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Waf1/Cip1/CDKN1A p21 Antibody (F-8) - Western Blotting - Image 134781
p21 Waf1/Cip1 抗体 (F-8): sc-271610. 未转染 293T: sc-117752 (A), 小鼠 p21 Waf1/Cip1 转染 293T: sc-122305 (B) 和 C32 (C) 全细胞裂解液中 p21 Waf1/Cip1 表达的 Western 印迹分析.
p21 Waf1/Cip1 抗体 (F-8): sc-271610. 未转染 293T: sc-117752 (A), 小鼠 p21 Waf1/Cip1 转染 293T: sc-122305 (B) 和 C32 (C) 全细胞裂解液中 p21 Waf1/Cip1 表达的 Western 印迹分析.

Waf1/Cip1/CDKN1A p21 抗体 (F-8): sc-271610

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说明书
  • Waf1/Cip1/CDKN1A p21抗体(F-8)是小鼠单克隆IgG2a κ, 在41篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的p21的C-terminus的氨基酸124-164之间
  • 抗-Waf1/Cip1/CDKN1A p21 抗体 (F-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 p21 Waf1/Cip1; 也和以下物种反应，包括： 和 canine and porcine
  • 抗Waf1/Cip1/CDKN1A p21抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Waf1/Cip1/CDKN1A p21 (F-8): sc-271610的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Waf1/Cip1/CDKN1A p21 Antibody (F-8) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Waf1/Cip1/CDKN1A p21 Antibody (F-8) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

p21 Waf1/Cip1/CDKN1A抗体（F-8）是一种IgG2a κ小鼠单克隆p21 Waf1/Cip1抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的p21 Waf1/Cip1。p21 Waf1/Cip1/CDKN1A抗体（F-8）既可以是非偶联的抗p21 Waf1/Cip1抗体形式，也可以是多种偶联形式的抗p21 Waf1/Cip1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。现在已经明确，细胞周期蛋白通过它们与相应的细胞周期蛋白依赖性激酶（Cdks）结合并激活的能力，在促进细胞周期转变中发挥积极作用。Cdk2与细胞周期蛋白A、D和E结合，并与哺乳动物中G1期向S期转变的控制有关。在细胞周期蛋白A、细胞周期蛋白D1、细胞周期蛋白E和Cdk2免疫沉淀物中，已发现一种新型的Cdk相互作用蛋白，称为p21 Waf1/Cip1、Cip1或WAF1。p21 Waf1/Cip1是Cdks的强效紧密结合抑制剂，可通过cyclin a - cdk2、cyclin E-Cdk2、cyclin D1-Cdk4和cyclin D2-Cdk4复合物抑制Rb的磷酸化。p21 Waf1/Cip1的表达可被野生型而非突变型p53诱导。小鼠的p21 Waf1/Cip1同源物被称为CAP20。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Waf1/Cip1/CDKN1A p21 抗体 (F-8) 参考文献:

