Date published: 2025-9-6

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podoplanin 抗体 (8.1.1): sc-53533

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说明书
  • podoplanin抗体(8.1.1)是一种叙利亚仓鼠单克隆 IgG podoplanin抗体,在17篇出版物中被引用,在200 µg/ml处提供
  • 针对mouse的胸腺上皮细胞系
  • podoplanin 抗体 (8.1.1) 推荐用于WB, IP 和 IF,检测mouse 来源的 podoplanin; 不会与 human podoplanin产生交联反应
  • 抗podoplanin抗体(8.1.1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取podoplanin (8.1.1): sc-53533免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成podoplanin 抗体 (8.1.1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Podoplanin 抗体(8.1.1)是一种叙利亚仓鼠单克隆 IgG 抗体,采用 Western 印迹 (WB)、免疫沉淀 (IP) 和免疫荧光 (IF) 技术特异性检测小鼠样本中的 Podoplanin(又称糖蛋白 38 (gp38)、T1-α、Aggrus 或 PA2.26 抗原)。抗 Podoplanin 抗体(8.1.1)是针对小鼠胸腺上皮细胞系提出的。Podoplanin 是一种 I 型跨膜糖蛋白,主要定位于外周淋巴组织的基质细胞和胸腺上皮细胞,Podoplanin 在维持肾脏中荚膜细胞的结构完整性和独特形态方面起着至关重要的作用。Podoplanin 在细胞膜中的精确定位对于调节淋巴内皮细胞功能和促进关键的细胞-细胞相互作用至关重要。通过 Podoplanin 单克隆抗体 (8.1.1) 可以对小鼠模型中的淋巴管生成、免疫细胞迁移和荚膜细胞生物学进行详细研究。此外,抗 Podoplanin 抗体 (8.1.1) 既有非共轭形式,也有多种共轭形式,包括辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 变体,为各种实验应用提供了多样性。Podoplanin (8.1.1) 抗体是研究人员探索 Podoplanin 在免疫和肾脏系统的细胞结构和功能中的多方面作用的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

podoplanin 抗体 (8.1.1) 参考文献:

  1. 用抗波多野苷免疫染色法评估人类乳腺癌的淋巴微血管密度和淋巴管侵袭。  |  Schoppmann, SF., et al. 2001. Anticancer Res. 21: 2351-5. PMID: 11724292
  2. 肺 I 型细胞分化基因 T1alpha 是出生时正常肺细胞增殖和肺泡形成所必需的。  |  Ramirez, MI., et al. 2003. Dev Biol. 256: 61-72. PMID: 12654292
  3. 小鼠胸腺 I 型上皮细胞优先表达的抗原决定因子的特征。  |  Farr, A., et al. 1992. J Histochem Cytochem. 40: 651-64. PMID: 1374092
  4. 外周淋巴组织 T 依赖区基质细胞表达的新型糖蛋白的特征和克隆。  |  Farr, AG., et al. 1992. J Exp Med. 176: 1477-82. PMID: 1402691
  5. 端粒酶转染的人淋巴管内皮细胞的生成和特征描述。  |  Nisato, RE., et al. 2004. Am J Pathol. 165: 11-24. PMID: 15215158
  6. IL-3 可诱导人内皮细胞表达淋巴标志物 Prox-1 和 podoplanin。  |  Gröger, M., et al. 2004. J Immunol. 173: 7161-9. PMID: 15585837
  7. 淋巴结成纤维网状细胞表达的一种跨膜趋化因子--CXC趋化因子配体16--有可能调节T细胞的迁移和粘附。  |  Hara, T., et al. 2006. Int Immunol. 18: 301-11. PMID: 16410312
  8. E11/gp38 在骨细胞中的选择性表达:机械应变的调节和在树突伸长中的作用。  |  Zhang, K., et al. 2006. Mol Cell Biol. 26: 4539-52. PMID: 16738320

订购信息

产品名称产品编号规格价格数量收藏夹

podoplanin 抗体 (8.1.1)

sc-53533
200 µg/ml
$316.00

podoplanin 抗体 (8.1.1) AC

sc-53533 AC
500 µg/ml, 25% agarose
$416.00

podoplanin 抗体 (8.1.1) HRP

sc-53533 HRP
200 µg/ml
$316.00

podoplanin 抗体 (8.1.1) FITC

sc-53533 FITC
200 µg/ml
$330.00

podoplanin 抗体 (8.1.1) PE

sc-53533 PE
200 µg/ml
$343.00

podoplanin 抗体 (8.1.1) Alexa Fluor® 488

sc-53533 AF488
200 µg/ml
$357.00

podoplanin 抗体 (8.1.1) Alexa Fluor® 546

sc-53533 AF546
200 µg/ml
$357.00

podoplanin 抗体 (8.1.1) Alexa Fluor® 594

sc-53533 AF594
200 µg/ml
$357.00

podoplanin 抗体 (8.1.1) Alexa Fluor® 647

sc-53533 AF647
200 µg/ml
$357.00

podoplanin 抗体 (8.1.1) Alexa Fluor® 680

sc-53533 AF680
200 µg/ml
$357.00

podoplanin 抗体 (8.1.1) Alexa Fluor® 790

sc-53533 AF790
200 µg/ml
$357.00

Do you have suitable secondary antibodies for this sc-53533 (IF)?

Asked by: tony1
Thank you for your question. podoplanin Antibody (8.1.1) is a Syrian hamster monoclonal IgG. Unfortunately, at this time we do not currently offer a secondary antibody that is anti-Syrian hamster IgG. As an alternative, this primary antibody is available directly conjugated to a variety of fluorochromes that would be suitable for IF including Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594, Alexa Fluor® 647, phycoerythrin or FITC.
Answered by: Technical Support
Date published: 2021-09-20

Can I use this antibody on human samples?

Asked by: TinTin
No, unfortunately this antibody is only validated and warrantied for use on mouse samples at this time. We have several other antibodies that are human reactive, you can find them here: https://www.scbt.com/scbt/browse/antibodies-Primary-Antibodies-lymphocyte-signaling-antibodies-podoplanin-Antibodies/_/N-1q7bhky
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from Works on IF on mouse tissueWorks well on mouse paraffin embedded tissue for IF.
Date published: 2017-09-01
Rated 5 out of 5 by from Good antibody for IFWe used this antibody for detecting PDPN in IF. It worked well!
Date published: 2017-08-03
Rated 5 out of 5 by from very goodit is very good for this sample,dilution:1:100,for IF,ICH
Date published: 2017-08-03
Rated 5 out of 5 by from Nice resultsUsed podoplanin Antibody (8.1.1) under reducing and non-reducing conditions by WB and the results were specific and strong.
Date published: 2017-01-13
Rated 5 out of 5 by from 4 g/ml used for IHC with paraffin embedded4 g/ml used for IHC with paraffin embedded murine lung and skin samples; also used for western blotting. -SCBT Publication Review
Date published: 2015-05-02
Rated 5 out of 5 by from Robust band observed in western blot analysisRobust band observed in western blot analysis of podoplanin expression in mouse lymph tissue extract. -SCBT QC
Date published: 2015-03-23
Rated 5 out of 5 by from IHC with murine lung tissue extractsIHC with murine lung tissue extracts. -SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from Positive results in western blot using mouse lymphPositive results in western blot using mouse lymph, mouse kidney and mouse thymus tissue extracts under reducing and non-reducing conditions. -SCBT QC
Date published: 2013-10-02
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podoplanin 抗体 (8.1.1) is rated 5.0 out of 5 by 8.
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