Date published: 2025-9-6
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Nurr1 Antibody (F-5) - Western Blotting - Image 352038
Nurr1 抗体 (F-5): sc-376984. A-431 (A), SK-N-MC (B), HeLa (C) 和 Jurkat (D) 全细胞裂解液中 Nurr1 表达的 Western 印迹分析.
Nurr1 抗体 (F-5): sc-376984. A-431 (A), SK-N-MC (B), HeLa (C) 和 Jurkat (D) 全细胞裂解液中 Nurr1 表达的 Western 印迹分析.

Nurr1 抗体 (F-5): sc-376984

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寫評論提問

说明书
  • Nurr1 抗体 F-5 是小鼠单克隆 IgG2a κ，Nurr1抗体, 在19篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的Nurr1的氨基酸66-262
  • Nurr1 抗体 (F-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Nurr1
  • 抗Nurr1抗体（F-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-376984 X, 200 µg/0.1 ml)
  • 关于如何获取Nurr1 (F-5): sc-376984的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是Nurr1 Antibody (F-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Nurr1 Antibody (F-5) 打包提供（请参阅下面的订购信息）。

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说明书与实验方案
研究信息

関連項目

额外Nurr抗体，包括Nurr1

Nurr1抗体（F-5）是一种IgG2a κ小鼠单克隆Nurr1抗体（也称为NR4A2抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的Nurr1蛋白。Nurr1抗体（F-5）既可作为非偶联的抗Nurr1抗体形式提供，也可作为多种偶联形式的抗Nurr1抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。Nurr1（Nur相关因子1）和Nur77（也称为NGFI-B）编码孤儿核受体，这些受体可能构成核受体超家族中的一个额外亚家族。小鼠Nurr1对应的大鼠和人类同源物分别被命名为RNR1和NOT。Nurr1和Nur77都是生长因子可诱导的、即时早期反应基因。在膜去极化后可见Nurr1和Nur77的诱导，而仅在用NGF刺激时可见Nur77的诱导。JunD充当Nur77的介体。在G0到G1过渡期间和整个G1阶段，活化T细胞中可见Nurr77表达的增加。除了作为即时早期基因的功能外，Nur77还可能在TCR介导的凋亡中发挥作用。环孢菌素A是一种强效免疫抑制剂，已显示可抑制Nur77与DNA结合的能力。Nur77的显性负性形式可以保护T细胞杂交瘤免受激活诱导的凋亡。但是，对于TCR介导的凋亡而言，Nur77的绝对需求仍在争论中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nurr1 抗体 (F-5) 参考文献:

  1. 鉴定新的脑特异性转录因子 NURR1。  |  Law, SW., et al. 1992. Mol Endocrinol. 6: 2129-35. PMID: 1491694
  2. Nurr1，一种在多巴胺细胞发育中发挥关键作用的孤儿核受体。  |  Perlmann, T. and Wallén-Mackenzie, A. 2004. Cell Tissue Res. 318: 45-52. PMID: 15340833
  3. 小鼠大脑中典型和非典型抗精神病药物对nur家族转录因子（nurr1, nur77和nor-1）的诱导模式：对其作用机制的影响。  |  Maheux, J., et al. 2005. J Pharmacol Exp Ther. 313: 460-73. PMID: 15615863
  4. NGFI-B 家族在肾上腺分区和肾上腺皮质疾病中的作用。  |  Bassett, MH., et al. 2004. Endocr Res. 30: 567-74. PMID: 15666793
  5. Nurr1作为阿尔茨海默病相关发病机理的介体和治疗靶点的关键作用。  |  Jeon, SG., et al. 2020. Aging Dis. 11: 705-724. PMID: 32489714
  6. Nurr1调节剂的药物化学和化学生物学：神经退行性疾病的新兴策略。  |  Willems, S. and Merk, D. 2022. J Med Chem. 65: 9548-9563. PMID: 35797147
  7. 缺乏核受体 NGFI-B (Nur77) 的小鼠胸腺和外周 T 细胞死亡不受影响。  |  Lee, SL., et al. 1995. Science. 269: 532-5. PMID: 7624775
  8. NOT，一种与类固醇/甲状腺激素受体 NAK1/TR3 密切相关的人类即时早期反应基因。  |  Mages, HW., et al. 1994. Mol Endocrinol. 8: 1583-91. PMID: 7877627
  9. T 细胞活化过程中 NGFI-B 基因表达的诱导。蛋白磷酸酶的作用  |  Garcia, I., et al. 1994. J Immunol. 153: 3417-25. PMID: 7930567
  10. JunD 参与了 PC12 细胞中神经生长因子和膜去极化对孤儿受体基因 nur77 的转录激活。  |  Yoon, JK. and Lau, LF. 1994. Mol Cell Biol. 14: 7731-43. PMID: 7969116
  11. Nur77 孤儿类固醇受体在细胞凋亡中的分子特征。  |  Winoto, A. 1994. Int Arch Allergy Immunol. 105: 344-6. PMID: 7981602
  12. 肾上腺中孤儿核受体 Nur77 和 Nurr-1 的内分泌和神经源调控。  |  Davis, IJ. and Lau, LF. 1994. Mol Cell Biol. 14: 3469-83. PMID: 8164692

