00

SPARC 抗体 (D-2) Alexa Fluor® 594

sc-398419 AF594
200 µg/ml
$357.00

SPARC 抗体 (D-2) Alexa Fluor® 647

sc-398419 AF647
200 µg/ml
$357.00

SPARC 抗体 (D-2) Alexa Fluor® 680

sc-398419 AF680
200 µg/ml
$357.00

SPARC 抗体 (D-2) Alexa Fluor® 790

sc-398419 AF790
200 µg/ml
$357.00

FREE 10 µg sample of SPARC (D-2): sc-398419. Can I have this antibody for free trial?

Asked by: morncafe
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-22

Can SPARC (D-2): sc-398419 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, SPARC (D-2): sc-398419 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Working perfectThis antibody stained the target perfectly for the immunofluorescent staining of cells.
Date published: 2017-12-13
Rated 5 out of 5 by from excellenttested on rat tissue samples in western blot and worked perfectly.
Date published: 2017-12-13
Rated 5 out of 5 by from Successful application of this antibody in IFSuccessful application of this antibody in IF, using human tissues.
Date published: 2017-09-17
Rated 5 out of 5 by from Very easy to useThe first time I used this antibody it gave excellent WB signals. No problem, will continue to use.
Date published: 2017-05-22
Rated 5 out of 5 by from Positive result in western blot analysisPositive result in western blot analysis of sparc expression in human cartilage tissue extract. -ZGL
Date published: 2017-04-17
Rated 5 out of 5 by from Powerful antibodiesPowerful antibodies work by the bank with pancreatic cancer cell line SPARC WB and ELISA detection
Date published: 2017-02-11
Rated 5 out of 5 by from specific bandsThe specific bands were detected by Western blot and 1:500 in human tissues.
Date published: 2017-02-09
Rated 5 out of 5 by from Excellent choice for SPARC detection!This is a strong and reliable antibody for both IF and WB. Can't go wrong: nice and clean!
Date published: 2017-01-18
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SPARC 抗体 (D-2) is rated 4.8 out of 5 by 12.
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