Date published: 2025-9-6
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p-ERK Antibody (E-4) - Western Blotting - Image 383055
p-ERK 抗体 (E-4): sc-7383. 对 HeLa (A), A-431 (B), SK-MEL-24 (C), Jurkat (D), K-562 (E) 和 NIH/3T3 (F) 全细胞裂解液中 ERK 磷酸化的 Western 印迹分析.
p-ERK 抗体 (E-4): sc-7383. 对 HeLa (A), A-431 (B), SK-MEL-24 (C), Jurkat (D), K-562 (E) 和 NIH/3T3 (F) 全细胞裂解液中 ERK 磷酸化的 Western 印迹分析.

p-ERK 抗体 (E-4): sc-7383

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说明书
  • p-ERK抗体(E-4)是小鼠单克隆IgG2a κ, 在3,292篇文献中引用，规格为200 µg/ml
  • p-ERK 抗体 (E-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗p-ERK抗体（E-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 以及 ELISA;偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取p-ERK (E-4): sc-7383的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 p-ERK 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-ERK 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加ERK抗体，包括ERK 1, ERK 2, ERK 3和ERK 5

p-ERK抗体（E-4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附 酶联免疫吸附试验（ELISA）。p-ERK 单克隆抗体（E-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。ERK蛋白（包括ERK 1和ERK 2）在生长因子、激素和神经递质引发的信号转导通路中起着关键作用。ERK蛋白位于细胞质中，激活后转移到细胞核，在那里调节基因表达并影响细胞增殖、分化及存活。ERK在特定的苏氨酸和酪氨酸残基上的双重磷酸化对于酶的完全激活至关重要，从而允许丝氨酸和苏氨酸残基上的下游靶标磷酸化。ERK的主要上游调节因子包括MAP激酶激酶（MEK）、MEK激酶和Raf-1，它们对于MAPK信号级联的正确运作至关重要。此外，ERK家族还包括其他三个成员： ERK 3、ERK 5和ERK 6，它们分别参与不同的细胞过程。抗ERK抗体（E-4）是研究ERK在Tyr 204位点磷酸化的重要工具，该位点磷酸化是激活和MAPK信号级联反应的关键修饰。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-ERK 抗体 (E-4) 参考文献:

  1. ERK1和ERK2是两种微管相关蛋白2激酶，它们在原位介导酪氨酸羟化酶丝氨酸-31的磷酸化。  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
  2. 纯化磷酸化并激活 Erk-1 基因产物的鼠蛋白酪氨酸/苏氨酸激酶：与裂殖酵母 byr1 基因产物的关系。  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
  3. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  4. 细胞外信号调节激酶 1（一种胰岛素刺激的微管相关蛋白 2 激酶）的纯化和特性。  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
  5. 确定 pp42/丝裂原活化蛋白激酶（MAP 激酶）的调节磷酸化位点。  |  Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
  6. ERK：丝氨酸/苏氨酸蛋白激酶家族，在胰岛素和 NGF 的作用下被激活并酪氨酸磷酸化。  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
  7. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  8. 参与 C2C12 肌母细胞分化的有丝分裂原激活蛋白激酶 ERK6  |  Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070

订购信息

产品名称产品编号规格价格数量收藏夹

p-ERK 抗体 (E-4)

sc-7383
200 µg/ml
$316.00

p-ERK (E-4): m-IgG Fc BP-HRP 套装

sc-525440
200 µg Ab; 10 µg BP
$354.00

p-ERK (E-4): m-IgG2a BP-HRP 套装

sc-546255
200 µg Ab; 10 µg BP
$354.00

p-ERK 抗体 (E-4) AC

sc-7383 AC
500 µg/ml, 25% agarose
$416.00

p-ERK 抗体 (E-4) HRP

sc-7383 HRP
200 µg/ml
$316.00

p-ERK 抗体 (E-4) FITC

sc-7383 FITC
200 µg/ml
$330.00

p-ERK 抗体 (E-4) PE

sc-7383 PE
200 µg/ml
$343.00

p-ERK 抗体 (E-4) Alexa Fluor® 488

sc-7383 AF488
200 µg/ml
$357.00

p-ERK 抗体 (E-4) Alexa Fluor® 546

sc-7383 AF546
200 µg/ml
$357.00

p-ERK 抗体 (E-4) Alexa Fluor® 594

sc-7383 AF594
200 µg/ml
$357.00

p-ERK 抗体 (E-4) Alexa Fluor® 647

sc-7383 AF647
200 µg/ml
$357.00

p-ERK 抗体 (E-4) Alexa Fluor® 680

sc-7383 AF680
200 µg/ml
$357.00

p-ERK 抗体 (E-4) Alexa Fluor® 790

sc-7383 AF790
200 µg/ml
$357.00

p-ERK 抗体 (E-4) B

sc-7383 B
200 µg/ml
$320.00

p-ERK 抗体 (E-4) TRITC

sc-7383 TRITC
200 µg/ml
$320.00

p-ERK 抗体 (E-4) Alexa Fluor® 405

sc-7383 AF405
200 µg/ml
$357.00

p-ERK (E-4) 中和勝肽

sc-7383 P
100 µg/0.5 ml
$68.00

What is the amino acid sequence of the blocking peptide (sc-7383 P)?

