IP3R-I/II/III抗体 (B-2) 推荐用于 mouse, rat 和 human 来源的IP3R-I, IP3R-II and IP3R-III WB, IP, IF, IHC(P) 和 ELISA检测

IP3R-I/II/III 抗体 (B-2): sc-377518

IP3R-I/II/III抗体 (B-2) is rated 4.2 out of 5 by 12.
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说明书

    • 抗-IP3R-I/II/III 抗体 B-2 是小鼠单克隆 IgG2bIP3R-I/II/III抗体, 在12篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列2402-2701位于human物种的IP3R-II的C-terminus
    • 抗-IP3R-I/II/III 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 IP3R-I, IP3R-II and IP3R-III
    • 抗-IP3R-I/II/III 抗体 (B-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取IP3R-I/II/III (B-2): sc-377518免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG2b BP-HRP 是IP3R-I/II/III Antibody (B-2) for WB and IHC(P)首选应用二抗. 这些试剂现在以套装形式提供IP3R-I/II/III Antibody (B-2) (见下方订购信息).
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IP3R-I/II/III 抗体 (B-2): sc-377518此抗体是ITPR1/2/3这三型蛋白都能结合么?

Asked by: marynaxj
感谢您的提问。都能检测的。更多问题,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. Yes, recommended for detection of IP3R-I, IP3R-II and IP3R-III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-10-30

Is antigen retrieval necessary when using this antibody in IHC(P)?

Asked by: AbPolly
Thank you for your question. We do recommend antigen retrieval when working with paraffin-embedded sections, specifically sodium citrate buffer, pH 6.0, and heat. Our full protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service
Date published: 2017-02-03
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Rated 3 out of 5 by from Works, but not super strong Used in H9 hESC and HEK293T lysates to optimise this and other antibodies. Used at 1:1000 dilution in 5% milk in TBST for 24hrs at 4C.
Date published: 2021-08-24
Rated 1 out of 5 by from No signal in WB I tested this antibody on mouse and human whole cell lysates and no signal was detected.
Date published: 2020-11-18
Rated 1 out of 5 by from This antibody does not recognize IP3R-III We tried this antibody using lysates from cells expressing R1 only, R2 only, R3 only. Conclusion: this antibody recognized R2 very strongly but did not recognize R3 at all ( Human R3 or rat R3). Also, it recognized over-expressed R1 very very weakly. Therefore, it is not a pan-antibody.
Date published: 2017-09-21
Rated 5 out of 5 by from Very good IP3R antibody. I tested in WB and IF. very good IP3R antibody. I tested in WB(1:500) and IF(1:50),it works very well!
Date published: 2017-08-26
Rated 5 out of 5 by from clean blot seen clean blot seen,you can tried 1:400 to 1:1000 dilution,this conceration is enough detect IP3R-I/II/III ,the target at above250kda, recommend use 6% SDS-PAGE.
Date published: 2017-06-25
Rated 5 out of 5 by from A good antibody We use this as a loading control for WB, It always very good quality
Date published: 2017-06-01
Rated 5 out of 5 by from reommend use This antibody gave strong and clear detection of IP3R-I/II/III , allowing us to trace translocation from the cytoplasm to nucleus by IF with a standard secondary antibody.
Date published: 2017-02-05
Rated 5 out of 5 by from Good IP3R-I/II/III antibody Strong staining observed in IHC(P) and IF, and strong signal obtained in WB with endogenous and transfected samples. Great antibody for many applications.
Date published: 2017-01-30
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