Date published: 2025-9-6
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Keap1 Antibody (F-10) - Immunohistochemistry - Image 353021
Keap1 抗体 (F-10): sc-514914. 福尔马林固定, 石蜡包埋的人甲状腺组织的免疫过氧化物酶染色显示腺细胞的胞浆和核染色.
Keap1 抗体 (F-10): sc-514914. 福尔马林固定, 石蜡包埋的人甲状腺组织的免疫过氧化物酶染色显示腺细胞的胞浆和核染色.

Keap1 抗体 (F-10): sc-514914

4.2(6)
寫評論提問

说明书
  • Keap1抗体(F-10)是小鼠单克隆IgG2b κ, 在33篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Keap1的N-terminus附近的氨基酸39-65之间
  • 抗-Keap1 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Keap1
  • 抗Keap1抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Keap1 (F-10): sc-514914的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是Keap1 抗体 (F-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Keap1 抗体 (F-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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研究信息

関連項目

Keap1抗体（F-10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的Keap1。抗Keap1抗体（F-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Keap1，即Kelch样ECH相关蛋白1，作为Cullin3依赖性E3泛素连接酶复合物的应力感应适配器发挥着至关重要的作用，该复合物对于调节Nrf2（NF-E2相关因子2）至关重要。Keap1和Nrf2之间的相互作用对于维持细胞内环境至关重要，因为Keap1在正常情况下会负向调节Nrf2，防止Nrf2在细胞核中积聚。当暴露于氧化应激或电离子时，Keap1会发生构象变化，导致Nrf2释放并随后激活，使Nrf2能够转移到细胞核并启动细胞保护基因的表达。Keap1第273和288位的半胱氨酸残基对于抑制Nrf2在细胞核中的积累至关重要，这凸显了翻译后修饰在这一通路调节中的重要性。了解Keap1-Nrf2系统维持的平衡有助于探索旨在增强细胞防御氧化损伤机制的治疗策略。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Keap1 抗体 (F-10)

sc-514914
200 µg/ml
$316.00

Keap1 (F-10): m-IgG2b BP-HRP 套装

sc-548806
200 µg Ab; 10 µg BP
$354.00

Keap1 抗体 (F-10) AC

sc-514914 AC
500 µg/ml, 25% agarose
$416.00

Keap1 抗体 (F-10) HRP

sc-514914 HRP
200 µg/ml
$316.00

Keap1 抗体 (F-10) FITC

sc-514914 FITC
200 µg/ml
$330.00

Keap1 抗体 (F-10) PE

sc-514914 PE
200 µg/ml
$343.00

Keap1 抗体 (F-10) Alexa Fluor® 488

sc-514914 AF488
200 µg/ml
$357.00

Keap1 抗体 (F-10) Alexa Fluor® 546

sc-514914 AF546
200 µg/ml
$357.00

Keap1 抗体 (F-10) Alexa Fluor® 594

sc-514914 AF594
200 µg/ml
$357.00

Keap1 抗体 (F-10) Alexa Fluor® 647

sc-514914 AF647
200 µg/ml
$357.00

Keap1 抗体 (F-10) Alexa Fluor® 680

sc-514914 AF680
200 µg/ml
$357.00

Keap1 抗体 (F-10) Alexa Fluor® 790

sc-514914 AF790
200 µg/ml
$357.00

Keap1 (F-10) 中和勝肽

sc-514914 P
100 µg/0.5 ml
$68.00

How much is the antibody of Keap1 (F-10): sc-514914. Would the free 10ug be available to help us work out some issues?

Asked by: Allan
Thank you for your question. Current pricing is available on our website. Please contact our Customer Care Department for a formal quotation. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-26

How much is shipping for https://www.scbt.com/scbt/product/keap1-antibody-f-10 ? Does this need a secondary antibody? If so can you suggest which one?

Asked by: Allan
This primary antibody ships Express Ambient via FedEx Express standard Overnight. Please refer to the link below for current shipping information. https://www.scbt.com/scbt/customer-care/shipping-information If you purchase the unconjugated mouse monoclonal antibody, sc-514914, then we recommend using our mouse IgG binding protein as a secondary antibody for detection (e.g., sc-516102). We can recommend a specific conjugated secondary antibody based on your intended research application. Please contact Technical Service for support.
Answered by: Technical Support
Date published: 2019-01-26

What application is the blocking peptide sc-514914 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from GoodGreat antibody for WB, works in mouse RAW 264.7 cells
Date published: 2025-03-18
Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:500 for overnight @ 4oC.
Date published: 2024-12-10
Rated 3 out of 5 by from Good detection for Keap1 antibodyThe antibody request 30-50 µg of cell lysates and is suitable for many cell lines. Good quality
Date published: 2018-08-28
Rated 3 out of 5 by from Weak detection via Western blotWeak detection via Western blot in human breast cancer cell lines.
Date published: 2018-04-24
Rated 4 out of 5 by from 挺好的一个月前申请的小样，western blot鉴定效果还不错，条带清晰，挺好的！！！！！！！！！！！！
Date published: 2018-03-04
Rated 5 out of 5 by from ExcellentIt is an excellent antibody, it works perfectly in WB in protein of Hela cells. Strong single band can be observed.
Date published: 2017-01-18
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Keap1 抗体 (F-10) is rated 4.2 out of 5 by 6.
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