HSP 27抗体 (F-4) 推荐用于 mouse, rat 和 human 来源的HSP 27 WB, IP, IF, IHC(P) 和 ELISA检测

HSP 27 抗体 (F-4): sc-13132

HSP 27抗体 (F-4) is rated 4.7 out of 5 by 35.
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说明书

    • 抗-HSP 27 抗体 F-4 是小鼠单克隆 IgG2a κ,HSP 27抗体, 在96篇文献中引用,规格为200 µg/ml
    • 免疫human物种的HSP 27的氨基酸32-108
    • 抗-HSP 27 抗体 (F-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 HSP 27
    • 抗-HSP 27 抗体 (F-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取HSP 27 (F-4): sc-13132免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HSP 27 Antibody (F-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with HSP 27 Antibody (F-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the best secondary to use with this antibody in Western Blot?

Asked by: TinTin
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2018-03-15
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Rated 2 out of 5 by from Funzionamento discreto Anticorpo testato. Riconosce la proteina over-espressa, meno bene quella endogena
Date published: 2020-07-09
Rated 4 out of 5 by from recommended for WB Works very well for whole cell lysates of HEp-2 and RKO cells when the antibody is diluted 1:500 in BSA/PBS-T. For IF (cells were fixed in 2% formaldehyde) we had to dilute the antibody 1:50 to achieve a good cytoplasmic staining.
Date published: 2020-01-09
Rated 5 out of 5 by from SC-13132 The antibody shows sensitive and specific in IHC.
Date published: 2019-11-21
Rated 5 out of 5 by from perfetto ho provato l'anticorpo come campione gratuito. L'ho usato in western blot su campioni di cellule di ratto, diluito 1:1000 in BSA 5%.
Date published: 2019-10-31
Rated 5 out of 5 by from western blotting Worked well with western blotting at 1:600 dilution in HepG2 Cells.
Date published: 2018-12-24
Rated 3 out of 5 by from WB in HeLa, KM-12 and LIM1215 Works fine with HeLa (50 µg) but no signal in other tested cell lines, Tested both 1:1000 and 1:500 dilutions in 5% Milk/TBST giving a little bit of background.
Date published: 2018-11-26
Rated 5 out of 5 by from Very specific and clear band Very specific and clear signal in western blotting (1:1000) of different type of cells: HeLa, C2C12, 18-HBE, HEPG-2
Date published: 2018-07-19
Rated 5 out of 5 by from Detecting mouse HSP27 protein by WB using sc-13132 Western blotting conditions: Species: Mouse (C57BL6) Tissue: Skeletal muscles Protein amount: 10ug SDS-PAGE gel%: 4-20% gradient gel or 16% gel Blocking buffer: 5% skim milk in PBS Primary antibody dilution: 1:1000 (1:500 - 1:2000 works well) in 5% milk-PBS Washing buffer: TBS - TBST - TBS Secondary antibody detection methods: HRP-conjugated in 5% milk-PBS Band detected: below the 30 kDa protein marker, corresponding to the 27 kDa size of this protein Film and Li-cor exposure: 5s and 15s, respectively Precautions: HSP27 is quite abundantly expressed in murine skeletal muscles and sc-13132 detects this protein very well. If you load a very high amount of protein and use a high primary antibody amount, upon using the HRP-substrate you might get a 'burn-through effect' due to the signal generated and bands might begin to appear hollow. Overall, the blot quality generated using this antibody and the above mentioned conditions was excellent!
Date published: 2018-06-08
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