Date published: 2025-9-7
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Keap1 Antibody (G-2) - Western Blotting - Image 143387
Keap1 抗体 (G-2): sc-365626. Western 印迹分析 Keap1 在 Hep G2 全细胞裂解液中的表达.
Keap1 抗体 (G-2): sc-365626. Western 印迹分析 Keap1 在 Hep G2 全细胞裂解液中的表达.

Keap1 抗体 (G-2): sc-365626

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说明书
  • Keap1 抗体 G-2 是小鼠单克隆 IgG2b κ，Keap1抗体, 在90篇文献中引用，规格为200 µg/ml
  • 针对human起源的Keap1内部区域内的氨基酸181-370进行
  • Keap1 抗体 (G-2) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Keap1
  • 抗Keap1抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Keap1 (G-2): sc-365626的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Keap1 Antibody (G-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Keap1 Antibody (G-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Keap1抗体（G-2）是一种IgG2b κ小鼠单克隆Keap1抗体（也称为KEAP1抗体，KLHL19抗体），可通过WB、IP、IF和ELISA检测人源Keap1蛋白。Keap1抗体（G-2）有非偶联抗Keap1抗体形式和多种偶联抗Keap1抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Keap1（kelch样ECH相关蛋白1，INrf2，KLHL19）是Cullin3（Cul3）依赖性E3泛素连接酶复合物的应激感应接头，负调控Nrf2（NF-E2相关因子2）。蛋白质的稳态水平受泛素途径的影响，泛素途径由泛素激活（E1）、偶联（E2）和连接（E3）组成。Keap1与Cul3和Rbx1组装成E3泛素连接酶复合物，靶向Nrf2 N端Neh2结构域中的赖氨酸残基进行泛素偶联。Keap1-Nrf2系统介导氧化应激和/或亲电应激下的细胞保护基因表达。Keap1在非应激条件下持续抑制Nrf2活性，氧化剂或亲电试剂会引发Keap1活性的抑制，诱导Nrf2活化。Keap1的Cys 273和Cys 288残基是抑制Nrf2核积聚所必需的。Keap1通过活性Crm1/exportin依赖的核输出机制将Nrf2在细胞质中隔离。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Keap1 抗体 (G-2) 参考文献:

  1. Keap1中不同的半胱氨酸残基是Keap1依赖性泛素化Nrf2以及化学预防剂和氧化应激稳定Nrf2所必需的。  |  Zhang, DD. and Hannink, M. 2003. Mol Cell Biol. 23: 8137-51. PMID: 14585973
  2. 氧化应激传感器Keap1作为基于Cul3的E3连接酶的适配器，调节Nrf2的蛋白酶体降解。  |  Kobayashi, A., et al. 2004. Mol Cell Biol. 24: 7130-9. PMID: 15282312
  3. Keap1 是依赖于 Cul3 的泛素连接酶复合物的氧化还原调节底物适配蛋白。  |  Zhang, DD., et al. 2004. Mol Cell Biol. 24: 10941-53. PMID: 15572695
  4. 将针对 Keap1 的 siRNA 作为激发癌症化学预防表型的一种策略。  |  Devling, TW., et al. 2005. Proc Natl Acad Sci U S A. 102: 7280-7285A. PMID: 15883370
  5. Keap1通过一种依赖于Crm1的核输出机制调节Nrf2进出细胞核的氧化敏感性。  |  Velichkova, M. and Hasson, T. 2005. Mol Cell Biol. 25: 4501-13. PMID: 15899855
  6. 内皮细胞中 Nrf2-Keap1 系统对层状和振荡剪切应力的不同反应。  |  Hosoya, T., et al. 2005. J Biol Chem. 280: 27244-50. PMID: 15917255
  7. 特定的亲电子吸附模式会触发 Keap1 泛素化和 Nrf2 激活。  |  Hong, F., et al. 2005. J Biol Chem. 280: 31768-75. PMID: 15985429
  8. Nrf2 通过涉及 Keap1 的核胞质穿梭的动力学途径控制 ARE 驱动基因的组成型和诱导型表达。  |  Nguyen, T., et al. 2005. J Biol Chem. 280: 32485-92. PMID: 16000310
  9. KEAP1-NRF2系统：一种基于硫醇的传感器-效应器装置，用于维持氧化还原平衡。  |  Yamamoto, M., et al. 2018. Physiol Rev. 98: 1169-1203. PMID: 29717933
  10. 多糖通过增加 miR-200a 来调节 Keap1/Nrf2 通路，从而预防果糖诱导的肝脏炎症和脂质沉积。  |  Zhao, XJ., et al. 2018. Redox Biol. 18: 124-137. PMID: 30014902
  11. Keap1/Nrf2/ARE信号通路在慢性疾病中的抗炎和抗氧化机制。  |  Tu, W., et al. 2019. Aging Dis. 10: 637-651. PMID: 31165007
  12. Keap1-Nrf2相互作用的小分子抑制剂通过选择性增强感染部位巨噬细胞的抗菌防御能力来减轻败血症。  |  Wang, Y., et al. 2023. EBioMedicine. 90: 104480. PMID: 36863256

