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CYP1A1 抗体 (A-9): sc-393979

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说明书
  • CYP1A1 抗体 A-9 是小鼠单克隆 IgG1 κ,CYP1A1抗体, 在27篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的CYP1A1的C-terminus附近的氨基酸479-506之间
  • CYP1A1 抗体 (A-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CYP1A1
  • 抗CYP1A1抗体(A-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CYP1A1 Antibody (A-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CYP1A1 Antibody (A-9) 打包提供(请参阅下面的订购信息)。
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関連項目

CYP1A1抗体(A-9)是一种IgG1 κ小鼠单克隆CYP1A1抗体(也称为细胞色素P450 1A1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CYP1A1蛋白。CYP1A1抗体(A-9)既可以是非偶联抗CYP1A1抗体形式,也可以是多种偶联形式的抗CYP1A1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。P450酶构成单加氧酶酶家族,参与多种内源性和外源性化合物的代谢。几种P450酶已被分类为序列相似的CYP1A和CYP2A亚家族成员。NADPH细胞色素P450还原酶是一种微粒体酶,负责在P450催化循环中将电子从NADPH转移到细胞色素P450酶。NADPH细胞色素P450还原酶定位于内质网,在那里它还能够将电子转移到血红素加氧酶和细胞色素b5。NADPH细胞色素P450还原酶在结构上与两个不同的黄素蛋白家族、铁氧还蛋白核苷酸还原酶(FNR)和黄素蛋白有关。NADPH细胞色素P450还原酶的电子转移需要两个黄素辅因子FAD和FMN分别与FNR和黄素蛋白结构域结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CYP1A1 抗体 (A-9) 参考文献:

  1. 血清可增加3-甲基胆蒽对CYP1A1的诱导作用。  |  N'Guyen, QB., et al. 2002. Biochem Biophys Res Commun. 297: 249-54. PMID: 12237110
  2. CYP1A1、CYP3A5和CYP3A7多态性与睾丸癌易感性。  |  Kristiansen, W., et al. 2011. Int J Androl. 34: 77-83. PMID: 20345875
  3. CYP1A1多态性及其在卵巢癌和子宫内膜癌中的转录调控的重要性。  |  Mrozikiewicz, PM., et al. 2011. Ginekol Pol. 82: 925-32. PMID: 22384629
  4. CYP1A1蛋白活性与翼状胬肉组织和细胞中的等位基因变异有关。  |  Peng, ML., et al. 2012. Mol Vis. 18: 1937-43. PMID: 22876118
  5. CYP1A1和Cnr硝基还原酶在体外生物活化氯硝柳胺。  |  Beristain-Castillo, E., et al. 2013. Mutagenesis. 28: 645-51. PMID: 23956140
  6. 子宫内膜肿瘤中 CYP1A1 和 CYP1B1 酶的表达谱。  |  Spyrou, I., et al. 2014. Tumour Biol. 35: 9549-56. PMID: 24957043
  7. CYP1A1多态性与肝细胞癌之间的关联:荟萃分析。  |  Yu, BW., et al. 2015. Genet Mol Res. 14: 1076-84. PMID: 25730047
  8. 宫颈癌中CYP1A1和COMT多态性的表观相互作用。  |  Matos, A., et al. 2016. Oxid Med Cell Longev. 2016: 2769804. PMID: 26798414
  9. GDC-0339中CYP1A1介导的氨基氮杂环庚烷分子内重排。  |  Takahashi, RH., et al. 2017. Drug Metab Dispos. 45: 1084-1092. PMID: 28790146
  10. 羟色胺是一种通过 AhR 调节肠道 CYP1A1 的内源性调节剂。  |  Manzella, C., et al. 2018. Sci Rep. 8: 6103. PMID: 29666456

订购信息

产品名称产品编号规格价格数量收藏夹

CYP1A1 抗体 (A-9)

sc-393979
200 µg/ml
$322.00

CYP1A1 (A-9): m-IgG Fc BP-HRP 套装

sc-530649
200 µg Ab; 10 µg BP
$361.00

CYP1A1 (A-9): m-IgGκ BP-HRP 套装

sc-524165
200 µg Ab, 40 µg BP
$361.00

CYP1A1 (A-9): m-IgG1 BP-HRP 套装

sc-544178
200 µg Ab; 20 µg BP
$361.00

CYP1A1 抗体 (A-9) AC

sc-393979 AC
500 µg/ml, 25% agarose
$424.00

CYP1A1 抗体 (A-9) HRP

sc-393979 HRP
200 µg/ml
$322.00

CYP1A1 抗体 (A-9) FITC

sc-393979 FITC
200 µg/ml
$336.00

CYP1A1 抗体 (A-9) PE

sc-393979 PE
200 µg/ml
$349.00

CYP1A1 抗体 (A-9) Alexa Fluor® 488

sc-393979 AF488
200 µg/ml
$364.00

CYP1A1 抗体 (A-9) Alexa Fluor® 546

sc-393979 AF546
200 µg/ml
$364.00

CYP1A1 抗体 (A-9) Alexa Fluor® 594

sc-393979 AF594
200 µg/ml
$364.00

CYP1A1 抗体 (A-9) Alexa Fluor® 647

sc-393979 AF647
200 µg/ml
$364.00

CYP1A1 抗体 (A-9) Alexa Fluor® 680

sc-393979 AF680
200 µg/ml
$364.00

CYP1A1 抗体 (A-9) Alexa Fluor® 790

sc-393979 AF790
200 µg/ml
$364.00

CYP1A1 (A-9) 中和勝肽

sc-393979 P
100 µg/0.5 ml
$69.00

HI TECHNICAL SUPPORT, CYP1A1 (A-9) in my phd progect. i found clear band for mda-mb468 on nitrocelluse membrane at dilution 1:100 incubation over night - i could not detect band in MCF-7 and mda-231 could you help me please as i want to purchas

Asked by: deem
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-05-20

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-13

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 12
Date published: 2017-01-13
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Rated 4 out of 5 by JuanSH from Great for WBWorks well for western blot in hRECs and human RCC cells.
Date published: 2022-06-08
Rated 4 out of 5 by MuGu from Worked well for human and mouse samplesWorked well when using for WB at 1:200 dilution. Provided nice clear bands in both human and mouse tissue lysates after longer exposure times.
Date published: 2018-03-22
Rated 1 out of 5 by Mirka from Not really working.. :-(Tried in WB of human hepatocytes Huh7 and Huh7.5. No bands at the expected size, only some faint bands at the bottom of the blot (maybe 25-30kDa).
Date published: 2018-01-22
Rated 5 out of 5 by 小新贝贝 from Good for WB1:200 diluted for wb of rat heart protein,there is a clear band at the position above 70KD.
Date published: 2017-10-20
Rated 5 out of 5 by winnie_wang from Nice Western BlotNice Western Blot data of CYP1A1 expression in HCC whole cell lysates.
Date published: 2017-05-03
Rated 5 out of 5 by cjMara from Excellent new antibody!There are several good CYP1A1 clones to choose from, but this new CYP1A1 (A-9) clone gives excellent results in our in-house testing by Western blotting in mouse, rat and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by Osamu from GreatGreat. It appeared as a single band at the size expected. No background bands found. I used HCC827 Lung Cancer Cells for western blot.
Date published: 2015-03-26
Rated 5 out of 5 by TO from Great single bands in WB with HeLa and NeuroGreat single bands in WB with HeLa and Neuro-2A whole cell lysates -SCBT QC
Date published: 2014-07-13
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CYP1A1 抗体 (A-9) is rated 4.3 out of 5 by 8.
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