Date published: 2025-9-7
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IP3R-I/II/III Antibody (B-2) - Immunohistochemistry - Image 286532
IP3R-I/II/III 抗体 (B-2): sc-377518. 福尔马林固定, 石蜡包埋的人小脑组织的免疫过氧化物酶染色, 显示浦肯野细胞的胞浆染色.
IP3R-I/II/III 抗体 (B-2): sc-377518. 福尔马林固定, 石蜡包埋的人小脑组织的免疫过氧化物酶染色, 显示浦肯野细胞的胞浆染色.

IP3R-I/II/III 抗体 (B-2): sc-377518

4.1(13)
寫評論提問

说明书
  • IP3R-I/II/III 抗体 B-2 是小鼠单克隆 IgG2b，IP3R-I/II/III抗体, 在27篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列2402-2701位于human物种的IP3R-II的C-terminus
  • IP3R-I/II/III 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 IP3R-I, IP3R-II and IP3R-III
  • 抗IP3R-I/II/III抗体（B-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IP3R-I/II/III (B-2): sc-377518的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是IP3R-I/II/III 抗体 (B-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与IP3R-I/II/III 抗体 (B-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 IP3R 抗体包括 IP3R-I, IP3R-II 和 IP3R-III

IP3R-I/II/III抗体（B-2）是一种IgG2b小鼠单克隆IP3R-I/II/III抗体（也称为INSP3R1、IP3R1、IP3R抗体、IP3R2抗体、INSP3R2抗体、IP3R3抗体、INSP3R3抗体、肌醇1,4,5-三磷酸细胞内受体抗体、肌醇1,4,5-三磷酸受体抗体、内质网钙通道抗体、C端钙通道/N端配体结合受体抗体，用于检测肌醇1,4,5-三磷酸或外分泌、能量代谢和生长相关抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的IP3R-I/II/III蛋白。IP3R-I/II/III抗体（B-2）既有非偶联抗IP3R-I/II/III抗体形式，也有多种偶联形式的抗IP3R-I/II/III抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌醇1,4,5-三磷酸（IP3）作为第二信使，对多种细胞外刺激物（包括激素、生长因子和神经递质）起作用。受体酪氨酸激酶通过激活磷脂酶（如磷脂酶C（PLC））间接增加细胞内IP3水平，将磷脂酰肌醇-4,5-二磷酸转化为IP3和二酰甘油（DAG）。肌醇1,4,5-三磷酸受体（IP3R）在多种细胞类型中作为肌醇三磷酸（IP3）门控钙释放通道起作用。已描述了三种IP3受体亚型，分别命名为IP3R-I、IP3R-II和IP3R-III。IP3R-I是神经元组织和中枢神经系统中表达的主要IP3R亚型，但在肝脏中也有高水平表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IP3R-I/II/III 抗体 (B-2) 参考文献:

  1. 短暂局灶性脑缺血后大鼠脑内 IP3 受体和 IP3-3-kinase mRNA 表达的原位杂交。  |  Zhang, SX., et al. 1995. Brain Res Mol Brain Res. 32: 252-60. PMID: 7500836
  2. 果蝇中 1,4,5-三磷酸肌醇受体的表达表明 IP3 信号在肌肉发育和成体化学感觉功能中发挥作用。  |  Raghu, P. and Hasan, G. 1995. Dev Biol. 171: 564-77. PMID: 7556937
  3. WB 大鼠肝上皮细胞中 I 型和 III 型三磷酸肌醇受体的异构体。  |  Joseph, SK., et al. 1995. J Biol Chem. 270: 23310-6. PMID: 7559486
  4. 在胰岛, 肾脏, 胃肠道和其他组织中表达的第三种三磷酸肌醇受体亚型 IP3R-3 的序列和功能特征。  |  Blondel, O., et al. 1993. J Biol Chem. 268: 11356-63. PMID: 8388391
  5. 与 1,4,5-三磷酸肌醇受体-FKBP12 复合物缔合作用的钙调素调节 Ca2+ 通量。  |  Cameron, AM., et al. 1995. Cell. 83: 463-72. PMID: 8521476
  6. 缺乏 1 型肌醇-1,4,5-三磷酸受体的小鼠共济失调和癫痫发作。  |  Matsumoto, M., et al. 1996. Nature. 379: 168-71. PMID: 8538767
  7. 通过酪氨酸磷酸化调节 1,4,5-三磷酸肌醇受体。  |  Jayaraman, T., et al. 1996. Science. 272: 1492-4. PMID: 8633244
  8. 淋巴细胞凋亡：3 型 1,4,5-三磷酸肌醇受体增加的调解作用。  |  Khan, AA., et al. 1996. Science. 273: 503-7. PMID: 8662540