Asked by: tarotaro27
Thank you for your question. This information is proprietary. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2022-03-10

I am writing to ask if your Alexafluor-labeled p-ERK antibody, cat# sc-7383 produces a good immunofluorescent signal, and do you have any images you'd be willing to share ? I'd like to see the staining pattern before I purchase it...many thanks !!!!

Asked by: Anonymous
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2024-11-01

Hello, I work in lab at UCSC and was wondering if I could obtain a sample of this p-ERK antibody (sc-7383)? Thank you, Celine

Asked by: cneudorf
Thank you for your question. Please contact our Technical Service Department directly to arrange a free 10 µg sample of p-ERK (E-4): sc-7383.
Answered by: Tech Support Europe
Date published: 2021-01-08

Hello Sir/Mam Should we have to use 5% BSA as the blocking agent, diluent for primary antibody and secondary antibody or is 5% milk okay to use.

Asked by: Neha attal
Thank you for your question. We recommend using our plant protein-based UltraCruz® Blocking Reagent: sc-516214 for blocking purposes. BSA- or milk powder-based may also be used instead: If signal strength is too low, BSA may be the better choice, whereas a milk powder blocking buffer is better suited to block promiscuous, unspecific signals.
Answered by: Tech Support Europe
Date published: 2020-06-30

Does sc-7383 recognize dually phosphorylated ERK1/2? What is the biological role of mono-phosphorylated ERK1/2 and does it necessarily undergo threonine phosphorylation subsequently? Thank you.

Asked by: Biologist
Thank you for the question. p-ERK Antibody (E-4): sc-7383 is recommended for detection of ERK 1 phosphorylated Tyr at the position 204 with a mw of ~44kDa. Correspondingly phosphorylated ERK 2 will also be detected at ~42kDa. It was not examined if the antibody has a different binding capacity of the dually phosphorylated vs. the mono phosphorylated ERK proteins. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Regarding additional pathway information I suggest checking the protein info https://www.ncbi.nlm.nih.gov/protein/P27361 and the available product citations and background references listed for this product.
Answered by: Technical Support Europe
Date published: 2018-02-01

Hi, Officer: May I ask a FREE 10 µg sample of p-ERK (E-4): sc-7383 for my Experiment ? Thanks!!!!!!!!!!

Asked by: Ling Sun
Thank you for your question. Yes, for a limited time we are offering a free 10 µg sample of our mouse monoclonal antibody, p-ERK (E-4): sc-7383. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website
Answered by: Technical Support
Date published: 2017-06-12

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anonymous
Yes. We recommend using a directly conjugated version of p-ERK Antibody (E-4) sc-7383. This antibody is currently available conjugated to AlexaFluor 405 (sc-7383 AF405), AlexaFluor 488 (sc-7383 AF488), AlexaFluor 647 (sc-7383 AF647), biotin (sc-7383 B), fluorescein (sc-7383 FITC), HRP (sc-7383 HRP), PCPC5 (sc-7383 PCPC5), phycoerythrin (sc-7383 PE), PerCP (sc-7383 PerCP), and TRITC (sc-7383 TRITC).
Answered by: Tech Service 6
Date published: 2016-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anonymous
We recommend using one our Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-14
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Rated 5 out of 5 by from Western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-01
Rated 5 out of 5 by from The antibody has good qualityFew months ago, we used the antibody to assay the activation of ERK in mouse liver tissue. It worked very well.
Date published: 2024-01-02
Rated 5 out of 5 by from Very goodVery good antibody for detection of phospho ERK in western blot
Date published: 2023-12-12
Rated 4 out of 5 by from Good for WBWork well for western blot analysis in rat renal tubular cells
Date published: 2023-08-18
Rated 4 out of 5 by from sc-7383 p-ERKWe use it to detect p-ERK in HUVEC cells with western blot.
Date published: 2023-07-07
Rated 3 out of 5 by from Other Bands of p-ERKI did this antibody on mouse heart with loading 45 microgram protein, I got strong signal around 30 but not at the mentioned molecular weight. I need to know the reason for that.
Date published: 2022-02-23
Rated 5 out of 5 by from Accurate and reproducibleThis product is excellent, just as the ones I previously bought.
Date published: 2021-08-27
Rated 5 out of 5 by from Great features!his primary antibody was able to detect phosphorylated ERK (p42 and p44) giving well defined bands.
Date published: 2021-07-08
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p-ERK 抗体 (E-4) is rated 4.7 out of 5 by 101.
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