Date published: 2025-9-6
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ERK 5 Antibody (C-7) - Western Blotting - Image 354697
ERK 5 抗体 (C-7): sc-398015. Caki-1 (A), Hep G2 (B), RAW 264. 7 (C), A-10 (D) 和 PC-12 (E) 全细胞裂解液中 ERK 5 表达的 Western 印迹分析.
ERK 5 抗体 (C-7): sc-398015. Caki-1 (A), Hep G2 (B), RAW 264. 7 (C), A-10 (D) 和 PC-12 (E) 全细胞裂解液中 ERK 5 表达的 Western 印迹分析.

ERK 5 抗体 (C-7): sc-398015

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说明书
  • ERK 5 抗体 C-7 是小鼠单克隆 IgG1 κ，ERK 5抗体, 在16篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的ERK 5的C-terminus附近的氨基酸671-698之间
  • ERK 5 抗体 (C-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ERK 5; 也和以下物种反应，包括： 和 canine and bovine
  • 抗ERK 5抗体（C-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ERK 5 (C-7): sc-398015的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 ERK 5 抗体 (C-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ERK 5 抗体 (C-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ERK 抗体 包括 ERK 1, ERK 2, ERK 3和ERK 5

ERK 5 抗体（C-7）是一种 IgG1 κ 小鼠单克隆 ERK 5 抗体（也称为 ERK 5 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人的 ERK 5 蛋白。ERK 5 抗体（C-7）既可以是非偶联的抗 ERK 5 抗体形式，也可以是多种偶联形式的抗 ERK 5 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。生长因子、激素和神经递质对信号转导途径的激活是通过两种密切相关的MAP激酶p44和p42介导的，这两种激酶分别被称为细胞外信号相关激酶1（ERK1）和ERK2。ERK蛋白通过在一个特征性的Thr-Glu-Tyr基序内特定的酪氨酸和苏氨酸位点上的双重磷酸化来调节。苏氨酸（Thr）和酪氨酸（Tyr）残基上的磷酸化对于酶的完全激活是必需的。作为对激活的反应，MAP激酶磷酸化丝氨酸和苏氨酸的下游成分。MAP激酶的上游调节因子包括MAP激酶激酶（MEK）、MEK激酶和Raf-1。ERK家族还有三个额外的成员：ERK3、ERK5和ERK6。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ERK 5 抗体 (C-7) 参考文献:

  1. ERK1和ERK2是两种微管相关蛋白2激酶，它们在原位介导酪氨酸羟化酶丝氨酸-31的磷酸化。  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
  2. 纯化磷酸化并激活 Erk-1 基因产物的鼠蛋白酪氨酸/苏氨酸激酶：与裂殖酵母 byr1 基因产物的关系。  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
  3. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  4. 细胞外信号调节激酶 1（一种胰岛素刺激的微管相关蛋白 2 激酶）的纯化和特性。  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
  5. 确定 pp42/丝裂原活化蛋白激酶（MAP 激酶）的调节磷酸化位点。  |  Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
  6. ERK：丝氨酸/苏氨酸蛋白激酶家族，在胰岛素和 NGF 的作用下被激活并酪氨酸磷酸化。  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
  7. ERK5 及其在肿瘤发生中的作用。  |  Lochhead, PA., et al. 2012. Biochem Soc Trans. 40: 251-6. PMID: 22260700
  8. MEKK3-MEK5-ERK5 信号传导促进线粒体降解。  |  Craig, JE., et al. 2020. Cell Death Discov. 6: 107. PMID: 33101709
  9. 有氧运动改变黑色素瘤微环境并调节 ERK5 S496 磷酸化  |  Savage, H., et al. 2023. Cancer Immunol Res. 11: 1168-1183. PMID: 37307577
  10. 作为 ERK5 抑制剂治疗癌症的咪唑吡啶化合物  |  Sabnis, RW. 2024. ACS Med Chem Lett. 15: 777-778. PMID: 38894904
  11. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  12. 参与 C2C12 肌母细胞分化的有丝分裂原激活蛋白激酶 ERK6  |  Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070

订购信息

产品名称产品编号规格价格数量收藏夹

ERK 5 抗体 (C-7)

sc-398015
200 µg/ml
$316.00

ERK 5 (C-7): m-IgG Fc BP-HRP 套装

sc-527730
200 µg Ab; 10 µg BP
$354.00

ERK 5 (C-7): m-IgG1 BP-HRP 套装

sc-533103
200 µg Ab; 20 µg BP
$354.00

ERK 5 抗体 (C-7) AC

sc-398015 AC
500 µg/ml, 25% agarose
$416.00

ERK 5 抗体 (C-7) HRP

sc-398015 HRP
200 µg/ml
$316.00

ERK 5 抗体 (C-7) FITC

sc-398015 FITC
200 µg/ml
$330.00

ERK 5 抗体 (C-7) PE

sc-398015 PE
200 µg/ml
$343.00

ERK 5 抗体 (C-7) Alexa Fluor® 488

sc-398015 AF488
200 µg/ml
$357.00

ERK 5 抗体 (C-7) Alexa Fluor® 546

sc-398015 AF546
200 µg/ml
$357.00

ERK 5 抗体 (C-7) Alexa Fluor® 594

sc-398015 AF594
200 µg/ml
$357.00

ERK 5 抗体 (C-7) Alexa Fluor® 647

sc-398015 AF647
200 µg/ml
$357.00

ERK 5 抗体 (C-7) Alexa Fluor® 680

sc-398015 AF680
200 µg/ml
$357.00

ERK 5 抗体 (C-7) Alexa Fluor® 790

sc-398015 AF790
200 µg/ml
$357.00

ERK 5 (C-7) 中和勝肽

sc-398015 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-398015 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 10T1/2 cell lysatesIt works well in 1:500. I get a clear results for paper.
Date published: 2017-10-05
Rated 5 out of 5 by from ERK5 antibodyi used the antibody for western blot and it worked fine
Date published: 2017-10-02
Rated 5 out of 5 by from Detected ERK5 band in T98G cell lysatesTested this antibody in T98G cell lysates and was able to detect (via western blot) ERK5 in untreated cells as well as a shift when cells were treated with EGF prior to lysing.
Date published: 2017-08-30
Rated 3 out of 5 by from Good for IPWe were unable to detect the signal in HUVEC extract or Mesothelioma cells with out IP
Date published: 2017-07-03
Rated 1 out of 5 by from Didnot work for WBDid not work for WB. Used the antibody at 1:1000 dilution for MDA-MB-231 cell lysate.
Date published: 2017-06-07
Rated 4 out of 5 by from Nice productIt has a few non-specific bands but didn't affect the result.
Date published: 2017-04-18
Rated 4 out of 5 by from ottimo anticorpoHo acquistato questo articolo per immunoistochimica e devo dire che è molto buono, da un segnale pulito
Date published: 2017-04-11
Rated 5 out of 5 by from Great Antibody for Tissue StainingThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-27
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ERK 5 抗体 (C-7) is rated 4.0 out of 5 by 9.
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