Date published: 2025-9-9

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Waf1/Cip1/CDKN1A p21 抗体 (AC8.3): sc-53393

4.5(2)
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说明书
  • Waf1/Cip1/CDKN1A p21抗体(AC8.3)是小鼠单克隆IgG1 κ, 在4篇文献中引用,规格为200 µg/ml
  • 针对His标签的human p21 Waf1/Cip1表达在杆状病毒中
  • 推荐用于 human 来源的p21 Waf1/Cip1 WB, IP, IF 和 IHC(P)检测
  • 看 Waf1/Cip1/CDKN1A p21 (F-5): sc-6246,可查看有标记的 Waf1/Cip1/CDKN1A p21 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Waf1/Cip1/CDKN1A p21 抗体 (AC8.3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Waf1/Cip1/CDKN1A p21 抗体 (AC8.3) 组合成套装提供。 (请参阅下面的订购信息)。

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目前已确定,细胞周期蛋白通过与同源细胞周期蛋白依赖激酶(Cdks)结合并激活,在促进细胞周期转换中发挥积极作用。Cdk2与细胞周期蛋白A, D和E结合,并与哺乳动物中G1期向S期转变的控制有关。一个新的cdk相互作用蛋白,命名为p21 Waf1/Cip1, Cip1或Waf1,已在cyclin A, cyclin D1, cyclin E和Cdk2免疫沉淀中被鉴定。p21 Waf1/Cip1是一种有效的Cdks紧密结合抑制剂,可通过cyclin a - cdk2、cyclin E-Cdk2、cyclin D1-Cdk4和cyclin D2-Cdk4复合物抑制Rb的磷酸化。野生型p53可诱导p21 Waf1/Cip1的表达,而突变型p53则不能。p21 Waf1/Cip1的小鼠同源物命名为CAP20。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Waf1/Cip1/CDKN1A p21 抗体 (AC8.3)

sc-53393
200 µg/ml
$316.00

Waf1/Cip1/CDKN1A p21 (AC8.3): m-IgG Fc BP-HRP 套装

sc-536953
200 µg Ab; 10 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 (AC8.3): m-IgGκ BP-HRP 套装

sc-534130
200 µg Ab; 40 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 (AC8.3): m-IgG1 BP-HRP 套装

sc-544919
200 µg Ab; 20 µg BP
$354.00

Can p21 (AC8.3): sc-53393 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, p21 (AC8.3): sc-53393 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. This particular p21 antibody is not the best choice for IHC. Instead, p21 (F-5): sc-6246 is a directly conjugated mouse monoclonal antibody that can be easily used for IHC. It has many possible conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 5 out of 5 by from Good nuclear and cytoplasmic staining withGood nuclear and cytoplasmic staining with paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-02-11
Rated 4 out of 5 by from Work well with some background in MCF7Work well with some background in MCF7, C32 and phorbol-treated C32 nuclear extracts. -SCBT QC
Date published: 2014-07-08
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Waf1/Cip1/CDKN1A p21 抗体 (AC8.3) is rated 4.5 out of 5 by 2.
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