订购信息

产品名称产品编号规格价格数量收藏夹

Keap1 抗体 (G-2)

sc-365626
200 µg/ml
$316.00

Keap1 (G-2): m-IgGκ BP-HRP 套装

sc-522298
200 µg Ab, 40 µg BP
$354.00

Keap1 (G-2): m-IgG2b BP-HRP 套装

sc-549019
200 µg Ab; 10 µg BP
$354.00

Keap1 抗体 (G-2) AC

sc-365626 AC
500 µg/ml, 25% agarose
$416.00

Keap1 抗体 (G-2) HRP

sc-365626 HRP
200 µg/ml
$316.00

Keap1 抗体 (G-2) FITC

sc-365626 FITC
200 µg/ml
$330.00

Keap1 抗体 (G-2) PE

sc-365626 PE
200 µg/ml
$343.00

Keap1 抗体 (G-2) Alexa Fluor® 488

sc-365626 AF488
200 µg/ml
$357.00

Keap1 抗体 (G-2) Alexa Fluor® 546

sc-365626 AF546
200 µg/ml
$357.00

Keap1 抗体 (G-2) Alexa Fluor® 594

sc-365626 AF594
200 µg/ml
$357.00

Keap1 抗体 (G-2) Alexa Fluor® 647

sc-365626 AF647
200 µg/ml
$357.00

Keap1 抗体 (G-2) Alexa Fluor® 680

sc-365626 AF680
200 µg/ml
$357.00

Keap1 抗体 (G-2) Alexa Fluor® 790

sc-365626 AF790
200 µg/ml
$357.00

Sie bieten 3 monoklonale anti-KEAP1 Antikörper an. Unmarkiert bieten Sie diese an mit der Angabe 200 µg/mL. Mir ist aber nicht klar, welches Volumen das Gebinde hat bzw. wieviel µg absolut das entspricht. Bitte um Ihre Auskunft dazu.

Asked by: JStr
Vielen Dank für Ihre Anfrage. Der Antikörper wird als 200 µg in 1 ml PBS mit 0.1 % Gelatine und <0.1 % Natriumazid geliefert. This antibody is supplied as 200 µg in 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Answered by: Tech Support Europe
Date published: 2024-10-24

Would this antibody be suitable for IF on human lung tissue sections?

Asked by: Anonymous
Thank you for your question. sc-365626 has been validated to work on human samples and for the following applications . WB, IP, IF, ELISA
Answered by: BlakeJ
Date published: 2023-03-16

There are two close bands at the expected MW position. Are they both keap1 with different status or one just non-specific binding?

Asked by: PeterCH
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-12-03

My membrane has been treated with Keap1 (G-2) HRP antibody, Can I directly use the chemoluminescence kit? Thanks!

Asked by: RemiFromGeneva
Thank you for your question. If you are using an HRP-conjugated primary antibody, no secondary detection antibody is required. You can use conventional HRP-based chemoluminescence to detect the primary antibody on your membrane. Please also refer to D. Immunoblotting For Direct Detection in our recommended western blotting protocol. You can find the protocol under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2021-08-16

Hello, What secondary antibody can I use with Keap1 Antibody (G-2): sc-365626? It is a IgG2b (kappa light chain) antibody

Asked by: Carlos LG
Thank you for your question. Keap1 Antibody (G-2) is a mouse monoclonal IgG2b (kappa light chain). It can be detected by any of our Anti-Mouse IgG Kappa Binding Protein Secondaries, such as m-IgGκ BP-HRP: sc-516102, m-IgGκ BP-CFL 488: sc-516176, or m-IgGκ BP-CFL 680: sc-516180. If you require additional information, do not hesitate to contact our technical service.
Answered by: Technical Support Europe
Date published: 2019-04-01

What is the best Keap1 antibody for Spargue Dawley Rats in brain and muscle

Asked by: Allan
Thank you for your question. For rat samples I would suggest sc-514914, although both sc-514914 and sc-515432 are recommended for rat use.
Answered by: Technical Support
Date published: 2019-01-26

Can this antibody be used for mouse cells?

Asked by: Celine0123
Thank you for your question. We have not yet tested this antibody, Keap1 (G-2): sc-365626, for use in mouse cells, so it is not currently recommended for that species. We would recommend an alternative antibody for detection of Keap1 in mouse samples, Keap1 (A-4): sc-515432. A link to the product information for this antibody is provided below for your reference. https://www.scbt.com/scbt/product/keap1-antibody-a-4
Answered by: Technical Support
Date published: 2017-07-24

Can the (G-2) clone be used for use in immunohistochemistry of formalin-fixed paraffin-embedded tissue sections?

Asked by: cjMara
This antibody, Keap1 (G-2): sc-365626, has not yet been tested for IHC(P). Instead we would strongly recommend an alternate clone, Keap1 (F-10): sc-514914, which has excellent data in FFPE human tissues.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from Great features for western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-05
Rated 5 out of 5 by from Great for WBThis primary antibody is useful in western blotting.
Date published: 2024-03-21
Rated 4 out of 5 by from Keap1 endogeno in cellule HEK-293THo acquistato questo anticorpo (sc-365626) per la ricerca di Keap1 endogeno in lisato totale di cellule HEK-293T. Nonostante sia presente una banda aspecifica di alto peso molecolare, l'espressione basale di Keap1 (freccia) viene efficacemente evidenziata.
Date published: 2023-02-20
Rated 5 out of 5 by from Good for WBTwo specific band (around 60 kDa) were observed in human dental pulp cells. This antibody works well for WB.
Date published: 2018-12-28
Rated 1 out of 5 by from Did not detect mouse Keap1Unfortunately, the antibody 1:500 did not detect any mouse Keap1 on Western blot (50 µg loaded) of HT22 cells.
Date published: 2018-02-23
Rated 5 out of 5 by from Strong band in WB in human breast cancer cell lineKeep1 had strong band in WB in human breast cancer cell line t47d
Date published: 2018-02-01
Rated 4 out of 5 by from Buono funzionamentoTestato su cellule HeLa per Western-Blot (1:500, 5% milk). 2 bande specifiche al altezza giusta ma segnale un po flebile.
Date published: 2017-11-07
Rated 3 out of 5 by from Good for WBTwo strong close bands for WB in human cell samples
Date published: 2017-10-12
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Keap1 抗体 (G-2) is rated 4.4 out of 5 by 25.
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