订购信息

产品名称产品编号规格价格数量收藏夹

IP3R-I/II/III 抗体 (B-2)

sc-377518
200 µg/ml
$316.00

IP3R-I/II/III (B-2): m-IgG2b BP-HRP 套装

sc-548766
200 µg Ab; 10 µg BP
$354.00

IP3R-I/II/III 抗体 (B-2) AC

sc-377518 AC
500 µg/ml, 25% agarose
$416.00

IP3R-I/II/III 抗体 (B-2) HRP

sc-377518 HRP
200 µg/ml
$316.00

IP3R-I/II/III 抗体 (B-2) FITC

sc-377518 FITC
200 µg/ml
$330.00

IP3R-I/II/III 抗体 (B-2) PE

sc-377518 PE
200 µg/ml
$343.00

IP3R-I/II/III 抗体 (B-2) Alexa Fluor® 488

sc-377518 AF488
200 µg/ml
$357.00

IP3R-I/II/III 抗体 (B-2) Alexa Fluor® 546

sc-377518 AF546
200 µg/ml
$357.00

IP3R-I/II/III 抗体 (B-2) Alexa Fluor® 594

sc-377518 AF594
200 µg/ml
$357.00

IP3R-I/II/III 抗体 (B-2) Alexa Fluor® 647

sc-377518 AF647
200 µg/ml
$357.00

IP3R-I/II/III 抗体 (B-2) Alexa Fluor® 680

sc-377518 AF680
200 µg/ml
$357.00

IP3R-I/II/III 抗体 (B-2) Alexa Fluor® 790

sc-377518 AF790
200 µg/ml
$357.00

저희는 IP3R의 isoform type과 관계없이 C-terminal detection을 하고 싶습니다. 혹시 type 1,2,3에 따라서 band intensity가 달라질 수 있나요?

Asked by: MBIL
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2025-08-17

IP3R-I/II/III 抗体 (B-2): sc-377518此抗体是ITPR1/2/3这三型蛋白都能结合么？

Asked by: marynaxj
感谢您的提问。都能检测的。更多问题，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. Yes, recommended for detection of IP3R-I, IP3R-II and IP3R-III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-10-30

Is antigen retrieval necessary when using this antibody in IHC(P)?

Asked by: AbPolly
Thank you for your question. We do recommend antigen retrieval when working with paraffin-embedded sections, specifically sodium citrate buffer, pH 6.0, and heat. Our full protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service
Date published: 2017-02-03
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Rated 3 out of 5 by from It worksUsed in total lysates at 1:250 dilution in blocking solution overnight at 4C.
Date published: 2021-12-02
Rated 3 out of 5 by from Works, but not super strongUsed in H9 hESC and HEK293T lysates to optimise this and other antibodies. Used at 1:1000 dilution in 5% milk in TBST for 24hrs at 4C.
Date published: 2021-08-24
Rated 1 out of 5 by from No signal in WBI tested this antibody on mouse and human whole cell lysates and no signal was detected.
Date published: 2020-11-18
Rated 1 out of 5 by from This antibody does not recognize IP3R-IIIWe tried this antibody using lysates from cells expressing R1 only, R2 only, R3 only. Conclusion: this antibody recognized R2 very strongly but did not recognize R3 at all ( Human R3 or rat R3). Also, it recognized over-expressed R1 very very weakly. Therefore, it is not a pan-antibody.
Date published: 2017-09-21
Rated 5 out of 5 by from Very good IP3R antibody. I tested in WB and IF.very good IP3R antibody. I tested in WB(1:500) and IF(1:50)，it works very well!
Date published: 2017-08-26
Rated 5 out of 5 by from clean blot seenclean blot seen,you can tried 1:400 to 1:1000 dilution,this conceration is enough detect IP3R-I/II/III ,the target at above250kda, recommend use 6% SDS-PAGE.
Date published: 2017-06-25
Rated 5 out of 5 by from A good antibodyWe use this as a loading control for WB, It always very good quality
Date published: 2017-06-01
Rated 5 out of 5 by from reommend useThis antibody gave strong and clear detection of IP3R-I/II/III , allowing us to trace translocation from the cytoplasm to nucleus by IF with a standard secondary antibody.
Date published: 2017-02-05
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IP3R-I/II/III 抗体 (B-2) is rated 4.1 out of 5 by 13.
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