RLF Antibody (E-4): sc-393670

RLF Antibody (E-4) is a mouse monoclonal IgG2a antibody that detects RLF in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RLF, or Ras-associated protein Rab2L/RalGDS-like factor, plays a crucial role as a guanine nucleotide exchange factor (GEF) for the small GTPase Ral, acting as a key intermediary in the Ras signaling pathway. RLF is primarily located in the cytoplasm and is essential for mediating Ras-dependent signaling, which is vital for cellular processes such as proliferation and differentiation. RLF interaction with the GTP-bound form of Ras proteins through its C-terminal Ras-binding domain facilitates the activation of Ral, leading to the phosphorylation of c-Jun at serines 63 and 73. This phosphorylation enhances c-Jun′s transcriptional activity, thereby influencing gene expression related to cell growth and transformation. RLF′s ability to induce transcriptional activation and promote cell division, particularly in neuroretina cells, underscores its importance in developmental processes and potential implications in oncogenic signaling pathways. Anti-RLF antibody (E-4) serves as an invaluable tool for researchers studying the intricate mechanisms of Ras signaling and its impact on cellular behavior.

The preferred RLF antibody is RLF Antibody (D-7): sc-365373.