  1. p21（WAF1/CIP1）表达的改变与肝外胆管癌预后不良有关。  |  Li, X., et al. 2000. Cancer Lett. 154: 85-91. PMID: 10799743
  2. 低、高格里森评分前列腺癌中p21(waf1/cip1)、p27 (kip1)、p63和雄激素受体的表达。  |  Romics, I., et al. 2008. Pathol Oncol Res. 14: 307-11. PMID: 18415709
  3. 在人类肝癌HLE细胞中，gnidimacrin通过诱导p21(WAF1/Cip1)和阻遏cdc2来阻止G2期。  |  Yoshida, M., et al. 2009. Anticancer Res. 29: 1349-54. PMID: 19414386
  4. 多种microRNA通过抑制p21(Waf1/Cip1)对Ras诱导的衰老起保护作用。  |  Borgdorff, V., et al. 2010. Oncogene. 29: 2262-71. PMID: 20101223
  5. Zac1是一种类似于Sp1的蛋白，它能调节HeLa细胞中人类p21（WAF1/Cip1）基因的表达。  |  Liu, PY., et al. 2011. Exp Cell Res. 317: 2925-37. PMID: 22001409
  6. HIF-2α/ARNT复合物通过诱导p21(Waf1/Cip1)和p27(Kip1)来调节毛发生长。  |  Imamura, Y., et al. 2014. FASEB J. 28: 2517-24. PMID: 24599965
  7. 阿霉素通过上调p21（Waf1/Cip1）和C/EBPα的表达来激活乙型肝炎病毒的复制。  |  Chen, YF., et al. 2015. PLoS One. 10: e0131743. PMID: 26121644
  8. p21(WAF1/CIP1)的C端结构与人类PCNA结合。  |  Gulbis, JM., et al. 1996. Cell. 87: 297-306. PMID: 8861913
  9. 在HL-60细胞分化过程中，肿瘤坏死因子α诱导p21(WAF1/CIP1)的表达，而p53并不参与这一过程。  |  Yoshida, K., et al. 1996. Int J Hematol. 65: 41-8. PMID: 8990624
  10. 人类肾上腺皮质腺瘤中p21(Waf1/Cip1)基因的体细胞突变。  |  Iida, S., et al. 1997. Anticancer Res. 17: 633-6. PMID: 9066592

订购信息

产品名称产品编号规格价格数量收藏夹

Waf1/Cip1/CDKN1A p21 抗体 (F-8)

sc-271610
200 µg/ml
$316.00

Waf1/Cip1/CDKN1A p21 (F-8): m-IgG Fc BP-HRP 套装

sc-529221
200 µg Ab; 10 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 (F-8): m-IgGκ BP-HRP 套装

sc-521996
200 µg Ab, 40 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) AC

sc-271610 AC
500 µg/ml, 25% agarose
$416.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) HRP

sc-271610 HRP
200 µg/ml
$316.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) FITC

sc-271610 FITC
200 µg/ml
$330.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) PE

sc-271610 PE
200 µg/ml
$343.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) Alexa Fluor® 488

sc-271610 AF488
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) Alexa Fluor® 546

sc-271610 AF546
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) Alexa Fluor® 594

sc-271610 AF594
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) Alexa Fluor® 647

sc-271610 AF647
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) Alexa Fluor® 680

sc-271610 AF680
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 抗体 (F-8) Alexa Fluor® 790

sc-271610 AF790
200 µg/ml
$357.00

Waf1/Cip1/CDKN1A p21 (F-8) 中和勝肽

sc-271610 P
100 µg/0.5 ml
$68.00

What is the best secondary to use with your mouse monoclonal antibodies for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 5 out of 5 by from Worked excellent on our western blotsWe used it 1:1000 on our immunoblots. The signals were excellent.
Date published: 2021-07-29
Rated 1 out of 5 by from very bad productit is not working in cell lysate especially human cell lines (hepG2)
Date published: 2020-10-22
Rated 5 out of 5 by from Great antibody!Used at 1:200 for mouse tissue lysates. Antibody gave good clear band! Recommend!
Date published: 2018-03-23
Rated 5 out of 5 by from very strong signalSpecific WB band in HeLa whole cell lysate, 1:300 dilution, Good data！！！
Date published: 2017-04-05
Rated 5 out of 5 by from Strong and Specificp21 (F-8) gives a strong specific band for Western blot (WB) at 21 kDa.
Date published: 2016-12-20
Rated 5 out of 5 by from great1:1000 dilution for detection of p21 in DLBCL lymphoma cells, incubate with the filter overnight at 4C. It's very sensitive and stable with the clean background.
Date published: 2016-12-17
Rated 4 out of 5 by from This has worked well in WB with mouse liverThis has worked well in WB with mouse liver.
Date published: 2015-12-07
Rated 5 out of 5 by from This has worked well in WB with mouse liverThis has worked well in WB with mouse liver
Date published: 2015-10-16
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Waf1/Cip1/CDKN1A p21 抗体 (F-8) is rated 4.5 out of 5 by 13.
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