IKK alpha Antibody (D-5): sc-136978

IKKα Antibody (D-5) is a mouse monoclonal IgG2a antibody that detects IKKα in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). IKKα, also known as CHUK, plays a pivotal role in activating the transcription factor NFκB, which regulates immune responses and inflammation. Located primarily in the cytoplasm, IKKα interacts with the inhibitory protein IκB, facilitating phosphorylation on specific serine residues. This post-translational modification leads to IκB degradation, allowing NFκB to translocate to the nucleus and initiate transcription of target genes involved in inflammatory responses. IKKα′s importance in this signaling pathway is underscored by interaction with NFκB inducing kinase (NIK), which acts as a central regulator for NFκB activation in response to proinflammatory cytokines such as tumor necrosis factor (TNF) and interleukin-1 (IL-1). The functional IKK complex, comprising IKKα, IKKβ, and IKKγ (NEMO), is critical for IκB phosphorylation, highlighting essential contributions of each subunit in NFκB signaling regulation. Anti-IKKα antibody (D-5) serves as an invaluable tool for researchers studying inflammation and immune response mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IKK alpha Antibody (D-5) References:

1. IKKα restoration via EZH2 suppression induces nasopharyngeal carcinoma differentiation.  |  Yan, M., et al. 2014. Nat Commun. 5: 3661. PMID: 24739462
2. TRIM22 can activate the noncanonical NF-κB pathway by affecting IKKα.  |  Qiu, H., et al. 2015. J Recept Signal Transduct Res. 35: 289-94. PMID: 25510414
3. Epithelial IKKα licenses ILC3s to defend the intestinal barrier.  |  Eftychi, C. and Pasparakis, M. 2015. J Exp Med. 212: 1483. PMID: 26392427
4. IKKα is required in the intestinal epithelial cells for tumour stemness.  |  Colomer, C., et al. 2018. Br J Cancer. 118: 839-846. PMID: 29438366
5. INH14, a Small-Molecule Urea Derivative, Inhibits the IKKα/β-Dependent TLR Inflammatory Response.  |  Drexel, M., et al. 2019. Chembiochem. 20: 710-717. PMID: 30447158
6. Selective degradation of IKKα by autophagy is essential for arsenite-induced cancer cell apoptosis.  |  Tan, Q., et al. 2020. Cell Death Dis. 11: 222. PMID: 32265434
7. MxA suppresses TAK1-IKKα/β-NF-κB mediated inflammatory cytokine production to facilitate Mycobacterium tuberculosis infection.  |  Zhou, X., et al. 2020. J Infect. 81: 231-241. PMID: 32445727
8. Autophagic feedback-mediated degradation of IKKα requires CHK1- and p300/CBP-dependent acetylation of p53.  |  Xu, X., et al. 2020. J Cell Sci. 133: PMID: 33097607
9. Dehydrocostus Lactone Suppresses Dextran Sulfate Sodium-Induced Colitis by Targeting the IKKα/β-NF-κB and Keap1-Nrf2 Signalling Pathways.  |  Yuan, Y., et al. 2022. Front Pharmacol. 13: 817596. PMID: 35321327
10. IKKα-Mediated Noncanonical NF-κB Signaling Is Required To Support Murine Gammaherpesvirus 68 Latency In Vivo.  |  Cieniewicz, B., et al. 2022. J Virol. 96: e0002722. PMID: 35481781

The preferred IKK alpha antibody is IKK alpha Antibody (B-8): sc-7606.