Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
CYP1A1 Antibody (B-4) - Western Blotting - Image 3997
CYP1A1 抗体 (B-4): sc-25304. 人重组 CYP1A1 融合蛋白 (A) 和 HeLa 全细胞裂解液中 CYP1A1 表达的 Western 印迹分析 (B).
CYP1A1 Antibody (B-4) - Western Blotting - Image 3997
CYP1A1 抗体 (B-4): sc-25304. 人重组 CYP1A1 融合蛋白 (A) 和 HeLa 全细胞裂解液中 CYP1A1 表达的 Western 印迹分析 (B).
CYP1A1 Antibody (B-4) - Immunohistochemistry - Image 3995
CYP1A1 抗体 (B-4): sc-25304. 对福尔马林固定, 石蜡包埋的小鼠肝组织进行免疫过氧化物酶染色, 显示细胞质定位.
CYP1A1 Antibody (B-4) - Western Blotting - Image 21844
CYP1A1 抗体 (B-4): sc-25304. 未转染: sc-117752 (A) 和转染: sc-114027 (B) 293T 全细胞裂解液中 CYP1A1 表达的 Western 印迹分析.
CYP1A1 抗体 (B-4): sc-25304. 人重组 CYP1A1 融合蛋白 (A) 和 HeLa 全细胞裂解液中 CYP1A1 表达的 Western 印迹分析 (B).

CYP1A1 抗体 (B-4): sc-25304

4.8(10)
寫評論提問

说明书
  • CYP1A1抗体(B-4)是小鼠单克隆IgG2a κ, 在46篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CYP1A1的氨基酸246-315
  • 抗-CYP1A1 抗体 (B-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CYP1A1
  • 抗CYP1A1抗体（B-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CYP1A1 (B-4): sc-25304的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CYP1A1 抗体 (B-4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 CYP 抗体 包括 CYP11B, CYP17A1, CYP19, CYP1A1, CYP1A2, CYP1B1, CYP24, CYP26A1, CYP27A1和CYP27B1

CYP1A1抗体（B-4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的CYP1A1。抗CYP1A1抗体（B-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CYP1A1是细胞色素P450酶家族的一员，在代谢多种内源性化合物（如激素和脂肪酸）以及外源性物质（包括药物和环境毒素）方面发挥着关键作用。CYP1A1的正常运作对于有害物质解毒和维持代谢平衡至关重要，因此CYP1A1成为研究药物代谢和毒理学的重要靶标。CYP1A1主要位于肝细胞的内质网中，在那里催化底物氧化，这凸显了CYP1A1在肝脏代谢中的重要性。了解CYP1A1的表达和调节可以深入了解药物反应和毒性化合物敏感性方面的个体差异，这强调了CYP1A1在药物基因组学和个性化医学中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CYP1A1 抗体 (B-4) 参考文献:

  1. 人类肝脏 NADPH 细胞色素 P-450 还原酶的结构和功能分析：人类酶和 NADPH 结合位点的完整序列。  |  Haniu, M., et al. 1989. Biochemistry. 28: 8639-45. PMID: 2513880
  2. 通过定点突变分析 NADPH 细胞色素 P-450 氧化还原酶 FMN 结合域的结构。  |  Shen, AL., et al. 1989. J Biol Chem. 264: 7584-9. PMID: 2708380
  3. 黄酮类化合物对人类CYP1A1的抑制作用。  |  Santes-Palacios, R., et al. 2020. Toxicol In Vitro. 62: 104681. PMID: 31655123
  4. CYP1A1/1B1及相关化学预防剂对致癌物质的激活： 综述。  |  Li, Y., et al. 2021. Curr Cancer Drug Targets. 21: 21-54. PMID: 33023449
  5. CYP1A1酶活性通过调节AHR途径影响皮肤炎症。  |  Kyoreva, M., et al. 2021. J Invest Dermatol. 141: 1553-1563.e3. PMID: 33385398
  6. 抑制CYP1A1减轻秋水仙碱诱导的肝毒性。  |  Huang, R., et al. 2024. Toxins (Basel). 16: PMID: 38251251
  7. 纯化的肝脏微粒体 NADPH-细胞色素 P-450 还原酶。氧化还原状态的光谱特征。  |  Vermilion, JL. and Coon, MJ. 1978. J Biol Chem. 253: 2694-704. PMID: 632295
  8. 小鼠 NADPH-细胞色素 P-450 氧化还原酶：分子克隆和在酵母中的功能表达。  |  Ohgiya, S., et al. 1994. Biochim Biophys Acta. 1186: 137-41. PMID: 8011664
  9. NADPH-P-450 还原酶：真核生物和原核生物异构体的结构和功能比较。  |  Sevrioukova, IF. and Peterson, JA. 1995. Biochimie. 77: 562-72. PMID: 8589067
  10. P450 超家族：新序列, 基因图谱, 登录号和命名法的更新。  |  Nelson, DR., et al. 1996. Pharmacogenetics. 6: 1-42. PMID: 8845856
  11. 通过光还原法对依赖 FAD 的细胞色素 P450 还原酶活性进行定量。  |  Hodgson, AV. and Strobel, HW. 1996. Anal Biochem. 243: 154-7. PMID: 8954538
  12. P450BM-3；两个领域的故事--还是三个领域的故事？  |  Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725