订购信息

产品名称产品编号规格价格数量收藏夹

Nurr1 抗体 (F-5)

sc-376984
200 µg/ml
$316.00

Nurr1 (F-5): m-IgG Fc BP-HRP 套装

sc-526071
200 µg Ab; 10 µg BP
$354.00

Nurr1 (F-5): m-IgG2a BP-HRP 套装

sc-546495
200 µg Ab; 10 µg BP
$354.00

Nurr1 抗体 (F-5) X

sc-376984 X
200 µg/0.1 ml
$316.00

Nurr1 抗体 (F-5) AC

sc-376984 AC
500 µg/ml, 25% agarose
$416.00

Nurr1 抗体 (F-5) HRP

sc-376984 HRP
200 µg/ml
$316.00

Nurr1 抗体 (F-5) FITC

sc-376984 FITC
200 µg/ml
$330.00

Nurr1 抗体 (F-5) PE

sc-376984 PE
200 µg/ml
$343.00

Nurr1 抗体 (F-5) Alexa Fluor® 488

sc-376984 AF488
200 µg/ml
$357.00

Nurr1 抗体 (F-5) Alexa Fluor® 546

sc-376984 AF546
200 µg/ml
$357.00

Nurr1 抗体 (F-5) Alexa Fluor® 594

sc-376984 AF594
200 µg/ml
$357.00

Nurr1 抗体 (F-5) Alexa Fluor® 647

sc-376984 AF647
200 µg/ml
$357.00

Nurr1 抗体 (F-5) Alexa Fluor® 680

sc-376984 AF680
200 µg/ml
$357.00

Nurr1 抗体 (F-5) Alexa Fluor® 790

sc-376984 AF790
200 µg/ml
$357.00

How is this antibody purified?  Has it been tested in IHC on mouse tissue?

Asked by: Mixer
Thank you for your question. Nurr1 (F-5): sc-376984 is an affinity purified mouse monoclonal antibody. This antibody has not yet been tested for IHC on mouse tissue. We currently recommend and warranty the antibody for IF, or immunofluorescent staining of fresh or frozen tissues or cells fixed in acetone or methanol. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-05-18

Is this antibody suitable for ChIP with magnetic beads? Thank you.

Asked by: La_B
Thank you for your question. We are not aware of any publications citing use of this Nurr1 Antibody (F-5): sc-376984 for chromatin immunoprecipitation or ChIP. However, we do offer a more concentrated version of the antibody, TransCruz reagent that is warrantied for use in a ChIP application (sc-376984 X, 200 µg/0.1 ml). If you purchase the antibody and it does not work for you in ChIP, then please contact our Technical Service Department and we can assist you with troubleshooting the antibody, or offering you a replacement or a refund.
Answered by: Technical Support
Date published: 2022-06-20

When should the TransCruz reagent sc-376984 X be used instead of the standard form sc-376984?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 3 out of 5 by from Good for IF stainingIF (1:50) work well on frozen section from adult mouse brain tissue. Pre-permeabilization in PB-Triton-X 0.5% for 45 mins. Blocking BSA 2% in PB-Triton-X 0.5%. Primary (4°C for 2 nights).
Date published: 2021-06-17
Rated 4 out of 5 by from Good for IFIF (1:200 with antigen retrieval) work well on frozen section from embryonic mouse brian tissue.
Date published: 2019-01-02
Rated 4 out of 5 by from Sehr gute Banden in WBHabe den Antikörper in Probengröße erhalten (als Ersatz für den nicht mehr verfügbaren E-20) und bin sehr zufrieden. Sehr sensitiv in WB und erste Tests in IF sehen auch ordentlich aus.
Date published: 2018-07-19
Rated 4 out of 5 by from Very good antibodyI got a free sample of NURR1 antibody and believe me that it is working awesome. I am in the mood of purchasing a full vial soon.
Date published: 2018-03-29
Rated 1 out of 5 by from ne fonctionne pas sur la sourisNe fonctionne pas en WB sur des extraits de surrénales de souris (aucune bande) ni en immunohistochimie sur des surrénales de souris
Date published: 2017-02-02
Rated 5 out of 5 by from Strong Nurr1 ab for WBGreat clear bands in A-431, SK-N-MC, HeLa and Jurkat whole cell lysates.
Date published: 2016-12-21
Rated 5 out of 5 by from Produced positive Western blot data of Nurr1Produced positive Western blot data of Nurr1 expression in SW-13 and SK-N-MC whole cell lysates. -SCBT QC
Date published: 2014-06-11
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Nurr1 抗体 (F-5) is rated 3.7 out of 5 by 7.
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