订购信息

产品名称产品编号规格价格数量收藏夹

CYP1A1 抗体 (B-4)

sc-25304
200 µg/ml
$316.00

CYP1A1 抗体 (B-4) AC

sc-25304 AC
500 µg/ml, 25% agarose
$416.00

CYP1A1 抗体 (B-4) HRP

sc-25304 HRP
200 µg/ml
$316.00

CYP1A1 抗体 (B-4) FITC

sc-25304 FITC
200 µg/ml
$330.00

CYP1A1 抗体 (B-4) PE

sc-25304 PE
200 µg/ml
$343.00

CYP1A1 抗体 (B-4) Alexa Fluor® 488

sc-25304 AF488
200 µg/ml
$357.00

CYP1A1 抗体 (B-4) Alexa Fluor® 546

sc-25304 AF546
200 µg/ml
$357.00

CYP1A1 抗体 (B-4) Alexa Fluor® 594

sc-25304 AF594
200 µg/ml
$357.00

CYP1A1 抗体 (B-4) Alexa Fluor® 647

sc-25304 AF647
200 µg/ml
$357.00

CYP1A1 抗体 (B-4) Alexa Fluor® 680

sc-25304 AF680
200 µg/ml
$357.00

CYP1A1 抗体 (B-4) Alexa Fluor® 790

sc-25304 AF790
200 µg/ml
$357.00

what is difference between CYP1A1 and CYP1A1fusion, I detect fusion band very strong, but CYP1A1 very weak. In most of case I can not see band

Asked by: karick
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

I WOULD LIKE TO DO IF to detect cyp1a1 which one do you advise me cyp1A1 (B-4) OR CYP1A1 (A-9) .THANK YOU HEN

Asked by: HEN D
Thank you for your question. Both of these primary antibodies are great for IF/IHC, so you can use either one. If you want to discuss each product in detail, please reach out to Technical Service, we're happy to discuss which product would be best for your specific use.
Answered by: Tech Service
Date published: 2019-01-21

Can we use this product in chickens as immunohistochemical paraffine embedded tissue?

Asked by: burak
Thank you for your question. We do not recommend this mouse monoclonal, CYP1A1 Antibody (B-4): sc-25304, for detection of CYP1A1 in samples of chicken origin. The antibody is recommended for IHC(P), or immunohistochemistry on formalin-fixed paraffin-embedded tissue sections of mouse, rat and human origin.
Answered by: Technical Support
Date published: 2017-04-24

Is this antibody available directly conjugated to HRP?

Asked by: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using CYP1A1 (A-9): sc-393979 for CYP1A1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Technical Support 15
Date published: 2017-01-22
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_25304, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great antibody!Worked great for human and mouse tissue lysates when used at 1:200.
Date published: 2018-03-23
Rated 5 out of 5 by from this product is perfect.its very helpful with my experiments ,and I will purchase antibodies of this brand again
Date published: 2018-03-18
Rated 5 out of 5 by from Works great!We used this antibody in a western blot on human hepatoma (Huh 7) cell lysate, and we obtained a single band at the expected molecular weight. Works well!
Date published: 2018-03-01
Rated 5 out of 5 by from good resultThis antibody is supposed to detect CYP1A1 in mouse liver tissue ,the antibody dilution from 1:500 to 1:1000, the final result is expected signal.
Date published: 2017-08-19
Rated 3 out of 5 by from GoodWe bought this three month ago. Good band in western blot analysis.
Date published: 2017-06-30
Rated 5 out of 5 by from Strong IHCVery nice IHC staining data; also positive western results
Date published: 2017-01-28
Rated 5 out of 5 by from Great for IHC(P)The CYP1A1 Antibody (B-4): sc-25304 works very well for IHC with formalin fixed, paraffin-embedded tissue sections showing localized staining to the cytoplasm.
Date published: 2017-01-11
Rated 5 out of 5 by from WB: Antibody detects endogenous CYP1A1 inWB: Antibody detects endogenous CYP1A1 in HeLa samples. -SCBT QC
Date published: 2015-03-07
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_25304, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
CYP1A1 抗体 (B-4) is rated 4.8 out of 5 by 10.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_25304, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 99ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。